Cloned (Comment) | Organism |
---|---|
expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain JM109 | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a soluble enzyme form by N-terminal truncations, CYP17mod containing hydrophilic FG-loop, a mutant with deleted hydrophobic N-terminal sequence DELTA23 and with a substituted cluster of hydrophobic amino acid residues in the region of the FG-loop, is mostly localized in the cytosolic fraction, replacement of hydrophobic amino acid residues in the FG-loop region does not change the metabolic profile during hydroxylation of steroids that is characteristic for wild type CYP17, overview | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | bound | Homo sapiens | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | - |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | CYP17 is a key enzyme in steroid hormone biosynthesis | ? | - |
? | |
pregnenolone + AH2 + O2 | Homo sapiens | - |
17alpha-hydroxypregnenolone + A + H2O | - |
? | |
progesterone + AH2 + O2 | Homo sapiens | - |
17alpha-hydroxyprogesterone + A + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain JM109 by anion exchange and nickel affinity chromatography, hydroxyapatite chromatography, and gel filtration | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
17alpha-hydroxylase and 17,20-lyase activity of recombinant wild-type and mutant enzymes | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | CYP17 is a key enzyme in steroid hormone biosynthesis | Homo sapiens | ? | - |
? | |
additional information | the bifunctional enzyme also exhibits 17,20-lyase activity converting 17alpha-hydroxypregnenolone to dehydroepiandrosterone-S, EC 4.1.2.30, overview | Homo sapiens | ? | - |
? | |
pregnenolone + AH2 + O2 | - |
Homo sapiens | 17alpha-hydroxypregnenolone + A + H2O | - |
? | |
progesterone + AH2 + O2 | - |
Homo sapiens | 17alpha-hydroxyprogesterone + A + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | modeling of the tertiary structure of CYP17 | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
CYP17 | - |
Homo sapiens |
cytochrome P45017alpha | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome P450 | - |
Homo sapiens | |
heme | computer model of the tertiary structure of the hemeprotein | Homo sapiens |