Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli, wild-type and trucation mutatants. All four mutants lack the N-terminal transmembrane region (residues 327), and, in addition, DELTA46A1 has a 4 His-tag fused to the C-terminus, HDELTA46A1 has the N-terminal 4 His-tag, HDELTA46A1DELTA has a 4 His-tag at the N-terminus and does not contain a proline-rich region at the C-terminus (residues 494499), and DELTA46A1DELTA lacks the C-terminal proline-rich region | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | four truncation mutants: all lack the N-terminal transmembrane region (residues 3 27), and, in addition, DELTA46A1 has a 4 His-tag fused to the C-terminus, HDELTA46A1 has the N-terminal 4 His-tag, HDELTA46A1DELTA has a 4 His-tag at the N-terminus and does not contain a proline-rich region at the C-terminus (residues 494 499), and DELTA46A1DELTA lacks the C-terminal proline-rich region. Truncated enzymes have moderately decreased catalytic efficiencies for either cholesterol or 24S-hydroxycholesterol or both, whereas their substrate-binding constants are either unchanged or decreased 2fold.The two forms, DELTA64A1DELTA and HDELTA46A1DELTA both lacking the C-terminal proline-rich region are good candidates for future crystallographic studies because they contain only 0.3 0.8% of high molecular weight aggregates and their catalytic efficiencies are decreased no more than 2.3fold | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km-value of truncated enzyme forms | Homo sapiens | |
0.0022 | - |
(24S)-24-hydroxycholesterol | full length enzyme | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | truncated enzymes are distributed at about a 1:1 ratio between the membrane fraction and the cytosol in low ionic strength buffer, when expressed in Escherichia coli | Homo sapiens | 5829 | - |
membrane | truncated enzymes are distributed at about a 1:1 ratio between the membrane fraction and the cytosol in low ionic strength buffer, when expressed in Escherichia coli | Homo sapiens | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49000 | - |
1 * 49000, truncated enzyme form, SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
truncated enzyme forms | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(24S)-24-hydroxycholesterol + [reduced NADPH-hemoprotein reductase] + O2 | - |
Homo sapiens | 24,25-dihydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
(24S)-24-hydroxycholesterol + [reduced NADPH-hemoprotein reductase] + O2 | - |
Homo sapiens | 24,27-dihydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
cholesterol + [reduced NADPH-hemoprotein reductase] + H+ + O2 | - |
Homo sapiens | (24S)-24-hydroxycholesterol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 49000, truncated enzyme form, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
cholesterol 24-monooxygenase | - |
Homo sapiens |
cholesterol 24S-hydroxylase | - |
Homo sapiens |
CYP46 | - |
Homo sapiens |
CYP46A1 | - |
Homo sapiens |
cytochrome P-450 46A1 | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | turnover-numbers of truncated enzyme forms | Homo sapiens | |
0.00117 | - |
(24S)-24-hydroxycholesterol | full length enzyme | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome P-450 | - |
Homo sapiens | |
NADPH | - |
Homo sapiens |