BRENDA - Enzyme Database show
show all sequences of 1.14.14.162

Identification of a cytochrome P450 cDNA encoding (2S)-flavanone 2-hydroxylase of licorice (Glycyrrhiza echinata L.; Fabaceae) which represents licodione synthase and flavone synthase II

Akashi, T.; Aoki, T.; Ayabe, S.; FEBS Lett. 431, 287-290 (1998)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
CYP Ge-5/CYP93B1, expression in Sf9 insect cells and in Saccharomyces cerevisiae BJ2168
Glycyrrhiza echinata
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
recombinant enzyme, expressed in Sf9 insect cells or yeast cells
Glycyrrhiza echinata
-
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Glycyrrhiza echinata
flavone biosynthesis
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Glycyrrhiza echinata
P93149
-
-
Reaction
Reaction
Commentary
Organism
a flavanone + [reduced NADPH-hemoprotein reductase] + O2 = a 2-hydroxyflavanone + [oxidized NADPH-hemoprotein reductase] + H2O
mechanism, stereoselectivity
Glycyrrhiza echinata
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(2S)-liquiritigenin + [reduced NADPH-hemoprotein reductase] + O2
hydroxylation of liquiritigenin at C-2 and formation of licodione as a result of non-enzymatic hemiacetal opening, selectivity towards (2S)-flavanone
655536
Glycyrrhiza echinata
licodione + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
(2S)-naringenin + [reduced NADPH-hemoprotein reductase] + O2
selectivity towards (2S)-flavanone
655536
Glycyrrhiza echinata
2-hydroxynaringenin + [oxidized NADPH-hemoprotein reductase] + H2O
the product is further converted into apigenin when treated with HCl
-
-
?
eriodictyol + [reduced NADPH-hemoprotein reductase] + O2
-
655536
Glycyrrhiza echinata
luteolin + [oxidized NADPH-hemoprotein reductase] + H2O
product is formed after acid treatment
-
-
?
additional information
flavone biosynthesis
655536
Glycyrrhiza echinata
?
-
-
-
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Glycyrrhiza echinata
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Glycyrrhiza echinata
Cloned(Commentary) (protein specific)
Commentary
Organism
CYP Ge-5/CYP93B1, expression in Sf9 insect cells and in Saccharomyces cerevisiae BJ2168
Glycyrrhiza echinata
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Glycyrrhiza echinata
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
recombinant enzyme, expressed in Sf9 insect cells or yeast cells
Glycyrrhiza echinata
-
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Glycyrrhiza echinata
flavone biosynthesis
?
-
-
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(2S)-liquiritigenin + [reduced NADPH-hemoprotein reductase] + O2
hydroxylation of liquiritigenin at C-2 and formation of licodione as a result of non-enzymatic hemiacetal opening, selectivity towards (2S)-flavanone
655536
Glycyrrhiza echinata
licodione + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
(2S)-naringenin + [reduced NADPH-hemoprotein reductase] + O2
selectivity towards (2S)-flavanone
655536
Glycyrrhiza echinata
2-hydroxynaringenin + [oxidized NADPH-hemoprotein reductase] + H2O
the product is further converted into apigenin when treated with HCl
-
-
?
eriodictyol + [reduced NADPH-hemoprotein reductase] + O2
-
655536
Glycyrrhiza echinata
luteolin + [oxidized NADPH-hemoprotein reductase] + H2O
product is formed after acid treatment
-
-
?
additional information
flavone biosynthesis
655536
Glycyrrhiza echinata
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Glycyrrhiza echinata
Other publictions for EC 1.14.14.162
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
748594
Vanegas
Indirect and direct routes to ...
Oryza sativa Japonica Group
Microb. Cell Fact.
17
107
2018
-
1
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-
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-
-
-
-
-
2
-
4
-
-
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2
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1
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1
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1
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2
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2
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-
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748966
Du
CYP93G2 is a flavanone 2-hydr ...
Oryza sativa
Plant Physiol.
154
324-333
2010
-
-
1
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-
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2
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5
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2
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1
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1
1
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2
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2
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2
2
-
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-
655536
Akashi
Identification of a cytochrome ...
Glycyrrhiza echinata
FEBS Lett.
431
287-290
1998
-
-
1
-
-
-
-
-
1
-
-
1
-
3
-
-
-
1
-
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-
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4
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1
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-
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-
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1
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1
1
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1
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1
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4
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1
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657025
Otani
Licodione synthase, a cytochro ...
Glycyrrhiza echinata
Plant Physiol.
105
1427-1432
1994
1
-
-
-
-
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3
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1
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1
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1
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1
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1
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4
-
1
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-
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1
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1
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1
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-
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3
-
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1
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1
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1
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4
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1
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