Application | Comment | Organism |
---|---|---|
medicine | cholesterol lowering effect of green tea may be attributed to the enzyme inhibitory activities of its gallocatechins | Rattus norvegicus |
Cloned (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(-)-epicatechin-3-O-gallate | 50% inhibition at 0.0013 mM | Rattus norvegicus | |
(-)-epigallocatechin-3-O-gallate | 50% inhibition at 0.00069 mM, noncompetitive | Rattus norvegicus | |
(-)-gallocatechin-3-O-gallate | 50% inhibition at 0.00067 mM | Rattus norvegicus | |
epigallocatechin-3-O-gallate | - |
Rattus norvegicus | |
additional information | cholesterol lowering effect of green tea gallocatechins may be attributed to their potent squalene oxidase inhibition; not inhibitory: flavan-3-ols with galloyl group at C-3 | Rattus norvegicus | |
theasinensin A | 50% inhibition at 0.00013 mM | Rattus norvegicus | |
trisnorsqualene alcohol | 0.004 mM, 50% inhibition | Sus scrofa | |
trisnorsqualene cyclopropylamine | 0.002 mM, 50% inhibition | Sus scrofa | |
trisnorsqualene difluoromethylidene | 0.0054 mM, 50% inhibition | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0036 | - |
squalene | - |
Rattus norvegicus | |
0.0038 | - |
squalene | 37°C, pH 7.4 | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
squalene + [reduced NADPH-hemoprotein reductase] + O2 = (3S)-2,3-epoxy-2,3-dihydrosqualene + [oxidized NADPH-hemoprotein reductase] + H2O | proposed mechanism | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
squalene + electron donor + O2 | - |
Rattus norvegicus | (S)-squalene-2,3-epoxide + oxidized electron donor + H2O | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0683 | - |
squalene | N-terminal trucated recombinant enzyme | Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00074 | - |
epigallocatechin-3-O-gallate | non-competitive, non-time dependent inhibition | Rattus norvegicus | |
0.00074 | - |
(-)-epigallocatechin-3-O-gallate | 37°C, pH 7.4 | Rattus norvegicus |