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Literature summary for 1.14.14.163 extracted from

  • Dang, T.; Chen, X.; Facchini, P.
    Acetylation serves as a protective group in noscapine biosynthesis in opium poppy (2015), Nat. Chem. Biol., 11, 104-106 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Saccharomyces cerevisiae. Owing to difficulties with heterologous expression in yeast, CYP82X2 is synthesized with the first 60 N-terminal amino acids replaced with the first 43 N-terminal amino acids from the lettuce cytochrome P450 germacrene A oxidase Papaver somniferum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0178
-
(S)-1-hydroxy-N-methylcanadine pH 7.5, 30°C Papaver somniferum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-1-hydroxy-N-methylcanadine + [reduced NADPH-hemoprotein reductase] + O2 Papaver somniferum the enzyme participates in the biosynthesis of the isoquinoline alkaloid noscapine (13S,14R)-1,13-dihydroxy-N-methylcanadine + [oxidized NADPH-hemoprotein reductase] + H2O
-
?

Organism

Organism UniProt Comment Textmining
Papaver somniferum I3PLR0
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Papaver somniferum

Source Tissue

Source Tissue Comment Organism Textmining
stem
-
Papaver somniferum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-1-hydroxy-N-methylcanadine + [reduced NADPH-hemoprotein reductase] + O2 the enzyme participates in the biosynthesis of the isoquinoline alkaloid noscapine Papaver somniferum (13S,14R)-1,13-dihydroxy-N-methylcanadine + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
(S)-1-hydroxy-N-methylcanadine + [reduced NADPH-hemoprotein reductase] + O2 the enzyme shows strict substrate specificity Papaver somniferum (13S,14R)-1,13-dihydroxy-N-methylcanadine + [oxidized NADPH-hemoprotein reductase] + H2O
-
?

Synonyms

Synonyms Comment Organism
CYP82X2
-
Papaver somniferum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Papaver somniferum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Papaver somniferum

General Information

General Information Comment Organism
malfunction CYP719A21, CYP82X1 and CXE1 showed reduced transcript levels in CYP82X2-silenced plants Papaver somniferum
metabolism the enzyme participates in the biosynthesis of the isoquinoline alkaloid noscapine Papaver somniferum