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Literature summary for 1.14.14.18 extracted from
- Molecular cloning, characterization, and expression of an alfalfa (Medicago sativa L.) heme oxygenase-1 gene, MsHO1, which is pro-oxidants-regulated (2011), Plant Physiol. Biochem., 49, 792-799.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of GFP-tagged HO1 targeted to chloroplasts. Expression of mature MsHO1 in Escherichia coli | Medicago sativa |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00236 | - |
heme | pH 7.2, 37°C, recombinant enzyme | Medicago sativa |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | - |
Medicago sativa | 9507 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Medicago sativa | D9IWR7 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Medicago sativa | - |
| additional information | expressions of MsHO1 are higher in alfalfa stems and leaves than those in germinating seeds and roots, expression patterns, overview | Medicago sativa | - |
| root | - |
Medicago sativa | - |
| seed | - |
Medicago sativa | - |
| stem | - |
Medicago sativa | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| heme + 3 AH2 + 3 O2 | - |
Medicago sativa | biliverdin + Fe2+ + CO + 3 A + 3 H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| heme oxygenase-1 | - |
Medicago sativa |
| HO-1 | - |
Medicago sativa |
| MsHO1 | - |
Medicago sativa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Medicago sativa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
- |
Medicago sativa |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Medicago sativa | MsHO1 gene expression and protein level are induced significantly by some pro-oxidant compounds, including hemin and nitric oxide donor sodium nitroprusside | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | MsHO1 may play an important role in oxidative responses | Medicago sativa |
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