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Literature summary for 1.14.14.154 extracted from

  • Chen, S.H.; Sheng, C.Q.; Xu, X.H.; Jiang, Y.Y.; Zhang, W.N.; He, C.
    Identification of Y118 amino acid residue in Candida albicans sterol 14alpha-demethylase associated with the enzyme activity and selective antifungal activity of azole analogues (2007), Biol. Pharm. Bull., 30, 1246-1253.
    View publication on PubMed
Search for more literature for 1.14.14.154

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant enzymes in Saccharomyces cerevisiae Candida albicans

Protein Variants

Protein Variants Comment Organism
Y118A site-directed mutagenesis, the mutant shows reduced actalytic activity an reduced azole susceptibility compared to the wild-type enzyme Candida albicans
Y118F site-directed mutagenesis, the mutant shows reduced actalytic activity an reduced azole susceptibility compared to the wild-type enzyme Candida albicans
Y118T site-directed mutagenesis, the mutant shows reduced actalytic activity an reduced azole susceptibility compared to the wild-type enzyme Candida albicans

Inhibitors

Inhibitors Comment Organism Structure
fluconazole
-
 Candida albicans

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Candida albicans
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ a cytochrome P450 enzyme Candida albicans

Organism

Organism UniProt Comment Textmining
Candida albicans
-
-
-

Synonyms

Synonyms Comment Organism
CACYP51
-
 Candida albicans
sterol 14alpha-demethylase
-
 Candida albicans
sterol 14alpha-demethylase P450
-
 Candida albicans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Candida albicans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Candida albicans

Cofactor

Cofactor Comment Organism Structure
cytochrome a cytochrome P450 enzyme Candida albicans