Cloned (Comment) | Organism |
---|---|
expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3), stable expression of CYP7A cDNA using the cytomegalovirus promoter and the bovine growth hormone polyadenylation signal in Hep-G2 cells | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
taurochenodeoxycholate | 70% inhibition at 0.1 mM | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Homo sapiens | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
x * 50000, approximately, SDS-PAGE | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesterol + [reduced NADPH-hemoprotein reductase] + O2 | Homo sapiens | - |
7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P22680 | gene CYP7A1 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | the enzyme is phosphorylated at multiple amino acids, overview | Homo sapiens |
Purification (Comment) | Organism |
---|---|
native and recombinant enzyme partially by microsome preparation | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
Hep-G2 cell | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0000034 | - |
wild-type Hep-G2 cell microsomes | Homo sapiens |
0.000092 | - |
recombinant enzyme in transformed Hep-G2 cells | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cholesterol + [reduced NADPH-hemoprotein reductase] + O2 | - |
Homo sapiens | 7alpha-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 50000, approximately, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
cholesterol 7alpha-hydroxylase | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Homo sapiens |