BRENDA - Enzyme Database show
show all sequences of 1.14.14.160

Characterization of CYP71Z18 indicates a role in maize zealexin biosynthesis

Mao, H.; Liu, J.; Ren, F.; Peters, R.J.; Wang, Q.; Phytochemistry 121, 4-10 (2016)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
CYP71Z18 is expressed in the C41 (DE3) OverExpress strain of Escherichia coli (Lucigen)
Zea mays
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(S)-beta-macrocarpene + 3 O2 + 3 [reduced NADPH-hemoprotein reductase]
Zea mays
the enzyme sequentially oxidizes (S)-beta-macrocarpene via alcohol and aldehyde intermediates to form zealexin A1, a maize phytoalexin that provides biochemical protection against fungal infection, overall reaction
zealexin A1 + 4 H2O + 3 [oxidized NADPH-hemoprotein reductase]
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Zea mays
B4FVP3
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(4S)-4-(5,5-dimethylcyclohex-1-en-1-yl)cyclohex-1-ene-1-carbaldehyde + O2 + [reduced NADPH-hemoprotein reductase]
the enzyme sequentially oxidizes(S)-beta-macrocarpene via alcohol and aldehyde intermediates to form zealexin A1
748880
Zea mays
zealexin A1 + H2O + [oxidized NADPH-hemoprotein reductase]
-
-
-
?
(S)-beta-macrocarpene + 3 O2 + 3 [reduced NADPH-hemoprotein reductase]
the enzyme sequentially oxidizes (S)-beta-macrocarpene via alcohol and aldehyde intermediates to form zealexin A1, a maize phytoalexin that provides biochemical protection against fungal infection, overall reaction
748880
Zea mays
zealexin A1 + 4 H2O + 3 [oxidized NADPH-hemoprotein reductase]
-
-
-
?
(S)-beta-macrocarpene + 3 O2 + 3 [reduced NADPH-hemoprotein reductase]
the enzyme sequentially oxidizes(S)-beta-macrocarpene via alcohol and aldehyde intermediates to form zealexin A1, overall reaction
748880
Zea mays
zealexin A1 + 4 H2O + 3 [oxidized NADPH-hemoprotein reductase]
-
-
-
?
(S)-beta-macrocarpene + O2 + [reduced NADPH-hemoprotein reductase]
the enzyme sequentially oxidizes(S)-beta-macrocarpene via alcohol and aldehyde intermediates to form zealexin A1
748880
Zea mays
[(4S)-4-(5,5-dimethylcyclohex-1-en-1-yl)-cyclohex-1-en-1-yl]methanol + H2O + [oxidized NADPH-hemoprotein reductase]
-
-
-
?
[(4S)-4-(5,5-dimethylcyclohex-1-en-1-yl)-cyclohex-1-en-1-yl] methanol + O2 + [reduced NADPH-hemoprotein reductase]
the enzyme sequentially oxidizes(S)-beta-macrocarpene via alcohol and aldehyde intermediates to form zealexin A1
748880
Zea mays
(4S)-4-(5,5-dimethylcyclohex-1-en-1-yl)cyclohex-1-ene-1-carbaldehyde + 2 H2O + [oxidized NADPH-hemoprotein reductase]
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P-450
a cytochrome P-450 (heme thiolate) enzyme
Zea mays
Cloned(Commentary) (protein specific)
Commentary
Organism
CYP71Z18 is expressed in the C41 (DE3) OverExpress strain of Escherichia coli (Lucigen)
Zea mays
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P-450
a cytochrome P-450 (heme thiolate) enzyme
Zea mays
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(S)-beta-macrocarpene + 3 O2 + 3 [reduced NADPH-hemoprotein reductase]
Zea mays
the enzyme sequentially oxidizes (S)-beta-macrocarpene via alcohol and aldehyde intermediates to form zealexin A1, a maize phytoalexin that provides biochemical protection against fungal infection, overall reaction
zealexin A1 + 4 H2O + 3 [oxidized NADPH-hemoprotein reductase]
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(4S)-4-(5,5-dimethylcyclohex-1-en-1-yl)cyclohex-1-ene-1-carbaldehyde + O2 + [reduced NADPH-hemoprotein reductase]
the enzyme sequentially oxidizes(S)-beta-macrocarpene via alcohol and aldehyde intermediates to form zealexin A1
748880
Zea mays
zealexin A1 + H2O + [oxidized NADPH-hemoprotein reductase]
-
-
-
?
(S)-beta-macrocarpene + 3 O2 + 3 [reduced NADPH-hemoprotein reductase]
the enzyme sequentially oxidizes (S)-beta-macrocarpene via alcohol and aldehyde intermediates to form zealexin A1, a maize phytoalexin that provides biochemical protection against fungal infection, overall reaction
748880
Zea mays
zealexin A1 + 4 H2O + 3 [oxidized NADPH-hemoprotein reductase]
-
-
-
?
(S)-beta-macrocarpene + 3 O2 + 3 [reduced NADPH-hemoprotein reductase]
the enzyme sequentially oxidizes(S)-beta-macrocarpene via alcohol and aldehyde intermediates to form zealexin A1, overall reaction
748880
Zea mays
zealexin A1 + 4 H2O + 3 [oxidized NADPH-hemoprotein reductase]
-
-
-
?
(S)-beta-macrocarpene + O2 + [reduced NADPH-hemoprotein reductase]
the enzyme sequentially oxidizes(S)-beta-macrocarpene via alcohol and aldehyde intermediates to form zealexin A1
748880
Zea mays
[(4S)-4-(5,5-dimethylcyclohex-1-en-1-yl)-cyclohex-1-en-1-yl]methanol + H2O + [oxidized NADPH-hemoprotein reductase]
-
-
-
?
[(4S)-4-(5,5-dimethylcyclohex-1-en-1-yl)-cyclohex-1-en-1-yl] methanol + O2 + [reduced NADPH-hemoprotein reductase]
the enzyme sequentially oxidizes(S)-beta-macrocarpene via alcohol and aldehyde intermediates to form zealexin A1
748880
Zea mays
(4S)-4-(5,5-dimethylcyclohex-1-en-1-yl)cyclohex-1-ene-1-carbaldehyde + 2 H2O + [oxidized NADPH-hemoprotein reductase]
-
-
-
?
Expression
Organism
Commentary
Expression
Zea mays
transcription is strongly induced by fungal infection
up
General Information
General Information
Commentary
Organism
metabolism
the enzyme sequentially oxidizes (S)-beta-macrocarpene via alcohol and aldehyde intermediates to form zealexin A1, a maize phytoalexin that provides biochemical protection against fungal infection
Zea mays
physiological function
the enzyme sequentially oxidizes (S)-beta-macrocarpene via alcohol and aldehyde intermediates to form zealexin A1, a maize phytoalexin that provides biochemical protection against fungal infection
Zea mays
General Information (protein specific)
General Information
Commentary
Organism
metabolism
the enzyme sequentially oxidizes (S)-beta-macrocarpene via alcohol and aldehyde intermediates to form zealexin A1, a maize phytoalexin that provides biochemical protection against fungal infection
Zea mays
physiological function
the enzyme sequentially oxidizes (S)-beta-macrocarpene via alcohol and aldehyde intermediates to form zealexin A1, a maize phytoalexin that provides biochemical protection against fungal infection
Zea mays
Expression (protein specific)
Organism
Commentary
Expression
Zea mays
transcription is strongly induced by fungal infection
up
Other publictions for EC 1.14.14.160
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
748880
Mao
Characterization of CYP71Z18 ...
Zea mays
Phytochemistry
121
4-10
2016
-
-
1
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
1
2
2
1
-
-