Crystallization (Comment) | Organism |
---|---|
purified enzyme DszA, hanging drop vapour diffusion method, using a reservoir suliton containing 12% polyethylene glycol 400 and 0.2 M CaCl2, 4°C, X-ray diffraction structure determination and analysis | Rhodococcus erythropolis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
2 * 50000, SDS-PAGE | Rhodococcus erythropolis |
97000 | - |
gel filtration | Rhodococcus erythropolis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2 | Rhodococcus erythropolis | - |
2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O | - |
? | |
dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2 | Rhodococcus erythropolis D-1 | - |
2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus erythropolis | Q6WE15 | gene dszA | - |
Rhodococcus erythropolis D-1 | Q6WE15 | gene dszA | - |
Purification (Comment) | Organism |
---|---|
native enzyme to homogeneity by anion exchange and hydrophobic interaction chromatography, and gel filtration | Rhodococcus erythropolis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2 | - |
Rhodococcus erythropolis | 2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O | - |
? | |
dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2 | best substrate | Rhodococcus erythropolis | 2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O | - |
? | |
dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2 | - |
Rhodococcus erythropolis D-1 | 2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O | - |
? | |
dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2 | best substrate | Rhodococcus erythropolis D-1 | 2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O | - |
? | |
dibenz[c,e][1,2]oxathiin 6,6-dioxide + 2 FMNH2 + O2 | i.e. sultone | Rhodococcus erythropolis | 2,2'-dihydroxybiphenyl + 2 FMN + H2O | - |
? | |
dibenz[c,e][1,2]oxathiin 6,6-dioxide + 2 FMNH2 + O2 | i.e. sultone | Rhodococcus erythropolis D-1 | 2,2'-dihydroxybiphenyl + 2 FMN + H2O | - |
? | |
additional information | no activity with dibenzothiophene and 2'-hydroxybiphenyl 2-sulfinic acid. DszA may recognize the sulfone moiety within the structure of DBT sulfone and sultone | Rhodococcus erythropolis | ? | - |
? | |
additional information | no activity with dibenzothiophene and 2'-hydroxybiphenyl 2-sulfinic acid. DszA may recognize the sulfone moiety within the structure of DBT sulfone and sultone | Rhodococcus erythropolis D-1 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 50000, SDS-PAGE | Rhodococcus erythropolis |
Synonyms | Comment | Organism |
---|---|---|
DBT sulfone monooxygenase | - |
Rhodococcus erythropolis |
dibenzothiophene sulfone monooxygenase | - |
Rhodococcus erythropolis |
dszA | - |
Rhodococcus erythropolis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Rhodococcus erythropolis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Rhodococcus erythropolis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMNH2 | - |
Rhodococcus erythropolis |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved in the pathway of microbial dibenzothiophene desulfurization, overview | Rhodococcus erythropolis |