BRENDA - Enzyme Database show
show all sequences of 1.14.14.16

Minor activities and transition state properties of the human steroid hydroxylases cytochromes P450c17 and P450c21, from reactions observed with deuterium-labeled substrates

Yoshimoto, F.K.; Zhou, Y.; Peng, H.M.; Stidd, D.; Yoshimoto, J.A.; Sharma, K.K.; Matthew, S.; Auchus, R.J.; Biochemistry 51, 7064-7077 (2012)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression of wild-type and mutant enzymes in yeast microsomes
Homo sapiens
Engineering
Amino acid exchange
Commentary
Organism
V359A
site-directed mutagenesis, the mutant shows altered product formation compared to the wild-type enzyme
Homo sapiens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
calculation of intramolecular and intermolecular kinetic isotope effects for wild-type and mutant enzymes with substrates 21-[2H]-progesterone, 21,21,21-[2H3]-progesterone, and 21,21,21-[2H3]-17-hydroxyprogesterone, overview
Homo sapiens
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Homo sapiens
-
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
17alpha-hydroxyprogesterone + [reduced NADPH-hemoprotein reductase] + O2
Homo sapiens
-
11-deoxycortisol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
progesterone + [reduced NADPH-hemoprotein reductase] + O2
Homo sapiens
-
11-deoxycorticosterone + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
-
-
-
Purification (Commentary)
Commentary
Organism
recombinant wild-type and mutant enzymes from yeast microsomes
Homo sapiens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
17alpha-hydroxyprogesterone + [reduced NADPH-hemoprotein reductase] + O2
-
726997
Homo sapiens
11-deoxycortisol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
additional information
human CYP21A2 also has progesterone 16alpha-hydroxylase activity. Product analysis by LC-MS/MS
726997
Homo sapiens
?
-
-
-
-
progesterone + [reduced NADPH-hemoprotein reductase] + O2
-
726997
Homo sapiens
11-deoxycorticosterone + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
progesterone + [reduced NADPH-hemoprotein reductase] + O2
mutant V359A produces an additional metabolite, 16alpha-hydroxyprogesterone of progesterone, very low activity with the wild-type enzyme
726997
Homo sapiens
16alpha-hydroxy-progesterone + [oxidized NADPH-hemoprotein reductase] + H2O
product identification by TLC
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Homo sapiens
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
assay at
Homo sapiens
Cloned(Commentary) (protein specific)
Commentary
Organism
expression of wild-type and mutant enzymes in yeast microsomes
Homo sapiens
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
V359A
site-directed mutagenesis, the mutant shows altered product formation compared to the wild-type enzyme
Homo sapiens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
calculation of intramolecular and intermolecular kinetic isotope effects for wild-type and mutant enzymes with substrates 21-[2H]-progesterone, 21,21,21-[2H3]-progesterone, and 21,21,21-[2H3]-17-hydroxyprogesterone, overview
Homo sapiens
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Homo sapiens
-
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
17alpha-hydroxyprogesterone + [reduced NADPH-hemoprotein reductase] + O2
Homo sapiens
-
11-deoxycortisol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
progesterone + [reduced NADPH-hemoprotein reductase] + O2
Homo sapiens
-
11-deoxycorticosterone + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant wild-type and mutant enzymes from yeast microsomes
Homo sapiens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
17alpha-hydroxyprogesterone + [reduced NADPH-hemoprotein reductase] + O2
-
726997
Homo sapiens
11-deoxycortisol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
additional information
human CYP21A2 also has progesterone 16alpha-hydroxylase activity. Product analysis by LC-MS/MS
726997
Homo sapiens
?
-
-
-
-
progesterone + [reduced NADPH-hemoprotein reductase] + O2
-
726997
Homo sapiens
11-deoxycorticosterone + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
progesterone + [reduced NADPH-hemoprotein reductase] + O2
mutant V359A produces an additional metabolite, 16alpha-hydroxyprogesterone of progesterone, very low activity with the wild-type enzyme
726997
Homo sapiens
16alpha-hydroxy-progesterone + [oxidized NADPH-hemoprotein reductase] + H2O
product identification by TLC
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Homo sapiens
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
assay at
Homo sapiens
Other publictions for EC 1.14.14.16
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
745363
Wang
Functional analysis of human ...
Homo sapiens
J. Biol. Chem.
292
10767-10778
2017
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1
12
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1
24
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25
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1
12
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24
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1
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3
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25
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24
24
734302
Pallan
Human cytochrome P450 21A2, th ...
Bos taurus, Homo sapiens
J. Biol. Chem.
290
13128-13143
2015
-
-
2
1
-
-
-
4
-
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1
4
-
4
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2
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3
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4
1
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4
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-
-
-
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2
-
1
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4
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1
4
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2
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3
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4
1
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4
-
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-
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-
2
2
-
4
4
745776
Pallan
Research Resource Correlating ...
Homo sapiens
Mol. Endocrinol.
29
1375-1384
2015
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1
-
1
2
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-
6
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2
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1
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6
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1
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1
2
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6
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1
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2
-
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-
-
6
-
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-
6
6
735122
Doleschall
Common genetic variants of the ...
Homo sapiens
PLoS ONE
9
e107244
2014
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-
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-
1
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2
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2
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1
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1
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1
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2
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744331
Yoshimoto
Epoxidation activities of hum ...
Homo sapiens
Biochemistry
53
7531-7540
2014
-
-
-
-
1
-
-
-
-
-
-
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2
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1
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1
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-
1
-
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-
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728691
Haider
Structure-phenotype correlatio ...
Homo sapiens
Proc. Natl. Acad. Sci. USA
110
2605-2610
2013
-
-
-
-
75
-
-
-
1
1
-
-
-
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
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-
1
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-
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1
-
75
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1
1
-
-
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-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
726923
Sushko
Mechanism of intermolecular in ...
Homo sapiens
Biochemistry (Moscow)
77
585-592
2012
-
-
1
-
-
-
-
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1
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2
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2
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1
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2
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1
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1
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1
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1
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2
-
1
-
-
-
1
-
-
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-
1
1
-
-
-
726997
Yoshimoto
Minor activities and transitio ...
Homo sapiens
Biochemistry
51
7064-7077
2012
-
-
1
-
1
-
-
1
1
-
-
2
-
2
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-
1
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4
-
1
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1
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1
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1
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1
1
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2
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1
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-
4
-
1
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1
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-
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-
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-
727898
Zhao
Three-dimensional structure of ...
Bos taurus, Homo sapiens
J. Biol. Chem.
287
10613-10622
2012
-
-
1
1
2
-
-
3
-
1
-
4
-
3
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1
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5
1
1
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1
1
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1
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1
1
1
2
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3
-
1
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4
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1
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-
5
1
1
-
-
1
1
-
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-
-
2
2
-
-
-
728213
Yoshimoto
Synthesis of halogenated pregn ...
Homo sapiens
J. Steroid Biochem. Mol. Biol.
128
38-50
2012
-
-
1
-
-
-
1
-
1
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2
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3
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4
-
1
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1
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1
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1
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1
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4
-
1
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-
1
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2
2
-
-
-
728828
Parr
Unexpected contribution of cyt ...
Homo sapiens
Toxicol. Lett.
213
381-391
2012
-
-
-
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-
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1
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1
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2
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1
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3
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-
716052
Gaffney
Functional characterisation of ...
Homo sapiens
J. Steroid Biochem. Mol. Biol.
123
109-114
2011
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1
-
3
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4
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2
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4
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1
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3
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4
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4
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-
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1
1
-
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-
726962
Mizrachi
Why human cytochrome P450c21 i ...
Homo sapiens
Biochemistry
50
3968-3974
2011
-
-
-
-
9
-
1
9
-
1
-
1
-
2
-
-
-
-
-
-
-
-
4
-
1
-
-
-
1
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-
1
-
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-
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-
-
1
-
9
-
-
1
-
9
-
1
-
1
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-
-
-
-
-
-
-
4
-
1
-
-
-
1
-
-
-
-
2
2
-
-
-
697794
Marques
Mutational characterization of ...
Homo sapiens
Exp. Clin. Endocrinol. Diabetes
118
505-512
2010
-
-
-
-
6
-
-
-
-
-
-
-
-
2
-
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1
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6
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1
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715325
Rottembourg
21-Hydroxylase epitopes are ta ...
Homo sapiens
J. Autoimmun.
35
309-315
2010
-
-
-
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2
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1
1
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-
715690
Zehentgruber
Challenges of steroid biotrans ...
Homo sapiens
J. Biotechnol.
146
179-185
2010
-
1
1
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-
-
1
1
-
1
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2
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4
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2
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4
-
1
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1
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2
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1
1
2
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1
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1
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1
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2
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2
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4
-
1
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1
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-
696981
Concolino
A new CYP21A1P/CYP21A2 chimeri ...
Homo sapiens
BMC Med. Genet.
10
72
2009
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1
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2
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-
698190
Bleicken
Functional characterization of ...
Homo sapiens
Hum. Mutat.
30
E443-E450
2009
-
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1
-
3
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5
-
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3
-
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2
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1
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1
1
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3
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5
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2
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-
698536
Bratland
The purification and applicati ...
Homo sapiens
J. Autoimmun.
33
58-67
2009
-
-
1
-
-
-
-
-
1
-
-
-
-
4
-
-
1
-
-
1
-
-
1
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-
-
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2
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1
2
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1
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1
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1
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1
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-
699034
Dubey
A novel 9-bp insertion detecte ...
Homo sapiens
J. Biomed. Sci.
16
3-3
2009
-
-
-
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2
-
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-
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2
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2
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-
699173
Bratland
T cell responses to steroid cy ...
Homo sapiens
J. Clin. Endocrinol. Metab.
94
5117-5124
2009
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-
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3
-
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-
-
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1
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1
1
-
-
-
-
-
-
-
-
-
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-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
695941
Soardi
Heterozygosis for CYP21A2 muta ...
Homo sapiens
Arq. Bras. Endocrinol. Metabol.
52
1388-1392
2008
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
697429
Ono
In vitro enzyme assay of CYP21 ...
Homo sapiens
Clin. Pediatr. Endocrinol.
17
49-56
2008
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
698864
Tosha
Interaction between substrate ...
Bos taurus
J. Biol. Chem.
283
3708-3717
2008
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
699170
Riepe
Functional and structural cons ...
Homo sapiens
J. Clin. Endocrinol. Metab.
93
2891-2895
2008
-
-
1
-
2
-
-
5
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
2
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
688586
Trinh
Growth and pubertal characteri ...
Homo sapiens
J. Pediatr. Endocrinol. Metab.
20
883-891
2007
-
-
1
-
1
-
-
-
-
-
-
1
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
671760
Arase
Purification and characterizat ...
Bos taurus
Biochem. Biophys. Res. Commun.
344
400-405
2006
1
-
-
-
1
-
-
2
-
-
4
-
-
3
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
2
-
-
4
-
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
675491
Barbaro
Functional studies of two nove ...
Homo sapiens
J. Mol. Med.
84
521-528
2006
-
-
-
-
4
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
673435
Miguel
Analysis of the interaction be ...
Homo sapiens
Eur. J. Endocrinol.
153
949-961
2005
-
1
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
673436
Dolzan
Mutational spectrum of steroid ...
Homo sapiens
Eur. J. Endocrinol.
153
99-106
2005
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
673924
Supornsilchai
Phytoestrogen resveratrol supp ...
Rattus norvegicus
Horm. Res.
64
280-286
2005
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
675489
Krone
The residue E351 is essential ...
Homo sapiens
J. Mol. Med.
83
561-568
2005
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
658562
Kishimoto
Cytochrome P450 2D catalyze st ...
Rattus norvegicus
Endocrinology
145
699-705
2004
-
-
-
-
-
-
2
-
1
-
-
-
-
3
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
659570
Barbaro
Functional analysis of two rec ...
Homo sapiens
J. Clin. Endocrinol. Metab.
89
2402-2407
2004
-
-
-
-
2
-
-
6
-
-
-
-
-
2
-
-
-
-
-
1
6
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
6
-
-
-
-
-
-
-
-
-
1
6
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
658359
Lovelace
Steroid 21-hydroxylase express ...
Rattus norvegicus
Brain Res. Bull.
61
609-615
2003
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
659747
Li
Interrenal steroid 21-hydroxyl ...
Anguilla japonica
J. Mol. Endocrinol.
31
327-340
2003
-
-
1
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
659781
Felix-Lopez
Steroid 21-hydroxylase (P450c2 ...
Homo sapiens
J. Pediatr. Endocrinol. Metab.
16
1017-1024
2003
-
1
-
-
4
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
659808
Martineau
Molecular cloning and expressi ...
Cavia porcellus
J. Steroid Biochem. Mol. Biol.
86
123-132
2003
-
-
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285444
Blom
-
Characterisation of head kidne ...
Oncorhynchus mykiss
Fish Physiol. Biochem.
24
1-8
2001
-
-
-
-
-
-
1
2
-
-
-
1
-
1
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
1
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
285445
Belanger
Regulation of 21 hydroxylase a ...
Bos taurus
Endocr. Res.
21
329-41
1995
-
-
-
-
-
-
1
-
-
-
-
-
-
3
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285442
Haniu
Complete amino acid sequence o ...
Sus scrofa
Arch. Biochem. Biophys.
254
380-384
1987
-
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285449
Higashi
complete nucleotide sequence o ...
Homo sapiens
Proc. Natl. Acad. Sci. USA
83
2841-2845
1986
-
-
1
-
-
-
-
-
1
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285443
Narasimhulu
Adrenal microsomal hydroxylati ...
Bos taurus
Biochemistry
24
4287-4294
1985
-
-
-
-
-
-
-
-
1
-
1
-
-
1
-
-
1
-
-
1
2
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
1
-
1
2
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285446
Yuan
Steroid 21-hydroxylase (cytoch ...
Sus scrofa
Biochemistry
22
143-149
1983
-
-
-
-
-
-
-
2
-
-
1
-
-
5
-
-
1
-
-
1
-
-
2
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
2
-
-
1
-
-
-
-
1
-
1
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285448
Bumpus
Bovine adrenocortical microsom ...
Bos taurus
J. Biol. Chem.
257
12696-12704
1982
-
-
-
-
-
-
-
-
1
-
2
-
-
1
-
-
1
-
-
-
2
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
1
-
-
2
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285440
Hiwatashi
Purification and reconstitutio ...
Bos taurus
Biochim. Biophys. Acta
664
33-48
1981
6
-
-
-
-
-
5
-
1
-
1
-
-
2
-
1
1
-
-
1
3
1
4
1
-
-
-
-
1
-
-
1
-
-
-
6
-
-
1
-
-
-
-
5
-
-
1
-
1
-
-
-
1
1
-
1
3
1
4
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
285441
Greenfield
Activation and Inhibition of t ...
Bos taurus
Arch. Biochem. Biophys.
200
232-244
1980
8
-
-
-
-
-
2
-
1
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
285447
Kominami
Studies on the steroid hydroxy ...
Bos taurus
J. Biol. Chem.
255
3386-3394
1980
-
-
-
-
-
-
-
-
1
-
1
-
-
1
-
-
1
-
-
-
-
2
2
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
1
-
-
-
2
2
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
285439
Ryan
Hydroxylation of steroids at c ...
Bos taurus
J. Biol. Chem.
225
103-114
1957
-
-
-
-
-
-
12
-
1
-
-
1
-
1
-
-
-
-
-
1
-
-
7
-
-
-
-
-
1
1
-
2
-
-
-
-
-
-
2
-
-
-
-
12
-
-
1
-
-
1
-
-
-
-
-
1
-
-
7
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-