Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Triton X-100 | activates at 0.1% | Methylococcus capsulatus |
Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, functional expression in Escherichia coli strain BL21(DE3) | Methylococcus capsulatus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
squalene + reduced cytochrome P450 + O2 | Methylococcus capsulatus | first step in the cyclic chloesterol biosynthesis | (S)-squalene-2,3-epoxide + cytochrome P450 + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methylococcus capsulatus | Q603D5 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
squalene + reduced cytochrome P450 + H+ + O2 | absolutely stereo- and regiospecific reaction, co-reaction with NADPH-cytochrome P450 reductase | Methylococcus capsulatus | (3S)-squalene-2,3-epoxide + cytochrome P450 + H2O | - |
? | |
squalene + reduced cytochrome P450 + O2 | first step in the cyclic chloesterol biosynthesis | Methylococcus capsulatus | (S)-squalene-2,3-epoxide + cytochrome P450 + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
squalene epoxidase | - |
Methylococcus capsulatus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Methylococcus capsulatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Methylococcus capsulatus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Methylococcus capsulatus | |
reduced cytochrome P450 | - |
Methylococcus capsulatus |