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show all sequences of 1.14.14.157

Cytochrome P450 monooxygenases of DIBOA biosynthesis: Specificity and conservation among grasses

Glawischnig, E.; Grün, S.; Frey, M.; Gierl, A.; Phytochemistry 50, 925-930 (1999)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Saccharomyces cerevisiae strain WAT11 and in Escherichia coli strain BL21 (DE3)
Zea mays
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.01
-
indolin-2-one
recombinant enzyme, in 50 mM potassium phosphate, pH 7.5, at 25°C
Zea mays
0.011
-
benzoxazin-3-one
recombinant enzyme, in 50 mM potassium phosphate, pH 7.5, at 25°C
Zea mays
0.017
-
indolin-2-one
native enzyme, in 50 mM potassium phosphate, pH 7.5, at 25°C
Zea mays
0.018
-
benzoxazin-3-one
native enzyme, in 50 mM potassium phosphate, pH 7.5, at 25°C
Zea mays
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Zea mays
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Zea mays
-
hybrid Blizzard
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.00194
-
recombinant enzyme, using indolin-2-one as substrate, at pH 7.5 and 25°C
Zea mays
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
benzoxazin-3-one + [reduced NADPH-hemoprotein reductase] + O2
-
723355
Zea mays
2-hydroxy-1,4-benzoxazin-3-one + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
indolin-2-one + [reduced NADPH-hemoprotein reductase] + O2
-
723355
Zea mays
3-hydroxyindolin-2-one + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Zea mays
NADPH
-
Zea mays
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Saccharomyces cerevisiae strain WAT11 and in Escherichia coli strain BL21 (DE3)
Zea mays
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Zea mays
NADPH
-
Zea mays
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.01
-
indolin-2-one
recombinant enzyme, in 50 mM potassium phosphate, pH 7.5, at 25°C
Zea mays
0.011
-
benzoxazin-3-one
recombinant enzyme, in 50 mM potassium phosphate, pH 7.5, at 25°C
Zea mays
0.017
-
indolin-2-one
native enzyme, in 50 mM potassium phosphate, pH 7.5, at 25°C
Zea mays
0.018
-
benzoxazin-3-one
native enzyme, in 50 mM potassium phosphate, pH 7.5, at 25°C
Zea mays
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Zea mays
-
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.00194
-
recombinant enzyme, using indolin-2-one as substrate, at pH 7.5 and 25°C
Zea mays
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
benzoxazin-3-one + [reduced NADPH-hemoprotein reductase] + O2
-
723355
Zea mays
2-hydroxy-1,4-benzoxazin-3-one + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
indolin-2-one + [reduced NADPH-hemoprotein reductase] + O2
-
723355
Zea mays
3-hydroxyindolin-2-one + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Other publictions for EC 1.14.14.157
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
723355
Glawischnig
Cytochrome P450 monooxygenases ...
Zea mays
Phytochemistry
50
925-930
1999
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1
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4
1
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1
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4
1
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1
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2
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670884
Frey
Analysis of a chemical plant d ...
Zea mays
Science
277
696-699
1997
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1
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1
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1
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1
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2
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1
2
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1
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1
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