BRENDA - Enzyme Database show
show all sequences of 1.14.14.23

Sulfhydryl groups responsible for extreme lability of human cholesterol 7alpha-hydroxylase identified by site-directed mutagenesis

Tanaka, S.; Kinowaki, M.; Eto, T.A.; Ota, Y.; Maeda, Y.; Okuda, K.; Setoguchi, T.; Chijiiwa, K.; J. Steroid Biochem. Mol. Biol. 86, 35-40 (2003)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
native and mutated genes expressed in COS-1 cells
Homo sapiens
Engineering
Amino acid exchange
Commentary
Organism
C16A
strongly decreased activity
Homo sapiens
C175A
decreased activity
Homo sapiens
C17A
increased activity
Homo sapiens
C444A
no activity
Homo sapiens
C44A
decreased activity
Homo sapiens
C476A
no activity
Homo sapiens
C69A
no effect on activity
Homo sapiens
C90A
decreased activity
Homo sapiens
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Homo sapiens
-
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
58000
-
x * 58000, SDS-PAGE
Homo sapiens
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
cholesterol + [reduced NADPH-hemoprotein reductase] + O2
Cricetulus griseus
first step of cholesterol catabolism
7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
cholesterol + [reduced NADPH-hemoprotein reductase] + O2
Mus musculus
first step of cholesterol catabolism
7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
cholesterol + [reduced NADPH-hemoprotein reductase] + O2
Homo sapiens
first step of cholesterol catabolism
7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
cholesterol + [reduced NADPH-hemoprotein reductase] + O2
Rattus norvegicus
first step of cholesterol catabolism
7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
cholesterol + [reduced NADPH-hemoprotein reductase] + O2
Oryctolagus cuniculus
first step of cholesterol catabolism
7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Cricetulus griseus
-
-
-
Homo sapiens
-
-
-
Mus musculus
-
-
-
Oryctolagus cuniculus
-
-
-
Rattus norvegicus
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
cholesterol + [reduced NADPH-hemoprotein reductase] + O2
-
659809
Cricetulus griseus
7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
cholesterol + [reduced NADPH-hemoprotein reductase] + O2
-
659809
Mus musculus
7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
cholesterol + [reduced NADPH-hemoprotein reductase] + O2
-
659809
Homo sapiens
7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
cholesterol + [reduced NADPH-hemoprotein reductase] + O2
-
659809
Rattus norvegicus
7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
cholesterol + [reduced NADPH-hemoprotein reductase] + O2
-
659809
Oryctolagus cuniculus
7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
cholesterol + [reduced NADPH-hemoprotein reductase] + O2
first step of cholesterol catabolism
659809
Cricetulus griseus
7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
cholesterol + [reduced NADPH-hemoprotein reductase] + O2
first step of cholesterol catabolism
659809
Mus musculus
7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
cholesterol + [reduced NADPH-hemoprotein reductase] + O2
first step of cholesterol catabolism
659809
Homo sapiens
7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
cholesterol + [reduced NADPH-hemoprotein reductase] + O2
first step of cholesterol catabolism
659809
Rattus norvegicus
7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
cholesterol + [reduced NADPH-hemoprotein reductase] + O2
first step of cholesterol catabolism
659809
Oryctolagus cuniculus
7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 58000, SDS-PAGE
Homo sapiens
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Cricetulus griseus
NADPH
-
Oryctolagus cuniculus
NADPH
-
Rattus norvegicus
NADPH
-
Mus musculus
NADPH
-
Homo sapiens
Cloned(Commentary) (protein specific)
Commentary
Organism
native and mutated genes expressed in COS-1 cells
Homo sapiens
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Cricetulus griseus
NADPH
-
Oryctolagus cuniculus
NADPH
-
Rattus norvegicus
NADPH
-
Mus musculus
NADPH
-
Homo sapiens
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
C16A
strongly decreased activity
Homo sapiens
C175A
decreased activity
Homo sapiens
C17A
increased activity
Homo sapiens
C444A
no activity
Homo sapiens
C44A
decreased activity
Homo sapiens
C476A
no activity
Homo sapiens
C69A
no effect on activity
Homo sapiens
C90A
decreased activity
Homo sapiens
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Homo sapiens
-
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
58000
-
x * 58000, SDS-PAGE
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
cholesterol + [reduced NADPH-hemoprotein reductase] + O2
Cricetulus griseus
first step of cholesterol catabolism
7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
cholesterol + [reduced NADPH-hemoprotein reductase] + O2
Mus musculus
first step of cholesterol catabolism
7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
cholesterol + [reduced NADPH-hemoprotein reductase] + O2
Homo sapiens
first step of cholesterol catabolism
7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
cholesterol + [reduced NADPH-hemoprotein reductase] + O2
Rattus norvegicus
first step of cholesterol catabolism
7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
cholesterol + [reduced NADPH-hemoprotein reductase] + O2
Oryctolagus cuniculus
first step of cholesterol catabolism
7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
cholesterol + [reduced NADPH-hemoprotein reductase] + O2
-
659809
Cricetulus griseus
7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
cholesterol + [reduced NADPH-hemoprotein reductase] + O2
-
659809
Mus musculus
7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
cholesterol + [reduced NADPH-hemoprotein reductase] + O2
-
659809
Homo sapiens
7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
cholesterol + [reduced NADPH-hemoprotein reductase] + O2
-
659809
Rattus norvegicus
7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
cholesterol + [reduced NADPH-hemoprotein reductase] + O2
-
659809
Oryctolagus cuniculus
7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
cholesterol + [reduced NADPH-hemoprotein reductase] + O2
first step of cholesterol catabolism
659809
Cricetulus griseus
7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
cholesterol + [reduced NADPH-hemoprotein reductase] + O2
first step of cholesterol catabolism
659809
Mus musculus
7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
cholesterol + [reduced NADPH-hemoprotein reductase] + O2
first step of cholesterol catabolism
659809
Homo sapiens
7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
cholesterol + [reduced NADPH-hemoprotein reductase] + O2
first step of cholesterol catabolism
659809
Rattus norvegicus
7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
cholesterol + [reduced NADPH-hemoprotein reductase] + O2
first step of cholesterol catabolism
659809
Oryctolagus cuniculus
7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 58000, SDS-PAGE
Homo sapiens
Other publictions for EC 1.14.14.23
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
735897
Krishnamurthy
Heat shock factor-1 knockout e ...
Mus musculus
Cardiovasc. Res.
111
74-83
2016
-
-
1
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
2
-
-
-
-
-
-
-
-
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1
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1
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2
-
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-
-
-
-
-
-
1
3
3
1
-
-
735690
Frank
Cytochrome P450 125A4, the thi ...
Mycolicibacterium smegmatis
Biochemistry
54
6909-6916
2015
-
-
-
-
-
-
-
-
-
-
-
1
-
3
-
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1
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1
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1
-
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1
-
-
1
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
736169
Chow
Vitamin D receptor activation ...
Homo sapiens, Mus musculus, Mus musculus C57BL/6
Gastroenterology
146
1048-1059
2014
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
-
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-
-
7
-
-
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-
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-
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-
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
2
4
4
2
-
-
736605
Tempel
Structural characterization of ...
Homo sapiens
J. Lipid Res.
55
1925-1932
2014
-
-
-
1
1
-
-
-
-
-
-
1
-
3
-
-
-
-
-
1
-
-
6
-
1
-
-
7
1
-
-
2
-
-
-
-
-
-
2
1
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
6
-
1
-
-
7
1
-
-
-
-
3
3
-
-
-
736606
Fu
Atorvastatin induces bile acid ...
Mus musculus, Mus musculus C57BL/6
J. Lipid Res.
55
2576-2586
2014
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
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-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
1
1
1
1
-
-
736800
Lammel Lindemann
Thyroid hormone induction of h ...
Homo sapiens
Mol. Cell. Endocrinol.
388
32-40
2014
-
1
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
1
3
3
1
-
-
725114
Li
Regulation of cholesterol and ...
Homo sapiens, Rattus norvegicus
Hepatology
58
1111-1121
2013
-
1
2
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
1
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-
1
2
1
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-
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-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
725766
Kawase
Increased effects of ginsenosi ...
Rattus norvegicus
J. Nat. Med.
67
545-553
2013
-
1
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
1
-
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1
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1
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1
-
-
1
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-
723941
Michelini
Dual-color bioluminescent assa ...
Homo sapiens
Anal. Biochem.
430
92-96
2012
-
2
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
1
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2
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1
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-
-
-
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-
1
-
-
1
-
-
725113
Li
Overexpression of cholesterol ...
Rattus norvegicus
Hepatology
53
996-1006
2011
-
-
1
-
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-
-
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-
4
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1
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1
1
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725442
Shinkyo
Conversion of 7-dehydrocholest ...
Homo sapiens
J. Biol. Chem.
286
33021-33028
2011
-
1
-
-
-
-
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3
1
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2
-
1
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1
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3
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3
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1
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3
1
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2
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1
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3
-
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-
-
3
-
-
-
-
-
-
-
-
3
3
700653
Kovar
Regulation of diurnal variatio ...
Homo sapiens
Physiol. Res.
59
233-238
2010
-
-
-
-
-
-
-
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-
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2
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711777
De Castro-Oros
Promoter variant -204A>C of th ...
Homo sapiens
Clin. Nutr.
30
239-246
2010
-
-
1
-
1
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3
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1
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1
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-
-
-
-
-
-
-
-
2
2
-
-
-
712117
Wooton-Kee
Mechanisms for increased expre ...
Rattus norvegicus
Hepatology
51
277-285
2010
-
-
-
-
-
-
1
-
-
-
-
-
-
3
-
-
-
-
-
4
-
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1
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4
-
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-
-
-
-
-
-
-
-
-
-
2
2
2
2
-
-
712118
Li
Transgenic expression of chole ...
Mus musculus, Mus musculus C57BL/6
Hepatology
52
678-690
2010
-
-
-
-
1
-
-
-
1
-
-
-
-
143
-
-
-
-
-
4
-
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1
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1
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4
-
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-
-
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2
2
-
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-
712675
Song
A putative role of micro RNA i ...
Homo sapiens
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Glucose stimulates cholesterol ...
Homo sapiens
J. Lipid Res.
51
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2010
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2
2
2
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695480
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Hepatic bile acid metabolism i ...
Mesocricetus auratus
Am. J. Physiol. Gastrointest. Liver Physiol.
297
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2009
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1
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1
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696027
Kojima
IL-1 regulates the Cyp7a1 gene ...
Mus musculus
Biochem. Biophys. Res. Commun.
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2009
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696446
Li
Forkhead box transcription fac ...
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Biochim. Biophys. Acta
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2009
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2
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2
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697591
Han
Mechanism of vitamin D recepto ...
Homo sapiens
Drug Metab. Dispos.
37
469-478
2009
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1
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1
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698159
Song
Bile acids activate fibroblast ...
Homo sapiens
Hepatology
49
297-305
2009
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698904
Xie
Decreased expression of choles ...
Mus musculus
J. Biol. Chem.
284
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2009
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700216
Zhang
Significance and mechanism of ...
Mus musculus
Mol. Endocrinol.
23
137-145
2009
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700572
Zhou
Decreased protein and gene exp ...
Rattus norvegicus
Pathol. Int.
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729-734
2009
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685861
Lee
Green tea catechin enhances ch ...
Homo sapiens
Br. J. Nutr.
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2008
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686834
Hubacek
Differences in expression of c ...
Rattus norvegicus
Folia Biol. (Praha)
54
18-23
2008
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687259
Cheng
Effects of wheat antioxidants ...
Rattus norvegicus
J. Agric. Food Chem.
56
5033-5042
2008
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3
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688228
Li
TGF{beta}1, TNF{alpha}, and in ...
Rattus norvegicus
J. Lipid Res.
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2008
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689029
Srivastava
Role of genetic variant A-204C ...
Homo sapiens
Mol. Genet. Metab.
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83-89
2008
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695473
Wooton-Kee
Increased cholesterol 7alpha-h ...
Rattus norvegicus
Am. J. Physiol. Gastrointest. Liver Physiol.
294
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2008
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1
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1
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1
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698408
Nass
Screening for nutritive peptid ...
Homo sapiens
J. Agric. Food Chem.
56
4987-4994
2008
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2
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1
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1
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699703
Taniguchi
Cholesterol lowering effect of ...
Rattus norvegicus
J. Nutr. Sci. Vitaminol.
54
448-453
2008
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1
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-
-
-
-
-
-
-
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1
-
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1
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-
699708
Pond
Neonatal dietary cholesterol a ...
Sus scrofa
J. Nutr.
138
282-286
2008
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1
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673917
Wang
Differential hepatocellular zo ...
Mus musculus
Histochem. Cell Biol.
127
253-261
2007
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1
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2
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1
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1
1
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1
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2
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1
-
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685674
Shibata
Hypocholesterolemic mechanism ...
Rattus norvegicus
Biosci. Biotechnol. Biochem.
71
916-925
2007
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687007
Mitro
Insights in the regulation of ...
Homo sapiens
Hepatology
46
885-897
2007
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1
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687829
Noshiro
Multiple mechanisms regulate c ...
Mus musculus
J. Biol. Rhythms
22
299-311
2007
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3
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3
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689381
Gilardi
The pharmacological exploitati ...
Homo sapiens, Mus musculus
Pharmacol. Ther.
116
449-472
2007
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2
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674652
Li
Insulin regulation of choleste ...
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J. Biol. Chem.
281
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2006
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1
1
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1
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1
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2
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2
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675996
Hubacek
Role of cholesterol 7alpha-hyd ...
Homo sapiens
Mol. Diagn. Ther.
10
93-100
2006
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1
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4
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2
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1
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1
1
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4
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1
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1
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2
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667150
Shin
Effects of gender on hepatic H ...
Rattus norvegicus
Am. J. Physiol. Endocrinol. Metab.
289
E993-E998
2005
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6
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1
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1
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1
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2
1
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6
-
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671341
Maeda
Bile acid biosynthesis during ...
Rattus norvegicus
Ann. Clin. Lab. Sci.
35
323-328
2005
-
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2
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1
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1
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1
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1
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3
7
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2
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1
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1
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671582
Handschin
Species-specific mechanisms fo ...
Gallus gallus
Arch. Biochem. Biophys.
434
75-85
2005
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1
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2
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2
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1
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1
1
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1
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1
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1
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2
-
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2
-
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-
-
-
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-
-
-
-
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-
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-
671675
Abrahamsson
Common polymorphisms in the CY ...
Homo sapiens
Atherosclerosis
182
37-45
2005
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1
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1
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1
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2
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3
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1
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1
1
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1
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1
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2
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2
-
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3
-
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-
-
-
-
-
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-
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-
671693
Stroup
Cholesterol 7alpha-hydroxylase ...
Homo sapiens
Biochem. Biophys. Res. Commun.
329
957-965
2005
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1
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1
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1
2
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1
1
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