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Literature summary for 1.14.14.156 extracted from
- Cytochrome P450 CYP79B2 from Arabidopsis catalyzes the conversion of tryptophan to indole-3-acetaldoxime, a precursor of indole glucosinolates and indole-3-acetic acid (2000), J. Biol. Chem., 275, 33712-33717.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli C43(DE3) cells | Arabidopsis thaliana |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1-aminobenzotriazole | the activity of CYP79B2 is inhibited 18 and 75% by 0.4 mM and 4 mM 1-aminobenzotriazole | Arabidopsis thaliana |  |
| 2,4-Dichlorophenol | 800 mM 2,4-dichlorophenol added to the CYP79B2 reconstitution assay inhibits the activity by 72% | Arabidopsis thaliana | |
| H2O2 | 100 mM H2O2 added to the CYP79B2 reconstitution assay inhibits the activity by 96% | Arabidopsis thaliana | |
| MnCl2 | 1 mM MnCl2 added to the CYP79B2 reconstitution assay inhibits the activity by 34% | Arabidopsis thaliana | |
| additional information | CYP79B2 is not inhibited by Me2SO of a maximum concentration of 4% (v/v); the combination of 100 mM H2O2, 1 mM MnCl2, and 800 mM 2,4-dichlorophenol added to the reconstitution assay inhibits the CYP79B2 activity by 99% | Arabidopsis thaliana | |
| tetcyclasis | the activity of CYP79B2 is inhibited 54 and 76% by 0.02 and 0.2 mM tetcyclasis | Arabidopsis thaliana | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.021 | - |
L-tryptophan | in 100 mM Tricine, pH 7.9, at 34°C | Arabidopsis thaliana | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60000 | - |
SDS-PAGE | Arabidopsis thaliana |
| 61000 | - |
estimated from amino acid sequence | Arabidopsis thaliana |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | O81346 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| flower | - |
Arabidopsis thaliana | - |
| leaf | - |
Arabidopsis thaliana | - |
| root | highest expression in roots | Arabidopsis thaliana | - |
| stem | - |
Arabidopsis thaliana | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-tryptophan + 2 [reduced NADPH-hemoprotein reductase] + 2 O2 | - |
Arabidopsis thaliana | (E)-indol-3-ylacetaldoxime + 2 [oxidized NADPH-hemoprotein reductase] + CO2 + 3 H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CYP79B2 | - |
Arabidopsis thaliana |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Arabidopsis thaliana | |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Arabidopsis thaliana | CYP79B2 is wound-inducible | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | CYP79B2 is involved in indole glucosinolate biosynthesis. CYP79B2 functions in biosynthesis of indole-3-acetic acid | Arabidopsis thaliana |
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