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Literature summary for 1.14.14.154 extracted from

  • Hargrove, T.Y.; Wawrzak, Z.; Liu, J.; Nes, W.D.; Waterman, M.R.; Lepesheva, G.I.
    Substrate preferences and catalytic parameters determined by structural characteristics of sterol 14alpha-demethylase (CYP51) from Leishmania infantum (2011), J. Biol. Chem., 286, 26838-26848.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.14.154

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Leishmania infantum

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 0.1 M Tris-HCl, pH 8.0, and 9% (w/v) PEG 6000 Leishmania infantum

Inhibitors

Inhibitors Comment Organism Structure
fluconazole the inhibitor not only replaces the substrate in the enzyme active site but most likely displaces the CO from the iron coordination sphere Leishmania infantum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00911
-
 14alpha-methylzymosterol at pH 7.4 and 37°C Leishmania infantum
0.01131
-
 obtusifoliol at pH 7.4 and 37°C Leishmania infantum
0.01204
-
 lanosterol at pH 7.4 and 37°C Leishmania infantum
0.01852
-
 norlanosterol at pH 7.4 and 37°C Leishmania infantum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
 x * 54000, calculated from amino acid sequence Leishmania infantum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
14alpha-methylzymosterol + [reduced NADPH-hemoprotein reductase] + O2 Leishmania infantum
-
 ?
-
 ?
lanosterol + [reduced NADPH-hemoprotein reductase] + O2 Leishmania infantum i.e. lanosta-8,24-dien-3beta-ol 4,4-dimethylcholesta-8,14,24-trien-3-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
norlanosterol + [reduced NADPH-hemoprotein reductase] + O2 Leishmania infantum i.e. 4alpha,14alpha-dimethylcholesta-8,24-dien-3beta-ol ?
-
 ?
obtusifoliol + [reduced NADPH-hemoprotein reductase] + O2 Leishmania infantum i.e. 4alpha,14alpha-dimethyl-5'-ergosta-8,24(24')-dien-3beta-ol 4alpha-methyl-5alpha-ergosta-8,14,24(28)-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Leishmania infantum A2TEF2
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
14alpha-methylzymosterol + [reduced NADPH-hemoprotein reductase] + O2
-
 Leishmania infantum ?
-
 ?
24,25-dihydrolanosterol + [reduced NADPH-hemoprotein reductase] + O2 i.e. anosta-8-en-3ebta-ol Leishmania infantum ?
-
 ?
eburicol + [reduced NADPH-hemoprotein reductase] + O2 i.e. 24-methylenelanosta-8-en-3beta-ol Leishmania infantum ?
-
 ?
lanosterol + [reduced NADPH-hemoprotein reductase] + O2 i.e. lanosta-8,24-dien-3beta-ol Leishmania infantum 4,4-dimethylcholesta-8,14,24-trien-3-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
norlanosterol + [reduced NADPH-hemoprotein reductase] + O2 i.e. 4alpha,14alpha-dimethylcholesta-8,24-dien-3beta-ol Leishmania infantum ?
-
 ?
obtusifoliol + [reduced NADPH-hemoprotein reductase] + O2 i.e. 4alpha,14alpha-dimethyl-5'-ergosta-8,24(24')-dien-3beta-ol Leishmania infantum 4alpha-methyl-5alpha-ergosta-8,14,24(28)-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Subunits

Subunits Comment Organism
? x * 54000, calculated from amino acid sequence Leishmania infantum

Synonyms

Synonyms Comment Organism
CYP51
-
 Leishmania infantum

pI Value

Organism Comment pI Value Maximum pI Value
Leishmania infantum calculated from amino acid sequence
-
 7.7