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Literature summary for 1.14.14.16 extracted from
- Challenges of steroid biotransformation with human cytochrome P450 monooxygenase CYP21 using resting cells of recombinant Schizosaccharomyces pombe (2010), J. Biotechnol., 146, 179-185.
Application
| Application | Comment | Organism |
|---|---|---|
| biotechnology | the CYP21 expression model system using resting Schizosaccharomyces pombe cells can be used for biotransformations | Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene CYP21, expression in Schizosaccharomyces pombe strain CAD18, method optimization and evaluation, and quantification of the intracellular redox cofactor pool in transformed cells, overview | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 17alpha-hydroxyprogesterone | inhibits above 0.2mM | Homo sapiens |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | reaction kinetic, overview | Homo sapiens |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | a cytochrome P450 monooxygenase | Homo sapiens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 17-hydroxyprogesterone + NADPH + H+ + O2 | Homo sapiens | - |
11-deoxycortisol + NADP+ + H2O | - |
? | |
| additional information | Homo sapiens | CYP21 performs the regio- and stereo-selective 21-hydroxylation of 17-alpha-hydroxyprogesterone when recombinantly expressed in Schizosaccharomyces pombe | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | P08686 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 17-hydroxyprogesterone + NADPH + H+ + O2 | - |
Homo sapiens | 11-deoxycortisol + NADP+ + H2O | - |
? | |
| 17-hydroxyprogesterone + NADPH + H+ + O2 | the redox cofactor provides the electrons required for a P450-dependent hydroxylation. Maximal CYP 21 activity is observed in presence of low substrate concentrations of 0.2 mM. Higher substrate concentrations reduce the activity and inhibit the enzyme | Homo sapiens | 11-deoxycortisol + NADP+ + H2O | - |
? | |
| additional information | CYP21 performs the regio- and stereo-selective 21-hydroxylation of 17-alpha-hydroxyprogesterone when recombinantly expressed in Schizosaccharomyces pombe | Homo sapiens | ? | - |
? | |
| additional information | reaction method optimization, overview | Homo sapiens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CYP21 | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| cytochrome P450 | Cyp21 is a cytochrome P450 monooxygenase | Homo sapiens | |
| NADPH | the redox cofactor provides the electrons required for a P450-dependent hydroxylation | Homo sapiens | |
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Release 2023.1 (January 2023)
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