BRENDA - Enzyme Database show
show all sequences of 1.14.14.165

A new cyanogenic metabolite in Arabidopsis required for inducible pathogen defence

Rajniak, J.; Barco, B.; Clay, N.K.; Sattely, E.S.; Nature 525, 376-379 (2015)

Data extracted from this reference:

Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
indole-3-carbonyl nitrile + [reduced NADPH-hemoprotein reductase] + O2
Arabidopsis thaliana
the enzyme is involved in biosynthesis of small cyanogenic compounds that take part in pathogen defense
4-hydroxyindole-3-carbonyl nitrile + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arabidopsis thaliana
O49394
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
indole-3-carbonyl nitrile + [reduced NADPH-hemoprotein reductase] + O2
-
748762
Arabidopsis thaliana
4-hydroxyindole-3-carbonyl nitrile + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
indole-3-carbonyl nitrile + [reduced NADPH-hemoprotein reductase] + O2
the enzyme is involved in biosynthesis of small cyanogenic compounds that take part in pathogen defense
748762
Arabidopsis thaliana
4-hydroxyindole-3-carbonyl nitrile + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P-450
a cytochrome P-450 (heme thiolate) enzyme
Arabidopsis thaliana
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P-450
a cytochrome P-450 (heme thiolate) enzyme
Arabidopsis thaliana
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
indole-3-carbonyl nitrile + [reduced NADPH-hemoprotein reductase] + O2
Arabidopsis thaliana
the enzyme is involved in biosynthesis of small cyanogenic compounds that take part in pathogen defense
4-hydroxyindole-3-carbonyl nitrile + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
indole-3-carbonyl nitrile + [reduced NADPH-hemoprotein reductase] + O2
-
748762
Arabidopsis thaliana
4-hydroxyindole-3-carbonyl nitrile + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
indole-3-carbonyl nitrile + [reduced NADPH-hemoprotein reductase] + O2
the enzyme is involved in biosynthesis of small cyanogenic compounds that take part in pathogen defense
748762
Arabidopsis thaliana
4-hydroxyindole-3-carbonyl nitrile + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Expression
Organism
Commentary
Expression
Arabidopsis thaliana
highly expressed under a variety of pathogen treatment conditions. Expression is strongly upregulated 24 h after inoculation with the bacterial pathogen Pseudomonas syringae pv. tomato DC3000
up
General Information
General Information
Commentary
Organism
metabolism
the enzyme is involved in biosynthesis of small cyanogenic compounds that take part in pathogen defense
Arabidopsis thaliana
General Information (protein specific)
General Information
Commentary
Organism
metabolism
the enzyme is involved in biosynthesis of small cyanogenic compounds that take part in pathogen defense
Arabidopsis thaliana
Expression (protein specific)
Organism
Commentary
Expression
Arabidopsis thaliana
highly expressed under a variety of pathogen treatment conditions. Expression is strongly upregulated 24 h after inoculation with the bacterial pathogen Pseudomonas syringae pv. tomato DC3000
up
Other publictions for EC 1.14.14.165
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
748762
Rajniak
A new cyanogenic metabolite i ...
Arabidopsis thaliana
Nature
525
376-379
2015
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2
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2
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1
1
1
1
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747485
Liu
The Arabidopsis P450 protein ...
Arabidopsis thaliana
Cell Res.
20
539-552
2010
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1
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4
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4
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1
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1
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4
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2
2
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747494
Kruse
In planta biocatalysis screen ...
Arabidopsis thaliana
Chem. Biol.
15
149-156
2008
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1
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3
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1
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