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Literature summary for 1.14.14.19 extracted from
- A role for cytochrome P450 17alpha-hydroxylase/c17-20 lyase during shift in steroidogenesis occurring in ovarian follicles prior to oocyte maturation (2009), J. Steroid Biochem. Mol. Biol., 115, 77-85.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene p450c17, cDNA from ovary, DNA and amino acid sequence determination and analysis, phylogenetic tree, transient expression in COS-7 cells, overexpression in Escherichia coli | Clarias gariepinus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clarias gariepinus | C7B608 | air-breathing catfish, gene p450c17 | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Clarias gariepinus | - |
| gill | - |
Clarias gariepinus | - |
| gonad | - |
Clarias gariepinus | - |
| heart | - |
Clarias gariepinus | - |
| interstitial cell | - |
Clarias gariepinus | - |
| intestine | - |
Clarias gariepinus | - |
| kidney | - |
Clarias gariepinus | - |
| additional information | tissue- and developmental stage-dependent expression patterns, overview. p450c17 expression and ratio of lyase to hydroxylase is high in preparatory and pre-spawning phases of ovary and low in spawning phase. Expression of p450c17 correlats well with testicular recrudescence with maximum expression in preparatory and spawning phases. Neither protein expression nor lyase/hydroxylase activity change significantly during hCG-induced oocyte maturation, while in vitro and in vivo though mRNA levels increase | Clarias gariepinus | - |
| muscle | - |
Clarias gariepinus | - |
| oocyte | - |
Clarias gariepinus | - |
| ovarian follicle | - |
Clarias gariepinus | - |
| ovary | - |
Clarias gariepinus | - |
| spermatocyte | testicular | Clarias gariepinus | - |
| spleen | - |
Clarias gariepinus | - |
| testis | - |
Clarias gariepinus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | P450c17 is an enzyme type that lacks lyase activity | Clarias gariepinus | ? | - |
? |  |
| progesterone + 2 NADPH + 2 H+ + 2 O2 | - |
Clarias gariepinus | androstenedione + acetate + 2 NADP+ + 2 H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| cytochrome P450 17alpha-hydroxylase/c17-20 lyase | - |
Clarias gariepinus |
| P450c17 | - |
Clarias gariepinus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Clarias gariepinus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Clarias gariepinus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Clarias gariepinus | |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Clarias gariepinus | p450c17 expression and ratio of lyase to hydroxylase is high in preparatory and pre-spawning phases of ovary and low in spawning phase. Expression of p450c17 correlates well with testicular recrudescence with maximum expression in preparatory and spawning phases. Neither protein expression nor lyase/hydroxylase activity change significantly during hCG-induced oocyte maturation, while in vitro and in vivo though mRNA levels increase | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | cytochromeP450 17alpha-hydroxylase/c17-20 lyase is one of the key enzymes involved in the steroidogenic shift that occurs prior to oocyte maturation in teleosts | Clarias gariepinus |
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