Literature summary for 1.14.14.22 extracted from

Ishii, Y.; Konishi, J.; Okada, H.; Hirasawa, K.; Onaka, T.; Suzuki, M.
Operon structure and functional analysis of the genes encoding thermophilic desulfurizing enzymes of Paenibacillus sp. A11-2 (2000), Biochem. Biophys. Res. Commun., 270, 81-88.

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Cloned(Commentary)

Cloned (Comment)	Organism
gene tdsA, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, recombinant expression in Escherichia coli, coexpression with genes tdsC and tdsB, leading to established thermophilic desulfurization of dibenzothiophene in Escherichia coli host, an Escherichia coli oxidoreductase can be functionally coupled with the monooxygenases of a gram-positive thermophile. The end of tdsA shows a 4-bp overlap with the translational start site of tdsB	Paenibacillus sp.

Cloned (Comment)

Organism

gene tdsA, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, recombinant expression in Escherichia coli, coexpression with genes tdsC and tdsB, leading to established thermophilic desulfurization of dibenzothiophene in Escherichia coli host, an Escherichia coli oxidoreductase can be functionally coupled with the monooxygenases of a gram-positive thermophile. The end of tdsA shows a 4-bp overlap with the translational start site of tdsB

Paenibacillus sp.

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
38900	-	x * 38900, about, sequnence calculation	Paenibacillus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2	Paenibacillus sp.	-	2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O	-	?
dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2	Paenibacillus sp. A11-2	-	2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Paenibacillus sp.	Q9LBX4	gene tdsA	-
Paenibacillus sp. A11-2	Q9LBX4	gene tdsA	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2	-	Paenibacillus sp.	2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O	-	?
dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2	-	Paenibacillus sp. A11-2	2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O	-	?

Subunits

Subunits	Comment	Organism
?	x * 38900, about, sequnence calculation	Paenibacillus sp.

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	50	assay at, cell extract	Paenibacillus sp.

Cofactor

Cofactor	Comment	Organism	Structure
FMNH2	-	Paenibacillus sp.