BRENDA - Enzyme Database show
show all sequences of 1.14.14.158

Cloning and functional characterization of the maize (Zea mays L.) carotenoid epsilon hydroxylase gene

Chang, S.; Berman, J.; Sheng, Y.; Wang, Y.; Capell, T.; Shi, L.; Ni, X.; Sandmann, G.; Christou, P.; Zhu, C.; PLoS ONE 10, e0128758 (2015)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression of CYP97C19 cDNA under the control of the constitutive CaMV 35S promoter in the Arabidopsis thaliana lut1 knockout mutant, which lacks a functional CYP97C1 (LUT1) gene; gene CYP97C19, cDNA is cloned from leaves, the ZmCYP97C19 gene has nine introns and ten exons, DNA and amino acid sequence determination and analysis, sequence comparisons, genetic structure, overview. Functional recombinant expression under the control of constitutive CaMV 35S promoter in rosettes leaves of the Arabidopsis thaliana lut1 knockout mutant, which lacks a functional CYP97C1 (LUT1) gene. Lutein accumulates to high levels in the rosette leaves of the transgenic lines but not in the untransformed lut1 mutants, carotenoid extraction and quantification from Arabidopsis thaliana lut1-CYP97C1 leaves
Zea mays
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
zeinoxanthin + [reduced NADPH-hemoprotein reductase] + O2
Zea mays
maize CYP97C19 is an enzyme with strong zeinoxanthin epsilon-ring hydroxylation activity
lutein + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
zeinoxanthin + [reduced NADPH-hemoprotein reductase] + O2
Zea mays L. cv B73
maize CYP97C19 is an enzyme with strong zeinoxanthin epsilon-ring hydroxylation activity
lutein + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Zea mays
K9J9Z8
; cv. B73
-
Zea mays L. cv B73
K9J9Z8
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
endosperm
-
Zea mays
-
leaf
-
Zea mays
-
rosette leaf
-
Zea mays
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
zeinoxanthin + [reduced NADPH-hemoprotein reductase] + O2
maize CYP97C19 is an enzyme with strong zeinoxanthin epsilon-ring hydroxylation activity
746186
Zea mays
lutein + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
zeinoxanthin + [reduced NADPH-hemoprotein reductase] + O2
maize CYP97C19 is an enzyme with strong zeinoxanthin epsilon-ring hydroxylation activity
746186
Zea mays L. cv B73
lutein + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 61900, about, sequence calculation of ZmCYP97C19
Zea mays
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Zea mays
heme
-
Zea mays
Cloned(Commentary) (protein specific)
Commentary
Organism
expression of CYP97C19 cDNA under the control of the constitutive CaMV 35S promoter in the Arabidopsis thaliana lut1 knockout mutant, which lacks a functional CYP97C1 (LUT1) gene; gene CYP97C19, cDNA is cloned from leaves, the ZmCYP97C19 gene has nine introns and ten exons, DNA and amino acid sequence determination and analysis, sequence comparisons, genetic structure, overview. Functional recombinant expression under the control of constitutive CaMV 35S promoter in rosettes leaves of the Arabidopsis thaliana lut1 knockout mutant, which lacks a functional CYP97C1 (LUT1) gene. Lutein accumulates to high levels in the rosette leaves of the transgenic lines but not in the untransformed lut1 mutants, carotenoid extraction and quantification from Arabidopsis thaliana lut1-CYP97C1 leaves
Zea mays
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Zea mays
heme
-
Zea mays
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
zeinoxanthin + [reduced NADPH-hemoprotein reductase] + O2
Zea mays
maize CYP97C19 is an enzyme with strong zeinoxanthin epsilon-ring hydroxylation activity
lutein + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
zeinoxanthin + [reduced NADPH-hemoprotein reductase] + O2
Zea mays L. cv B73
maize CYP97C19 is an enzyme with strong zeinoxanthin epsilon-ring hydroxylation activity
lutein + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
endosperm
-
Zea mays
-
leaf
-
Zea mays
-
rosette leaf
-
Zea mays
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
zeinoxanthin + [reduced NADPH-hemoprotein reductase] + O2
maize CYP97C19 is an enzyme with strong zeinoxanthin epsilon-ring hydroxylation activity
746186
Zea mays
lutein + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
zeinoxanthin + [reduced NADPH-hemoprotein reductase] + O2
maize CYP97C19 is an enzyme with strong zeinoxanthin epsilon-ring hydroxylation activity
746186
Zea mays L. cv B73
lutein + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 61900, about, sequence calculation of ZmCYP97C19
Zea mays
Other publictions for EC 1.14.14.158
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
745823
Reddy
Molecular cloning and charact ...
Ixeridium dentatum
Molecules
22
E1449
2017
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
3
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
3
-
-
1
1
-
-
-
-
-
-
-
-
-
2
2
-
-
-
744606
Ma
Expression and functional ana ...
Citrus unshiu
BMC Plant Biol.
16
148
2016
-
-
1
-
-
-
-
-
1
-
-
1
-
5
-
-
1
-
-
2
-
-
2
1
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
-
1
-
-
1
-
-
-
1
-
2
-
-
2
1
-
-
-
-
-
-
-
-
-
2
2
-
-
-
746186
Chang
Cloning and functional charac ...
Zea mays, Zea mays L. cv B73
PLoS ONE
10
e0128758
2015
-
-
1
-
-
-
-
-
-
-
-
2
-
5
-
-
-
-
-
3
-
-
2
1
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
3
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
749030
Takemura
Biosynthetic routes of hydrox ...
Marchantia polymorpha
Planta
241
699-710
2015
-
-
1
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
728502
Zhou
Overexpression of a tomato car ...
Solanum lycopersicum
Plant Physiol. Biochem.
70
235-245
2013
-
-
1
-
1
-
-
-
1
-
-
1
-
5
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
1
2
2
1
-
-
727255
Fiore
A quadruple mutant of Arabidop ...
Arabidopsis thaliana
BMC Plant Biol.
12
50
2012
-
-
1
-
1
-
-
-
-
1
-
6
-
3
-
-
-
-
-
1
-
-
6
-
-
-
-
-
-
-
-
2
-
-
-
-
-
2
4
-
2
-
-
-
-
-
-
2
-
6
-
-
-
-
-
2
-
-
6
-
-
-
-
-
-
-
-
-
-
3
6
-
-
-
716454
Kim
Over-expression of Arabidopsis ...
Arabidopsis thaliana
Phytochemistry
71
168-178
2010
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
716737
Kim
Defining the primary route for ...
Arabidopsis thaliana
Proc. Natl. Acad. Sci. USA
103
3474-3479
2006
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
716732
Tian
The Arabidopsis LUT1 locus enc ...
Arabidopsis thaliana
Proc. Natl. Acad. Sci. USA
101
402-407
2004
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
716526
Pogson
Arabidopsis carotenoid mutants ...
Arabidopsis thaliana
Plant Cell
8
1627-1639
1996
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-