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Literature summary for 1.14.14.18 extracted from
- Structure of the Escherichia coli O157:H7 heme oxygenase ChuS in complex with heme and enzymatic inactivation by mutation of the heme coordinating residue His-193 (2006), J. Biol. Chem., 281, 36776-36782.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Escherichia coli |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| sitting drop vapour diffusion method using 12-15% (w/v) polyethylene glycol 3350, 0.15-0.20 M magnesium formate, and 10-20 mM NAD | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| H193N | inactive enzyme | Escherichia coli |
| H73A | no spectrum compared with native ChuS | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography and Resource Q column chromatography | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| heme + NADH + O2 | - |
Escherichia coli | biliverdin + Fe2+ + CO + NAD+ + H2O | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ChuS | - |
Escherichia coli |
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Release 2023.1 (January 2023)
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