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Literature summary for 1.14.14.19 extracted from

  • Pechurskaya, T.A.; Lukashevich, O.P.; Gilep, A.A.; Usanov, S.A.
    Engineering, expression, and purification of "soluble" human cytochrome P45017alpha and its functional characterization (2008), Biochemistry (Moscow), 73, 806-811.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain JM109 Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information construction of a soluble enzyme form by N-terminal truncations, CYP17mod containing hydrophilic FG-loop, a mutant with deleted hydrophobic N-terminal sequence DELTA23 and with a substituted cluster of hydrophobic amino acid residues in the region of the FG-loop, is mostly localized in the cytosolic fraction, replacement of hydrophobic amino acid residues in the FG-loop region does not change the metabolic profile during hydroxylation of steroids that is characteristic for wild type CYP17, overview Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane bound Homo sapiens 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+
-
Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens CYP17 is a key enzyme in steroid hormone biosynthesis ?
-
?
pregnenolone + AH2 + O2 Homo sapiens
-
17alpha-hydroxypregnenolone + A + H2O
-
?
progesterone + AH2 + O2 Homo sapiens
-
17alpha-hydroxyprogesterone + A + H2O
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain JM109 by anion exchange and nickel affinity chromatography, hydroxyapatite chromatography, and gel filtration Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
17alpha-hydroxylase and 17,20-lyase activity of recombinant wild-type and mutant enzymes Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information CYP17 is a key enzyme in steroid hormone biosynthesis Homo sapiens ?
-
?
additional information the bifunctional enzyme also exhibits 17,20-lyase activity converting 17alpha-hydroxypregnenolone to dehydroepiandrosterone-S, EC 4.1.2.30, overview Homo sapiens ?
-
?
pregnenolone + AH2 + O2
-
Homo sapiens 17alpha-hydroxypregnenolone + A + H2O
-
?
progesterone + AH2 + O2
-
Homo sapiens 17alpha-hydroxyprogesterone + A + H2O
-
?

Subunits

Subunits Comment Organism
More modeling of the tertiary structure of CYP17 Homo sapiens

Synonyms

Synonyms Comment Organism
CYP17
-
Homo sapiens
cytochrome P45017alpha
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
cytochrome P450
-
Homo sapiens
heme computer model of the tertiary structure of the hemeprotein Homo sapiens