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Literature summary for 1.14.14.20 extracted from

  • Szoekoel, J.; Rucka, L.; Simcikova, M.; Halada, P.; Nesvera, J.; Patek, M.
    Induction and carbon catabolite repression of phenol degradation genes in Rhodococcus erythropolis and Rhodococcus jostii (2014), Appl. Microbiol. Biotechnol., 98, 8267-8279.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
gene pheA1, quantitative real-time PCR-based expression and promoter activity analysis, recombinant expression in Corynebacterium glutamicum strain RES167, a heterologous host lacking the PheR regulator, coexpression of gene pheA1 with gene pheA2 and regulator gene pheR, subcloning in Escherichia coli strain DH5alpha Rhodococcus erythropolis
gene pheA1, quantitative real-time PCR-based expression and promoter activity analysis, recombinant expression in Corynebacterium glutamicum strain RES167, a heterologous host lacking the PheR regulator, coexpression of gene pheA1 with gene pheA2 and regulator gene pheR, subcloning in Escherichia coli strain DH5alpha Rhodococcus jostii

Organism

Organism UniProt Comment Textmining
Rhodococcus erythropolis A7LCL0 gene pheA1
-
Rhodococcus erythropolis CCM2595 A7LCL0 gene pheA1
-
Rhodococcus jostii Q0SDR7 gene pheA1
-

Expression

Organism Comment Expression
Rhodococcus erythropolis the activity of the key enzyme of the phenol degradation pathway, two-component phenol hydroxylase, encoded by genes pheA2-pheA1, is repressed substantially by succinate, quantitative RT-PCR reveals that expression of the phe genes is suppressed by succinate at the transcriptional level down
Rhodococcus erythropolis no repression by glucose or glycerol additional information
Rhodococcus erythropolis the activity of the key enzyme of the phenol degradation pathway, two-component phenol hydroxylase, is induced by phenol, quantitative RT-PCR reveals that expression of the phe genes is induced by phenol at the transcriptional level up

General Information

General Information Comment Organism
metabolism the gene cluster comprising genes catA, catB, catC, catR, pheR, pheA2, pheA1, is involved in the ortho-cleavage pathway of phenol, the key enzyme of the phenol degradation pathway is the two-component phenol hydroxylase. Regulation analysis, the type of carbon catabolite repression and the temporal transcriptional pattern during cultivation are different in each of the analyzed phe clusters from Rhodococcus erythropolis and Rhodococcus jostii Rhodococcus erythropolis
metabolism the gene cluster comprising genes catA, catB, catC, catR, pheR, pheA2, pheA1, is involved in the ortho-cleavage pathway of phenol, the key enzyme of the phenol degradation pathway is the two-component phenol hydroxylase. Regulation analysis, the type of carbon catabolite repression and the temporal transcriptional pattern during cultivation are different in each of the analyzed phe clusters from Rhodococcus erythropolis and Rhodococcus jostii Rhodococcus jostii