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Literature summary for 1.14.14.22 extracted from

  • Kayser, K.J.; Cleveland, L.; Park, H.S.; Kwak, J.H.; Kolhatkar, A.; Kilbane, J.J.
    Isolation and characterization of a moderate thermophile, Mycobacterium phlei GTIS10, capable of dibenzothiophene desulfurization (2002), Appl. Microbiol. Biotechnol., 59, 737-745.
    View publication on PubMed
Search for more literature for 1.14.14.22

Cloned(Commentary)

Cloned (Comment) Organism
gene dszA, DNA and amino acid sequence determination and analysis, sequence comparison, recombinant expression of His-tagged enzyme in Escherichia coli strain DH5alpha Mycolicibacterium phlei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2 Mycolicibacterium phlei
-
 2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O
-
 ?
dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2 Mycolicibacterium phlei GTIS10
-
 2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O
-
 ?
additional information Mycolicibacterium phlei The dszA gene encodes DBT-5,5-dioxide monooxygenase, which catalyzes the conversion of dibenzothiophene sulfone to 2'-hydroxybiphenyl-2-sulfinate ?
-
 ?
additional information Mycolicibacterium phlei GTIS10 The dszA gene encodes DBT-5,5-dioxide monooxygenase, which catalyzes the conversion of dibenzothiophene sulfone to 2'-hydroxybiphenyl-2-sulfinate ?
-
 ?

Organism

Organism UniProt Comment Textmining
Mycolicibacterium phlei
-
-
-
Mycolicibacterium phlei GTIS10
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain DH5alpha by nickel affinty chromatography Mycolicibacterium phlei

Source Tissue

Source Tissue Comment Organism Textmining
cell culture the organism can use dibenzothiophene as a sole source of sulfur for growth at 30-52°C, it can also utilize benzothiophene and thiophene as sulfur sources for growth. Range of organosulfur substrates utilized as a sole source of sulfur for growth by Mycobacterium phlei GTIS10, best is dibenzothiophene, overview Mycolicibacterium phlei
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2
-
 Mycolicibacterium phlei 2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O
-
 ?
dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2
-
 Mycolicibacterium phlei GTIS10 2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O
-
 ?
additional information The dszA gene encodes DBT-5,5-dioxide monooxygenase, which catalyzes the conversion of dibenzothiophene sulfone to 2'-hydroxybiphenyl-2-sulfinate Mycolicibacterium phlei ?
-
 ?
additional information The dszA gene encodes DBT-5,5-dioxide monooxygenase, which catalyzes the conversion of dibenzothiophene sulfone to 2'-hydroxybiphenyl-2-sulfinate Mycolicibacterium phlei GTIS10 ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 assay at Mycolicibacterium phlei

Cofactor

Cofactor Comment Organism Structure
FMNH2
-
 Mycolicibacterium phlei

General Information

General Information Comment Organism
physiological function Mycobacterium phlei strain GTIS10 converts dibenzothiophene to 2'-hydroxybiphenyl, determination of metabolites from dibenzothiophene, overview Mycolicibacterium phlei