BRENDA - Enzyme Database show
show all sequences of 1.14.14.164

In planta biocatalysis screen of P450s identifies 8-methoxypsoralen as a substrate for the CYP82C subfamily, yielding original chemical structures

Kruse, T.; Ho, K.; Yoo, H.D.; Johnson, T.; Hippely, M.; Park, J.H.; Flavell, R.; Bobzin, S.; Chem. Biol. 15, 149-156 (2008)

Data extracted from this reference:

Application
Application
Commentary
Organism
synthesis
the therapeutic compound 8-methoxypsoralen is hydroxylated by plants overexpressing CYP82C2 or CYP82C4, forming 5-hydroxy-8-methoxypsoralen
Arabidopsis thaliana
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arabidopsis thaliana
Q9SZ46
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
8-methoxypsoralen + [reduced NADPH-hemoprotein reductase] + O2
-
747494
Arabidopsis thaliana
5-hydroxy-8-methoxypsoralen + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P-450
a cytochrome P-450 (heme thiolate) enzyme
Arabidopsis thaliana
Application (protein specific)
Application
Commentary
Organism
synthesis
the therapeutic compound 8-methoxypsoralen is hydroxylated by plants overexpressing CYP82C2 or CYP82C4, forming 5-hydroxy-8-methoxypsoralen
Arabidopsis thaliana
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P-450
a cytochrome P-450 (heme thiolate) enzyme
Arabidopsis thaliana
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
8-methoxypsoralen + [reduced NADPH-hemoprotein reductase] + O2
-
747494
Arabidopsis thaliana
5-hydroxy-8-methoxypsoralen + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Other publictions for EC 1.14.14.164
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
748735
Rajniak
Biosynthesis of redox-active ...
Arabidopsis thaliana
Nat. Chem. Biol.
14
442-450
2018
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1
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1
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1
-
-
1
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1
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3
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1
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1
1
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1
-
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1
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1
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3
-
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1
1
1
1
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748476
Murgia
Arabidopsis CYP82C4 expressio ...
Arabidopsis thaliana
J. Plant Physiol.
168
894-902
2011
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3
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2
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1
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1
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2
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1
1
1
1
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747494
Kruse
In planta biocatalysis screen ...
Arabidopsis thaliana
Chem. Biol.
15
149-156
2008
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