Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21-Gold (DE3) cells | Danio rerio |
Crystallization (Comment) | Organism |
---|---|
isoform P450 17A1 in complex with either abiraterone or progesterone, sitting drop vapor diffusion method, using 100 mM sodium HEPES buffer (pH 7.5), 50 mM MgCl2, and 20-30% (w/v) polyethylene glycol monomethyl ether 3550 | Danio rerio |
isoform P450 17A2 in complex with either abiraterone or progesterone, sitting drop vapor diffusion method, using 100 mM sodium HEPES buffer (pH 7.5), 50 mM MgCl2, and 20-30% (w/v) polyethylene glycol monomethyl ether 3550 | Danio rerio |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
abiraterone | - |
Danio rerio | |
orteronel | - |
Danio rerio |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0017 | - |
progesterone | isoform P450 17A1, at pH 7.4 and 37°C | Danio rerio | |
0.002 | - |
pregnenolone | isoform P450 17A1, at pH 7.4 and 37°C | Danio rerio | |
0.0093 | - |
pregnenolone | isoform P450 17A2, at pH 7.4 and 37°C | Danio rerio | |
0.03 | - |
progesterone | isoform P450 17A2, at pH 7.4 and 37°C | Danio rerio |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
51500 | - |
x * 51500, isoform P450 17A1, calculated from amino acid sequence | Danio rerio |
54000 | - |
x * 54000, isoform P450 17A2, calculated from amino acid sequence | Danio rerio |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Danio rerio | A7U483 | - |
- |
Danio rerio | B3DH80 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography, DEAE column chromatography, and SP-Sepharose column chromatography | Danio rerio |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | isoform P450 17A1 also exhibits 17alpha,20-lyase activity | Danio rerio | ? | - |
? | |
pregnenolone + [reduced NADPH-hemoprotein reductase] + O2 | - |
Danio rerio | 17alpha-hydroxypregnenolone + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
progesterone + [reduced NADPH-hemoprotein reductase] + O2 | - |
Danio rerio | 17alpha-hydroxyprogesterone + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
progesterone + [reduced NADPH-hemoprotein reductase] + O2 | isoform P450 17A1 has 3fold higher catalytic efficiency in the 17alpha-hydroxylation of progesterone than pregnenolone | Danio rerio | 17alpha-hydroxyprogesterone + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 51500, isoform P450 17A1, calculated from amino acid sequence | Danio rerio |
? | x * 54000, isoform P450 17A2, calculated from amino acid sequence | Danio rerio |
Synonyms | Comment | Organism |
---|---|---|
cytochrome P450 17A1 | - |
Danio rerio |
cytochrome P450 17A2 | - |
Danio rerio |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
pregnenolone | isoform P450 17A1, at pH 7.4 and 37°C | Danio rerio | |
0.1 | - |
progesterone | isoform P450 17A1, at pH 7.4 and 37°C | Danio rerio | |
0.85 | - |
pregnenolone | isoform P450 17A2, at pH 7.4 and 37°C | Danio rerio | |
1.6 | - |
progesterone | isoform P450 17A2, at pH 7.4 and 37°C | Danio rerio |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00000035 | - |
isoform P450 17A1, with pregnenolone as substrate, at pH 7.4b and 37°C | Danio rerio | orteronel | |
0.0000011 | - |
isoform P450 17A2, with progesterone as substrate, at pH 7.4b and 37°C | Danio rerio | orteronel | |
0.000015 | - |
isoform P450 17A1, with progesterone as substrate, at pH 7.4b and 37°C | Danio rerio | orteronel | |
0.00079 | - |
isoform P450 17A2, with pregnenolone as substrate, at pH 7.4b and 37°C | Danio rerio | orteronel |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
16.7 | - |
pregnenolone | isoform P450 17A1, at pH 7.4 and 37°C | Danio rerio | |
53.3 | - |
progesterone | isoform P450 17A2, at pH 7.4 and 37°C | Danio rerio | |
56.7 | - |
progesterone | isoform P450 17A1, at pH 7.4 and 37°C | Danio rerio | |
91.7 | - |
pregnenolone | isoform P450 17A2, at pH 7.4 and 37°C | Danio rerio |