Cloned (Comment) | Organism |
---|---|
gene bdsC, recombinant expression in Escherichia coli strain BL21(DE3)/pET21-a/bdsC | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dibenzothiophene + 2 FMNH2 + 2 O2 | Bacillus subtilis | - |
dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
dibenzothiophene + 2 FMNH2 + 2 O2 | Bacillus subtilis WU-S2B | - |
dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
gene bdsC | - |
Bacillus subtilis WU-S2B | - |
gene bdsC | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,7-diethylbenzothiophene + FMNH2 + O2 | 13% of the activity with dibenzothiophene | Bacillus subtilis | ? | - |
? | |
2,7-diethylbenzothiophene + FMNH2 + O2 | 13% of the activity with dibenzothiophene | Bacillus subtilis WU-S2B | ? | - |
? | |
2-ethylbenzothiophene + FMNH2 + O2 | 28% of the activity with dibenzothiophene | Bacillus subtilis | ? | - |
? | |
2-ethylbenzothiophene + FMNH2 + O2 | 28% of the activity with dibenzothiophene | Bacillus subtilis WU-S2B | ? | - |
? | |
2-methylbenzothiophene + FMNH2 + O2 | 209% of the activity with dibenzothiophene | Bacillus subtilis | ? | - |
? | |
2-methylbenzothiophene + FMNH2 + O2 | 209% of the activity with dibenzothiophene | Bacillus subtilis WU-S2B | ? | - |
? | |
3-methylbenzothiophene + FMNH2 + O2 | less than 10% of the activity with dibenzothiophene | Bacillus subtilis | ? | - |
? | |
5-methylbenzothiophene + FMNH2 + O2 | 33% of the activity with dibenzothiophene | Bacillus subtilis | ? | - |
? | |
7-ethylbenzothiophene + FMNH2 + O2 | less than 10% of the activity with dibenzothiophene | Bacillus subtilis | ? | - |
? | |
dibenzothiophene + 2 FMNH2 + 2 O2 | - |
Bacillus subtilis | dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
dibenzothiophene + 2 FMNH2 + 2 O2 | - |
Bacillus subtilis WU-S2B | dibenzothiophene-5,5-dioxide + 2 FMN + 2 H2O | - |
? | |
additional information | the enzyme utilizes aromatic compounds, not having sulfur atoms, as substrates. It acts on indole, converting it to indoxyl and isatin, and its derivatives to form indigoid pigments, and also utilizes indoline and phenoxazine. Indigo derivative formation by BdsC using methylindoles as substrates. Enzyme BdsC exhibits activity toward benzothiophene derivatives but not benzothiophene itself showing a wide reactivity toward aromatic compounds. To measure BdsC activity, flavin reductase (DszD) from another desulfurizing species, Rhodococcus erythropolis strain D-1, is used. Sulfone formations, product analysis by GC-MS | Bacillus subtilis | ? | - |
? | |
additional information | the enzyme utilizes aromatic compounds, not having sulfur atoms, as substrates. It acts on indole, converting it to indoxyl and isatin, and its derivatives to form indigoid pigments, and also utilizes indoline and phenoxazine. Indigo derivative formation by BdsC using methylindoles as substrates. Enzyme BdsC exhibits activity toward benzothiophene derivatives but not benzothiophene itself showing a wide reactivity toward aromatic compounds. To measure BdsC activity, flavin reductase (DszD) from another desulfurizing species, Rhodococcus erythropolis strain D-1, is used. Sulfone formations, product analysis by GC-MS | Bacillus subtilis WU-S2B | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BdsC | - |
Bacillus subtilis |
dibenzothiophene monooxygenase | - |
Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Bacillus subtilis |
General Information | Comment | Organism |
---|---|---|
metabolism | the dibenzothiophene (DBT) desulfurization enzyme system consists of three enzymes: DBT monooxygenase, DBT sulfone monooxygenase, and 2'-hydroxybiphenyl 2-sulfinate desulfinase | Bacillus subtilis |