Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
68000 | - |
gel filtration | Pseudomonas putida |
70000 | - |
1 * 70000, SDS-PAGE | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
resorcinol + FADH2 + O2 | Pseudomonas putida | the enzyme participates in the degradation of gamma-resorcylate and resorcinol | hydroxyquinol + FAD + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Pseudomonas putida ORC | - |
- |
- |
Purification (Comment) | Organism |
---|---|
resorcinol hydroxylase accounts for about 3% of the total protein in Pseudomonas putida ORC after growth on resorcinol | Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
11.6 | - |
pH 6.8, 30°C | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
orcinol + FADH2 + O2 | 90% of the hydroxylation activity as compared to resorcinol | Pseudomonas putida | ? + FAD + H2O | - |
? | |
resorcinol + FADH2 + O2 | - |
Pseudomonas putida | hydroxyquinol + FAD + H2O | - |
? | |
resorcinol + FADH2 + O2 | the enzyme participates in the degradation of gamma-resorcylate and resorcinol | Pseudomonas putida | hydroxyquinol + FAD + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 70000, SDS-PAGE | Pseudomonas putida |
Synonyms | Comment | Organism |
---|---|---|
graA | - |
Pseudomonas putida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
assay at | Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FADH2 | contains approximately 1 mol of FAD for each polypeptide chain. The enzyme uses FADH2 as a substrate rather than a cofactor. FADH2 is provided by flavin reductase (NADH) | Pseudomonas putida |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme participates in the degradation of gamma-resorcylate and resorcinol | Pseudomonas putida |