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Literature summary for 1.14.14.21 extracted from

  • Konishi, J.; Ishii, Y.; Onaka, T.; Maruhashi, K.
    Purification and characterization of the monooxygenase catalyzing sulfur-atom specific oxidation of dibenzothiophene and benzothiophene from the thermophilic bacterium Paenibacillus sp. strain A11-2 (2003), Appl. Microbiol. Biotechnol., 60, 128-133.
    View publication on PubMed
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Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline complete inhibition at 1 mM Paenibacillus sp.
5,5'-dithio-bis-2-nitrobenzoic acid 94% inhibition at 0.5 mM Paenibacillus sp.
CaCl2 16% inhibition at 1 mM Paenibacillus sp.
CoCl2 74% inhibition at 1 mM Paenibacillus sp.
CuCl2 complete inhibition at 1 mM Paenibacillus sp.
EDTA 68% inhibition at 1 mM Paenibacillus sp.
iodoacetate 46% inhibition at 1 mM Paenibacillus sp.
MnCl2 66% inhibition at 1 mM Paenibacillus sp.
N-ethylmaleimide 98% inhibition at 1 mM Paenibacillus sp.
p-chloromercuribenzoic acid complete inhibition at 0.5 mM Paenibacillus sp.
ZnCl2 complete inhibition at 1 mM Paenibacillus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.36
-
 benzothiophene pH 7.0, 55°C Paenibacillus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
 4 * 43000, SDS-PAGE Paenibacillus sp.
200000
-
 gel filtration Paenibacillus sp.

Organism

Organism UniProt Comment Textmining
Paenibacillus sp. Q9LBX2
-
-
Paenibacillus sp. A11-2 Q9LBX2
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 116fold from strain All-2 by two different steps of anion exchange chromatography and dialysis, followed by hydrophobic interaction and hydroxyapatite chromatography Paenibacillus sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
18.6
-
 purified enzyme, pH 7.0, 55°C Paenibacillus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-methyldibenzothiophene + 2 FMNH2 + 2 O2
-
 Paenibacillus sp. 2-methyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O
-
 ?
3-methylbenzothiophene + 2 FMNH2 + 2 O2
-
 Paenibacillus sp. 3-methylbenzothiophene-1,1-dioxide + 2 FMN + 2 H2O
-
 ?
3-methyldibenzothiophene + 2 FMNH2 + 2 O2
-
 Paenibacillus sp. 3-methyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O
-
 ?
4,6-diethyldibenzothiophene + 2 FMNH2 + 2 O2
-
 Paenibacillus sp. 4,6-diethyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O
-
 ?
4,6-dimethyldibenzothiophene + 2 FMNH2 + 2 O2 low activity Paenibacillus sp. 4,6-dimethyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O
-
 ?
4,6-dimethyldibenzothiophene + 2 FMNH2 + 2 O2 low activity Paenibacillus sp. A11-2 4,6-dimethyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O
-
 ?
4-methyldibenzothiophene + 2 FMNH2 + 2 O2 low activity Paenibacillus sp. 4-methyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O
-
 ?
4-methyldibenzothiophene + 2 FMNH2 + 2 O2 low activity Paenibacillus sp. A11-2 4-methyldibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O
-
 ?
5-methylbenzothiophene + 2 FMNH2 + 2 O2
-
 Paenibacillus sp. 5-methylbenzothiophene-5,5-dioxide + 2 FMN + 2 H2O
-
 ?
benzothiophene + 2 FMNH2 + 2 O2 best substrate Paenibacillus sp. benzothiophene-5,5-dioxide + 2 FMN + 2 H2O
-
 ?
benzothiophene + 2 FMNH2 + 2 O2 best substrate Paenibacillus sp. A11-2 benzothiophene-5,5-dioxide + 2 FMN + 2 H2O
-
 ?
dibenzothiophene + 2 FMNH2 + 2 O2 low reactivity of TdsC toward dibenzothiophene at high substrate concentrations Paenibacillus sp. dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O
-
 ?
dibenzothiophene + 2 FMNH2 + 2 O2 low reactivity of TdsC toward dibenzothiophene at high substrate concentrations Paenibacillus sp. A11-2 dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O
-
 ?
additional information substrate specificity of enzyme TsdC with dibenzothiophene derivatives and benzothiophene derivatives, overview. No or poor activity with 4,6-dipropyldibenzothiophene, 4,6-dibutyldibenzothiophene, and 1-methyldibenzothiophene Paenibacillus sp. ?
-
 ?
additional information substrate specificity of enzyme TsdC with dibenzothiophene derivatives and benzothiophene derivatives, overview. No or poor activity with 4,6-dipropyldibenzothiophene, 4,6-dibutyldibenzothiophene, and 1-methyldibenzothiophene Paenibacillus sp. A11-2 ?
-
 ?

Subunits

Subunits Comment Organism
homotetramer 4 * 43000, SDS-PAGE Paenibacillus sp.

Synonyms

Synonyms Comment Organism
benzothiophene monooxygenase
-
 Paenibacillus sp.
BT monooxygenase
-
 Paenibacillus sp.
DBT monooxygenase
-
 Paenibacillus sp.
dibenzothiophene monooxygenase
-
 Paenibacillus sp.
TdsC
-
 Paenibacillus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
 Paenibacillus sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 65 activity range Paenibacillus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
 purified enzyme, retains more than 80% of its activity after exposure for 30 min Paenibacillus sp.
70
-
 purified enzyme, no activity remaining after 30 min Paenibacillus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Paenibacillus sp.

Cofactor

Cofactor Comment Organism Structure
FMNH2
-
 Paenibacillus sp.
additional information FAD and lumiflavin have only weak activity as reducing agents Paenibacillus sp.
additional information FMN oxidoreductase TdsD, a NADH-dependent FMN oxidoreductase, absolutely required for TdsC activity, maximum activity is obtained at a TdsD/TdsC molar ratio of 0.5, no activity in the absence of TdsD Paenibacillus sp.
NADH
-
 Paenibacillus sp.
NADPH can substitute for NADH to some extent Paenibacillus sp.