BRENDA - Enzyme Database show
show all sequences of 1.14.14.156

Cytochrome P450 CYP79B2 from Arabidopsis catalyzes the conversion of tryptophan to indole-3-acetaldoxime, a precursor of indole glucosinolates and indole-3-acetic acid

Mikkelsen, M.; Hansen, C.; Wittstock, U.; Halkier, B.; J. Biol. Chem. 275, 33712-33717 (2000)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli C43(DE3) cells
Arabidopsis thaliana
Inhibitors
Inhibitors
Commentary
Organism
Structure
1-aminobenzotriazole
the activity of CYP79B2 is inhibited 18 and 75% by 0.4 mM and 4 mM 1-aminobenzotriazole
Arabidopsis thaliana
2,4-Dichlorophenol
800 mM 2,4-dichlorophenol added to the CYP79B2 reconstitution assay inhibits the activity by 72%
Arabidopsis thaliana
H2O2
100 mM H2O2 added to the CYP79B2 reconstitution assay inhibits the activity by 96%
Arabidopsis thaliana
MnCl2
1 mM MnCl2 added to the CYP79B2 reconstitution assay inhibits the activity by 34%
Arabidopsis thaliana
additional information
CYP79B2 is not inhibited by Me2SO of a maximum concentration of 4% (v/v); the combination of 100 mM H2O2, 1 mM MnCl2, and 800 mM 2,4-dichlorophenol added to the reconstitution assay inhibits the CYP79B2 activity by 99%
Arabidopsis thaliana
tetcyclasis
the activity of CYP79B2 is inhibited 54 and 76% by 0.02 and 0.2 mM tetcyclasis
Arabidopsis thaliana
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.021
-
L-tryptophan
in 100 mM Tricine, pH 7.9, at 34°C
Arabidopsis thaliana
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
60000
-
SDS-PAGE
Arabidopsis thaliana
61000
-
estimated from amino acid sequence
Arabidopsis thaliana
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arabidopsis thaliana
O81346
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
flower
-
Arabidopsis thaliana
-
leaf
-
Arabidopsis thaliana
-
root
highest expression in roots
Arabidopsis thaliana
-
stem
-
Arabidopsis thaliana
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-tryptophan + 2 [reduced NADPH-hemoprotein reductase] + 2 O2
-
715448
Arabidopsis thaliana
(E)-indol-3-ylacetaldoxime + 2 [oxidized NADPH-hemoprotein reductase] + CO2 + 3 H2O
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Arabidopsis thaliana
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli C43(DE3) cells
Arabidopsis thaliana
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Arabidopsis thaliana
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
1-aminobenzotriazole
the activity of CYP79B2 is inhibited 18 and 75% by 0.4 mM and 4 mM 1-aminobenzotriazole
Arabidopsis thaliana
2,4-Dichlorophenol
800 mM 2,4-dichlorophenol added to the CYP79B2 reconstitution assay inhibits the activity by 72%
Arabidopsis thaliana
H2O2
100 mM H2O2 added to the CYP79B2 reconstitution assay inhibits the activity by 96%
Arabidopsis thaliana
MnCl2
1 mM MnCl2 added to the CYP79B2 reconstitution assay inhibits the activity by 34%
Arabidopsis thaliana
additional information
CYP79B2 is not inhibited by Me2SO of a maximum concentration of 4% (v/v); the combination of 100 mM H2O2, 1 mM MnCl2, and 800 mM 2,4-dichlorophenol added to the reconstitution assay inhibits the CYP79B2 activity by 99%
Arabidopsis thaliana
tetcyclasis
the activity of CYP79B2 is inhibited 54 and 76% by 0.02 and 0.2 mM tetcyclasis
Arabidopsis thaliana
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.021
-
L-tryptophan
in 100 mM Tricine, pH 7.9, at 34°C
Arabidopsis thaliana
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
60000
-
SDS-PAGE
Arabidopsis thaliana
61000
-
estimated from amino acid sequence
Arabidopsis thaliana
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
flower
-
Arabidopsis thaliana
-
leaf
-
Arabidopsis thaliana
-
root
highest expression in roots
Arabidopsis thaliana
-
stem
-
Arabidopsis thaliana
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-tryptophan + 2 [reduced NADPH-hemoprotein reductase] + 2 O2
-
715448
Arabidopsis thaliana
(E)-indol-3-ylacetaldoxime + 2 [oxidized NADPH-hemoprotein reductase] + CO2 + 3 H2O
-
-
-
?
Expression
Organism
Commentary
Expression
Arabidopsis thaliana
CYP79B2 is wound-inducible
up
General Information
General Information
Commentary
Organism
metabolism
CYP79B2 is involved in indole glucosinolate biosynthesis. CYP79B2 functions in biosynthesis of indole-3-acetic acid
Arabidopsis thaliana
General Information (protein specific)
General Information
Commentary
Organism
metabolism
CYP79B2 is involved in indole glucosinolate biosynthesis. CYP79B2 functions in biosynthesis of indole-3-acetic acid
Arabidopsis thaliana
Expression (protein specific)
Organism
Commentary
Expression
Arabidopsis thaliana
CYP79B2 is wound-inducible
up
Other publictions for EC 1.14.14.156
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
716536
Sanchez-Vallet
Tryptophan-derived secondary m ...
Arabidopsis thaliana
Plant J.
63
115-127
2010
-
-
-
-
-
-
-
-
-
-
-
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6
-
-
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-
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1
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1
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1
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-
1
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-
-
-
-
-
1
1
-
-
-
714117
Glawischnig
The role of cytochrome P450 en ...
Arabidopsis thaliana
Biochem. Soc. Trans.
34
1206-1208
2006
-
-
-
-
-
-
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1
-
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4
-
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1
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1
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1
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1
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1
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1
1
-
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-
716733
Glawischnig
Camalexin is synthesized from ...
Arabidopsis thaliana
Proc. Natl. Acad. Sci. USA
101
8245-8250
2004
-
-
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-
-
-
-
-
-
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3
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1
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1
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1
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1
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1
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1
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-
1
3
3
1
-
-
713941
Naur
CYP79B1 from Sinapis alba conv ...
Sinapis alba
Arch. Biochem. Biophys.
409
235-241
2003
-
-
1
-
-
-
-
1
-
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5
-
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2
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1
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1
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1
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1
1
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1
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2
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1
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1
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716592
Mikkelsen
Modulation of CYP79 genes and ...
Arabidopsis thaliana
Plant Physiol.
131
298-308
2003
-
-
-
-
-
-
-
-
-
-
-
-
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3
-
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1
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1
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-
1
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1
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1
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1
-
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-
-
-
-
-
2
-
-
2
-
-
715111
Zhao
Trp-dependent auxin biosynthes ...
Arabidopsis thaliana
Genes Dev.
16
3100-3112
2002
-
-
-
-
-
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-
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-
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3
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1
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1
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1
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-
1
-
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-
-
-
-
-
-
-
-
2
2
-
-
-
715448
Mikkelsen
Cytochrome P450 CYP79B2 from A ...
Arabidopsis thaliana
J. Biol. Chem.
275
33712-33717
2000
-
-
1
-
-
-
6
1
-
-
2
-
-
4
-
-
-
-
-
4
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
6
-
1
-
-
2
-
-
-
-
-
-
4
-
-
1
-
-
-
-
-
-
-
-
-
1
1
1
1
-
-
716797
Hull
Arabidopsis cytochrome P450s t ...
Arabidopsis thaliana
Proc. Natl. Acad. Sci. USA
97
2379-2384
2000
-
-
1
-
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2
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2
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1
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2
2
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2
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1
1
2
1
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