BRENDA - Enzyme Database show
show all sequences of 1.14.14.158

A quadruple mutant of Arabidopsis reveals a beta-carotene hydroxylation activity for LUT1/CYP97C1 and a regulatory role of xanthophylls on determination of the PSI/PSII ratio

Fiore, A.; DallOsto, L.; Cazzaniga, S.; Diretto, G.; Giuliano, G.; Bassi, R.; BMC Plant Biol. 12, 50 (2012)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
genes lut1 and lut5, real-time PCR expression analysis; genes lut1 and lut5, real-time PCR expression analysis
Arabidopsis thaliana
Engineering
Amino acid exchange
Commentary
Organism
additional information
construction of the triple chy1chy2lut5 mutant, which is almost completely depleted in beta-xanthophylls. Quadruple chy1chy2lut2lut5 mutant, additionally carrying the lut2 mutation (affecting lycopene epsilon-cyclase) lacks lutein and shows a compensatory increase in beta-xanthophylls with respect to chy1chy2lut5 mutant. Leaf carotenoid contents of wild-type and mutant enzymes, overview; construction of the triple chy1chy2lut5 mutant, which is almost completely depleted in beta-xanthophylls. Quadruple chy1chy2lut2lut5 mutant, additionally carrying the lut2 mutation (affecting lycopene epsilon-cyclase) lacks lutein and shows a compensatory increase in beta-xanthophylls with respect to chy1chy2lut5 mutant. Leaf carotenoid contents of wild-type and mutant enzymes, overview
Arabidopsis thaliana
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe2+
a heme-containing cytochrome P450 enzyme; a heme-containing cytochrome P450 enzyme
Arabidopsis thaliana
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
alpha-carotene + [reduced NADPH-hemoprotein reductase] + O2
Arabidopsis thaliana
-
lutein + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
beta-carotene + reduced cytochrome P-450 + O2
Arabidopsis thaliana
-
zeaxanthin + oxidized cytochrome P-450 + H2O
-
-
?
additional information
Arabidopsis thaliana
LUT1/CYP97C1 protein reveals a major beta-carotene hydroxylase activity in vivo when depleted in its preferred substrate alpha-carotene
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arabidopsis thaliana
Q6TBX7
gene lut1
-
Arabidopsis thaliana
Q93VK5
gene lut5
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
;
Arabidopsis thaliana
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
alpha-carotene + [reduced NADPH-hemoprotein reductase] + O2
-
727255
Arabidopsis thaliana
lutein + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
beta-carotene + reduced cytochrome P-450 + O2
-
727255
Arabidopsis thaliana
zeaxanthin + oxidized cytochrome P-450 + H2O
-
-
-
?
additional information
LUT1/CYP97C1 protein reveals a major beta-carotene hydroxylase activity in vivo when depleted in its preferred substrate alpha-carotene
727255
Arabidopsis thaliana
?
-
-
-
-
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P450
;
Arabidopsis thaliana
heme
;
Arabidopsis thaliana
Cloned(Commentary) (protein specific)
Commentary
Organism
genes lut1 and lut5, real-time PCR expression analysis
Arabidopsis thaliana
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Arabidopsis thaliana
heme
-
Arabidopsis thaliana
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
construction of the triple chy1chy2lut5 mutant, which is almost completely depleted in beta-xanthophylls. Quadruple chy1chy2lut2lut5 mutant, additionally carrying the lut2 mutation (affecting lycopene epsilon-cyclase) lacks lutein and shows a compensatory increase in beta-xanthophylls with respect to chy1chy2lut5 mutant. Leaf carotenoid contents of wild-type and mutant enzymes, overview
Arabidopsis thaliana
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe2+
a heme-containing cytochrome P450 enzyme
Arabidopsis thaliana
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
alpha-carotene + [reduced NADPH-hemoprotein reductase] + O2
Arabidopsis thaliana
-
lutein + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
beta-carotene + reduced cytochrome P-450 + O2
Arabidopsis thaliana
-
zeaxanthin + oxidized cytochrome P-450 + H2O
-
-
?
additional information
Arabidopsis thaliana
LUT1/CYP97C1 protein reveals a major beta-carotene hydroxylase activity in vivo when depleted in its preferred substrate alpha-carotene
?
-
-
-
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
-
Arabidopsis thaliana
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
alpha-carotene + [reduced NADPH-hemoprotein reductase] + O2
-
727255
Arabidopsis thaliana
lutein + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
beta-carotene + reduced cytochrome P-450 + O2
-
727255
Arabidopsis thaliana
zeaxanthin + oxidized cytochrome P-450 + H2O
-
-
-
?
additional information
LUT1/CYP97C1 protein reveals a major beta-carotene hydroxylase activity in vivo when depleted in its preferred substrate alpha-carotene
727255
Arabidopsis thaliana
?
-
-
-
-
General Information
General Information
Commentary
Organism
malfunction
quadruple chy1chy2lut2lut5 mutant lacks lutein and shows a compensatory increase in beta-xanthophylls with respect to the chy1chy2lut5 mutant. Chy1chy2lut2lut5 mutant plants show an even stronger photosensitivity than mutant chy1chy2lut5, a complete lack of qE, the rapidly reversible component of non-photochemical quenching, and a peculiar organization of the pigment binding complexes into thylakoids. The chy1chy2lut2lut5 mutant is depleted in Lhcb subunits and is specifically affected in Photosystem I function, showing a deficiency in PSI-LHCI supercomplexes, phenotype, overview; quadruple chy1chy2lut2lut5 mutant lacks lutein and shows a compensatory increase in beta-xanthophylls with respect to the chy1chy2lut5 mutant. Chy1chy2lut2lut5 mutant plants show an even stronger photosensitivity than mutant chy1chy2lut5, a complete lack of qE, the rapidly reversible component of non-photochemical quenching, and a peculiar organization of the pigment binding complexes into thylakoids. The chy1chy2lut2lut5 mutant is depleted in Lhcb subunits and is specifically affected in Photosystem I function, showing a deficiency in PSI-LHCI supercomplexes, phenotype, overview
Arabidopsis thaliana
metabolism
the first step in xanthophyll biosynthesis from alpha- and beta-carotene is the hydroxylation of epsilon- and beta-rings, performed by both non-heme iron oxygenases CHY1 and CHY2, and by P450 cytochromes, LUT1/CYP97C1 and LUT5/CYP97A3. CHY1, CHY2, LUT1/CYP97C1 and LUT5/CYP97A3 are the complete complement of carotene hydroxylases in Arabidopsis thaliana; the first step in xanthophyll biosynthesis from alpha- and beta-carotene is the hydroxylation of epsilon- and beta-rings, performed by both non-heme iron oxygenases CHY1 and CHY2, and by P450 cytochromes, LUT1/CYP97C1 and LUT5/CYP97A3. CHY1, CHY2, LUT1/CYP97C1 and LUT5/CYP97A3 are the complete complement of carotene hydroxylases in Arabidopsis thaliana
Arabidopsis thaliana
physiological function
the enzyme is involved in xanthophyll biosynthesis, correlation between xanthophyll levels and the PSI-PSII ratio, xanthophylls are needed for normal level of Photosystem I and LHCII accumulation; the enzyme is involved in xanthophyll biosynthesis, correlation between xanthophyll levels and the PSI-PSII ratio, xanthophylls are needed for normal level of Photosystem I and LHCII accumulation
Arabidopsis thaliana
General Information (protein specific)
General Information
Commentary
Organism
malfunction
quadruple chy1chy2lut2lut5 mutant lacks lutein and shows a compensatory increase in beta-xanthophylls with respect to the chy1chy2lut5 mutant. Chy1chy2lut2lut5 mutant plants show an even stronger photosensitivity than mutant chy1chy2lut5, a complete lack of qE, the rapidly reversible component of non-photochemical quenching, and a peculiar organization of the pigment binding complexes into thylakoids. The chy1chy2lut2lut5 mutant is depleted in Lhcb subunits and is specifically affected in Photosystem I function, showing a deficiency in PSI-LHCI supercomplexes, phenotype, overview
Arabidopsis thaliana
metabolism
the first step in xanthophyll biosynthesis from alpha- and beta-carotene is the hydroxylation of epsilon- and beta-rings, performed by both non-heme iron oxygenases CHY1 and CHY2, and by P450 cytochromes, LUT1/CYP97C1 and LUT5/CYP97A3. CHY1, CHY2, LUT1/CYP97C1 and LUT5/CYP97A3 are the complete complement of carotene hydroxylases in Arabidopsis thaliana
Arabidopsis thaliana
physiological function
the enzyme is involved in xanthophyll biosynthesis, correlation between xanthophyll levels and the PSI-PSII ratio, xanthophylls are needed for normal level of Photosystem I and LHCII accumulation
Arabidopsis thaliana
Other publictions for EC 1.14.14.158
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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6
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6
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