Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no inhibition by EDTA | Rhodococcus erythropolis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Rhodococcus erythropolis | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme does not contain Fe2+ or Zn2+, no stimulation by Fe3+, Fe2+, or Cu2+, enzyme DszA does not require a metal cofactor | Rhodococcus erythropolis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49579 | - |
2 * 49579, sequence calculation | Rhodococcus erythropolis |
100000 | - |
gel filtration | Rhodococcus erythropolis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2 | Rhodococcus erythropolis | - |
2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O | - |
? | |
dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2 | Rhodococcus erythropolis IGTS8 / ATCC 53968 | - |
2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus erythropolis | - |
gene dszA | - |
Rhodococcus erythropolis IGTS8 / ATCC 53968 | - |
gene dszA | - |
Purification (Comment) | Organism |
---|---|
native enzyme from strain IGTS8 by anion exchange chromatography and gel filtration | Rhodococcus erythropolis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2 | - |
Rhodococcus erythropolis | 2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O | - |
? | |
dibenzothiophene-5,5-dioxide + 2 FMNH2 + O2 | - |
Rhodococcus erythropolis IGTS8 / ATCC 53968 | 2'-hydroxybiphenyl-2-sulfinate + 2 FMN + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 49579, sequence calculation | Rhodococcus erythropolis |
Synonyms | Comment | Organism |
---|---|---|
DBTO2-MO | - |
Rhodococcus erythropolis |
DBTO2-monooxygenase | - |
Rhodococcus erythropolis |
dszA | - |
Rhodococcus erythropolis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Rhodococcus erythropolis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Rhodococcus erythropolis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMNH2 | - |
Rhodococcus erythropolis | |
NADH | the enzyme does not directly react with NADH, but uses the activity of the FMN:NADH oxidoreductase, DszD | Rhodococcus erythropolis |
General Information | Comment | Organism |
---|---|---|
metabolism | strain IGTS8 has the ability to convert dibenzothiophene to 2-hydroxybiphenyl with the release of inorganic sulfur. The conversion of dibenzothiophene to 2-hydroxybiphenyl is catalyzed by a multienzyme pathway consisting of two monooxygenases and a desulfinase. The final reaction catalyzed by the desulfinase DszB appears to be the rate limiting step in the pathway | Rhodococcus erythropolis |