BRENDA - Enzyme Database show
show all sequences of 1.14.14.158

Over-expression of Arabidopsis thaliana carotenoid hydroxylases individually and in combination with a beta-carotene ketolase provides insight into in vivo functions

Kim, J.; Cheng, K.; Craft, N.; Hamberger, B.; Douglas, C.; Phytochemistry 71, 168-178 (2010)

Data extracted from this reference:

Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arabidopsis thaliana
-
ecotype Col-0
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
alpha-carotene + [reduced NADPH-hemoprotein reductase] + O2
CYP97C1 has alpha-carotene epsilon-ring hydroxylase activity
716454
Arabidopsis thaliana
alpha-cryptoxanthin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
zeinoxanthin + [reduced NADPH-hemoprotein reductase] + O2
-
716454
Arabidopsis thaliana
lutein + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
heme
-
Arabidopsis thaliana
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
heme
-
Arabidopsis thaliana
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
alpha-carotene + [reduced NADPH-hemoprotein reductase] + O2
CYP97C1 has alpha-carotene epsilon-ring hydroxylase activity
716454
Arabidopsis thaliana
alpha-cryptoxanthin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
zeinoxanthin + [reduced NADPH-hemoprotein reductase] + O2
-
716454
Arabidopsis thaliana
lutein + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
General Information
General Information
Commentary
Organism
malfunction
mis-expressing of CYP97C1 impacts carotenoids metabolic flux
Arabidopsis thaliana
General Information (protein specific)
General Information
Commentary
Organism
malfunction
mis-expressing of CYP97C1 impacts carotenoids metabolic flux
Arabidopsis thaliana
Other publictions for EC 1.14.14.158
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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728502
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Solanum lycopersicum
Plant Physiol. Biochem.
70
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-
1
-
1
-
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1
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1
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5
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2
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1
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1
2
2
1
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727255
Fiore
A quadruple mutant of Arabidop ...
Arabidopsis thaliana
BMC Plant Biol.
12
50
2012
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-
1
-
1
-
-
-
-
1
-
6
-
3
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1
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6
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2
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2
4
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2
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2
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6
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2
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6
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3
6
-
-
-
716454
Kim
Over-expression of Arabidopsis ...
Arabidopsis thaliana
Phytochemistry
71
168-178
2010
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1
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716737
Kim
Defining the primary route for ...
Arabidopsis thaliana
Proc. Natl. Acad. Sci. USA
103
3474-3479
2006
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716732
Tian
The Arabidopsis LUT1 locus enc ...
Arabidopsis thaliana
Proc. Natl. Acad. Sci. USA
101
402-407
2004
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1
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716526
Pogson
Arabidopsis carotenoid mutants ...
Arabidopsis thaliana
Plant Cell
8
1627-1639
1996
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