BRENDA - Enzyme Database show
show all sequences of 1.14.14.164

Biosynthesis of redox-active metabolites in response to iron deficiency in plants

Rajniak, J.; Giehl, R.; Chang, E.; Murgia, I.; Von Wiren, N.; Sattely, E.; Nat. Chem. Biol. 14, 442-450 (2018)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
heterologous-pathway reconstitution in Nicotiana benthamiana
Arabidopsis thaliana
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
fraxetin + [reduced NADPH-hemoprotein reductase] + O2
Arabidopsis thaliana
the enzyme is involved in biosynthesis of iron(III)-chelating coumarins in higher plants
sideretin (reduced form) + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arabidopsis thaliana
Q9SZ46
-
-
Purification (Commentary)
Commentary
Organism
-
Arabidopsis thaliana
Source Tissue
Source Tissue
Commentary
Organism
Textmining
root
expressed most strongly in the root epidermal layer under iron-deficient conditions
Arabidopsis thaliana
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
8-methoxypsoralen + [reduced NADPH-hemoprotein reductase] + O2
-
748735
Arabidopsis thaliana
5-hydroxy-8-methoxypsoralen + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
fraxetin + [reduced NADPH-hemoprotein reductase] + O2
-
748735
Arabidopsis thaliana
sideretin (reduced form) + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
fraxetin + [reduced NADPH-hemoprotein reductase] + O2
the enzyme is involved in biosynthesis of iron(III)-chelating coumarins in higher plants
748735
Arabidopsis thaliana
sideretin (reduced form) + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P-450
a cytochrome P-450 (heme thiolate) enzyme
Arabidopsis thaliana
Cloned(Commentary) (protein specific)
Commentary
Organism
heterologous-pathway reconstitution in Nicotiana benthamiana
Arabidopsis thaliana
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P-450
a cytochrome P-450 (heme thiolate) enzyme
Arabidopsis thaliana
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
fraxetin + [reduced NADPH-hemoprotein reductase] + O2
Arabidopsis thaliana
the enzyme is involved in biosynthesis of iron(III)-chelating coumarins in higher plants
sideretin (reduced form) + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Arabidopsis thaliana
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
root
expressed most strongly in the root epidermal layer under iron-deficient conditions
Arabidopsis thaliana
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
8-methoxypsoralen + [reduced NADPH-hemoprotein reductase] + O2
-
748735
Arabidopsis thaliana
5-hydroxy-8-methoxypsoralen + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
fraxetin + [reduced NADPH-hemoprotein reductase] + O2
-
748735
Arabidopsis thaliana
sideretin (reduced form) + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
fraxetin + [reduced NADPH-hemoprotein reductase] + O2
the enzyme is involved in biosynthesis of iron(III)-chelating coumarins in higher plants
748735
Arabidopsis thaliana
sideretin (reduced form) + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Expression
Organism
Commentary
Expression
Arabidopsis thaliana
expression is upregulated by iron deficiency. The iron-deficiency-dependent upregulation is mainly confined to roots
up
General Information
General Information
Commentary
Organism
metabolism
the enzyme is involved in biosynthesis of iron(III)-chelating coumarins in higher plants. Under iron-deficiency conditions, a branch of the phenylpropanoid pathway consisting of feruloyl-CoA 6'-hydroxylase 1, scopoletin 8-hydroxylase, and CYP82C4 is strongly induced, thus leading to the synthesis of scopoletin and its conversion into redox-active fraxetin and sideretin. In the rhizosphere, the redox-active coumarins act in concert with other components of the strategy I iron acquisition machinery by solubilizing and reducing iron from sparingly available sources
Arabidopsis thaliana
General Information (protein specific)
General Information
Commentary
Organism
metabolism
the enzyme is involved in biosynthesis of iron(III)-chelating coumarins in higher plants. Under iron-deficiency conditions, a branch of the phenylpropanoid pathway consisting of feruloyl-CoA 6'-hydroxylase 1, scopoletin 8-hydroxylase, and CYP82C4 is strongly induced, thus leading to the synthesis of scopoletin and its conversion into redox-active fraxetin and sideretin. In the rhizosphere, the redox-active coumarins act in concert with other components of the strategy I iron acquisition machinery by solubilizing and reducing iron from sparingly available sources
Arabidopsis thaliana
Expression (protein specific)
Organism
Commentary
Expression
Arabidopsis thaliana
expression is upregulated by iron deficiency. The iron-deficiency-dependent upregulation is mainly confined to roots
up
Other publictions for EC 1.14.14.164
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
748735
Rajniak
Biosynthesis of redox-active ...
Arabidopsis thaliana
Nat. Chem. Biol.
14
442-450
2018
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1
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1
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1
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3
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1
1
1
1
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748476
Murgia
Arabidopsis CYP82C4 expressio ...
Arabidopsis thaliana
J. Plant Physiol.
168
894-902
2011
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3
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2
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1
1
1
1
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747494
Kruse
In planta biocatalysis screen ...
Arabidopsis thaliana
Chem. Biol.
15
149-156
2008
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1
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3
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