BRENDA - Enzyme Database show
show all sequences of 1.14.14.162

Indirect and direct routes to C-glycosylated flavones in Saccharomyces cerevisiae

Vanegas, K.G.; Larsen, A.B.; Eichenberger, M.; Fischer, D.; Mortensen, U.H.; Naesby, M.; Microb. Cell Fact. 17, 107 (2018)

Data extracted from this reference:

Application
Application
Commentary
Organism
synthesis
two-step indirect glycosylation using combinations of flavanone 2-hydroxylase and C-glycosyltransferases, to convert 2-hydroxyflavanone intermediates into the 6C-glucoside flavones isovitexin and isoorientin, and the 8C-glucoside flavones vitexin and orientin. The ratio between 6C and 8C glycosylation depended on the C-glycosyltransferase used. The indirect route resuls in mixtures, similar to what has been reported for in vitro experiments
Oryza sativa Japonica Group
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
eriodictyol+ [reduced NADPH-hemoprotein reductase] + O2
Oryza sativa Japonica Group
-
2-hydroxyeriodictyol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
naringenin + [reduced NADPH-hemoprotein reductase] + O2
Oryza sativa Japonica Group
-
2-hydroxynaringenin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Oryza sativa Japonica Group
Q5VRI5
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
eriodictyol+ [reduced NADPH-hemoprotein reductase] + O2
-
748594
Oryza sativa Japonica Group
2-hydroxyeriodictyol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
naringenin + [reduced NADPH-hemoprotein reductase] + O2
-
748594
Oryza sativa Japonica Group
2-hydroxynaringenin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P-450
a cytochrome P-450 (heme thiolate) enzyme
Oryza sativa Japonica Group
Application (protein specific)
Application
Commentary
Organism
synthesis
two-step indirect glycosylation using combinations of flavanone 2-hydroxylase and C-glycosyltransferases, to convert 2-hydroxyflavanone intermediates into the 6C-glucoside flavones isovitexin and isoorientin, and the 8C-glucoside flavones vitexin and orientin. The ratio between 6C and 8C glycosylation depended on the C-glycosyltransferase used. The indirect route resuls in mixtures, similar to what has been reported for in vitro experiments
Oryza sativa Japonica Group
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P-450
a cytochrome P-450 (heme thiolate) enzyme
Oryza sativa Japonica Group
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
eriodictyol+ [reduced NADPH-hemoprotein reductase] + O2
Oryza sativa Japonica Group
-
2-hydroxyeriodictyol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
naringenin + [reduced NADPH-hemoprotein reductase] + O2
Oryza sativa Japonica Group
-
2-hydroxynaringenin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
eriodictyol+ [reduced NADPH-hemoprotein reductase] + O2
-
748594
Oryza sativa Japonica Group
2-hydroxyeriodictyol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
naringenin + [reduced NADPH-hemoprotein reductase] + O2
-
748594
Oryza sativa Japonica Group
2-hydroxynaringenin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Other publictions for EC 1.14.14.162
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
748594
Vanegas
Indirect and direct routes to ...
Oryza sativa Japonica Group
Microb. Cell Fact.
17
107
2018
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2
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748966
Du
CYP93G2 is a flavanone 2-hydr ...
Oryza sativa
Plant Physiol.
154
324-333
2010
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1
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2
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5
-
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1
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2
2
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655536
Akashi
Identification of a cytochrome ...
Glycyrrhiza echinata
FEBS Lett.
431
287-290
1998
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1
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1
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3
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1
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657025
Otani
Licodione synthase, a cytochro ...
Glycyrrhiza echinata
Plant Physiol.
105
1427-1432
1994
1
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-
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3
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1
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1
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1
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1
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1
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4
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1
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1
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1
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1
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3
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1
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