Cloned (Comment) | Organism |
---|---|
gene CitCYP97C, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis. When CitHYb and CitCYP97C are coexpressed in Escherichia coli strain BL21(DE3) the monohydroxylated zeinoxanthin, which is produced by CitHYb, is further converted to lutein by CitCYP97C, when CitCYP97A and CitCYP97C are coexpressed or CitCYP97B and CitCYP97C are coexpressed, no hydroxylated carotene is detected in the alpha-carotene- and beta-carotene-accumulating Escherichia coli strain BL21(DE3) cells | Citrus unshiu |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | as most carotenoids are synthesized and stored in plastids, the location of CitCYP97C, together with the other enzymes involved, i.e. CitHYb, CitCYP97A, and CitCYP97B, within plastid allows them to catalyze the reaction of carotene hydroxylation | Citrus unshiu | 9507 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
zeinoxanthin + [reduced NADPH-hemoprotein reductase] + O2 | Citrus unshiu | - |
lutein + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Citrus unshiu | A0A1L7NT18 | - |
- |
Purification (Comment) | Organism |
---|---|
partially, isolation of CitCYP79C from fruits | Citrus unshiu |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit | in flavedo and juice sacs, expression of CitCYP97C increases with two peaks in September and November, respectively | Citrus unshiu | - |
additional information | lutein content in the juice sacs during the ripening process is high during November and December, and in the flavedo it is highest in August, while lowest in December, overview | Citrus unshiu | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
zeinoxanthin + [reduced NADPH-hemoprotein reductase] + O2 | - |
Citrus unshiu | lutein + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
zeinoxanthin + [reduced NADPH-hemoprotein reductase] + O2 | recombinant enzyme | Citrus unshiu | lutein + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 61600, about, sequence calculation of CitCYP97C | Citrus unshiu |
Synonyms | Comment | Organism |
---|---|---|
CitCYP97C | - |
Citrus unshiu |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome P450 | - |
Citrus unshiu | |
heme | - |
Citrus unshiu |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved in the carotenoid biosynthesis pathway | Citrus unshiu |
physiological function | of the four citrus carotene hydroxylases presented in four distinct clusters, CitCYP97C is the one responsible for epsilon-ring hydroxylation in Citrus unshiu, while CitCYP97A and CitCYP97B hydroxylate the alpha- and beta-rings of alpha-carotene, respectively, roles of four carotene hydroxylase genes (CitHYb, CitCYP97A, CitCYP97B, and CitCYP97C) in regulating xanthophylls biosynthesis. Zeaxanthin increases significantly during the ripening process in Citrus fruits, contents of alpha-carotene and lutein increase gradually in the juice sacs during the ripening process, the content of beta-cryptoxanthin, the major carotenoid in Satsuma mandarin, increases significantly during the ripening process in December, overview | Citrus unshiu |