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Literature summary for 1.14.14.154 extracted from

  • Cools, H.J.; Parker, J.E.; Kelly, D.E.; Lucas, J.A.; Fraaije, B.A.; Kelly, S.L.
    Heterologous expression of mutated eburicol 14alpha-demethylase (CYP51) proteins of Mycosphaerella graminicola to assess effects on azole fungicide sensitivity and intrinsic protein function (2010), Appl. Environ. Microbiol., 76, 2866-2872.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant MgCYP51 variants in a Saccharomyces cerevisiae mutant strain YUG37:erg11 carrying a doxycycline-regulatable tetO7-CYC promoter controlling native CYP51 expression. The wild-type MgCYP51 protein complements the function of the orthologous protein in the yeast, while mutants L50S, Y459D, and Y461H do not, only mutant I381V/Y459D/Y461H partially restores the activity of the yeast mutant Zymoseptoria tritici

Protein Variants

Protein Variants Comment Organism
I381V site-directed mutagenesis, the mutant enzyme does not complement the CYP-deficient Saccharomyces cerevisiae mutant strain YUG37:erg11 Zymoseptoria tritici
I381V/Y459D/Y461H site-directed mutagenesis, the mutant enzyme does partially complement the CYP-deficient Saccharomyces cerevisiae mutant strain YUG37:erg11 Zymoseptoria tritici
L50S site-directed mutagenesis, the mutant enzyme does not complement the CYP-deficient Saccharomyces cerevisiae mutant strain YUG37:erg11 Zymoseptoria tritici
additional information construction of the deletion mutant DELTAY459/G460 Zymoseptoria tritici
Y459D site-directed mutagenesis, the mutant enzyme does not complement the CYP-deficient Saccharomyces cerevisiae mutant strain YUG37:erg11 Zymoseptoria tritici
Y461H site-directed mutagenesis, the mutant enzyme does not complement the CYP-deficient Saccharomyces cerevisiae mutant strain YUG37:erg11 Zymoseptoria tritici

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
Zymoseptoria tritici
-
-

Organism

Organism UniProt Comment Textmining
Zymoseptoria tritici
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gene CYP51
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Zymoseptoria tritici IPO323
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gene CYP51
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Synonyms

Synonyms Comment Organism
CYP51
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Zymoseptoria tritici
eburicol 14alpha-demethylase
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Zymoseptoria tritici