Any feedback?
Literature summary for 1.14.14.3 extracted from
- Bacterial luciferase: Assay, purification, and properties (1978), Methods Enzymol., 57, 135-152.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| citrate stabilizes against inactivation by proteases, heat, urea | Aliivibrio fischeri |
| citrate stabilizes against inactivation by proteases, heat, urea | Photobacterium phosphoreum |
| citrate stabilizes against inactivation by proteases, heat, urea | Vibrio harveyi |
| diphosphate stabilizes against inactivation by proteases, heat, urea | Aliivibrio fischeri |
| diphosphate stabilizes against inactivation by proteases, heat, urea | Photobacterium phosphoreum |
| diphosphate stabilizes against inactivation by proteases, heat, urea | Vibrio harveyi |
| inactivation by lyophilization | Aliivibrio fischeri |
| inactivation by lyophilization | Photobacterium phosphoreum |
| inactivation by lyophilization | Vibrio harveyi |
| labile to proteases | Aliivibrio fischeri |
| labile to proteases | Photobacterium phosphoreum |
| labile to proteases | Vibrio harveyi |
| no inactivation by repeated freezing/thawing | Aliivibrio fischeri |
| no inactivation by repeated freezing/thawing | Photobacterium phosphoreum |
| no inactivation by repeated freezing/thawing | Vibrio harveyi |
| phosphate stabilizes against inactivation by proteases, heat, urea | Aliivibrio fischeri |
| phosphate stabilizes against inactivation by proteases, heat, urea | Photobacterium phosphoreum |
| phosphate stabilizes against inactivation by proteases, heat, urea | Vibrio harveyi |
| sulfate stabilizes against inactivation by proteases, heat, urea | Aliivibrio fischeri |
| sulfate stabilizes against inactivation by proteases, heat, urea | Photobacterium phosphoreum |
| sulfate stabilizes against inactivation by proteases, heat, urea | Vibrio harveyi |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2,3-Dichloro-(6-phenylphenoxy)ethylamine | - |
Aliivibrio fischeri |  |
| 2,3-Dichloro-(6-phenylphenoxy)ethylamine | - |
Vibrio harveyi | |
| 2-diethylaminoethyl-2,2-diphenylvalerate | - |
Aliivibrio fischeri | |
| 2-diethylaminoethyl-2,2-diphenylvalerate | - |
Vibrio harveyi | |
| 8-Anilino-1-naphthalenesulfonate | - |
Aliivibrio fischeri | |
| 8-Anilino-1-naphthalenesulfonate | - |
Vibrio harveyi | |
| amino group reagents | - |
Aliivibrio fischeri | |
| amino group reagents | - |
Photobacterium phosphoreum | |
| amino group reagents | - |
Vibrio harveyi | |
| imidazole reagents | - |
Aliivibrio fischeri | |
| imidazole reagents | - |
Photobacterium phosphoreum | |
| imidazole reagents | - |
Vibrio harveyi | |
| sulfhydryl reagents | - |
Aliivibrio fischeri | |
| sulfhydryl reagents | - |
Photobacterium phosphoreum | |
| sulfhydryl reagents | - |
Vibrio harveyi | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| decanal + FMNH + O2 | Aliivibrio fischeri | - |
decanoic acid + FMN + H2O + light | - |
ir | |
| decanal + FMNH + O2 | Photobacterium phosphoreum | - |
decanoic acid + FMN + H2O + light | - |
ir | |
| decanal + FMNH + O2 | Vibrio harveyi | - |
decanoic acid + FMN + H2O + light | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aliivibrio fischeri | - |
formerly Photobacterium fischeri | - |
| Photobacterium phosphoreum | - |
- |
- |
| Vibrio harveyi | - |
formerly Beneckea harveyi | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Vibrio harveyi |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| decanal + FMNH + O2 | - |
Aliivibrio fischeri | decanoic acid + FMN + H2O + light | - |
ir | |
| decanal + FMNH + O2 | - |
Photobacterium phosphoreum | decanoic acid + FMN + H2O + light | - |
ir | |
| decanal + FMNH + O2 | - |
Vibrio harveyi | decanoic acid + FMN + H2O + light | - |
ir | |
| dodecanal + FMNH + O2 | - |
Aliivibrio fischeri | dodecanoic acid + FMN + H2O + light | - |
ir | |
| dodecanal + FMNH + O2 | - |
Photobacterium phosphoreum | dodecanoic acid + FMN + H2O + light | - |
ir | |
| dodecanal + FMNH + O2 | - |
Vibrio harveyi | dodecanoic acid + FMN + H2O + light | - |
ir | |
| additional information | aldehydes of chain-length 8 or more required | Aliivibrio fischeri | ? | - |
? | |
| additional information | aldehydes of chain-length 8 or more required | Photobacterium phosphoreum | ? | - |
? | |
| additional information | aldehydes of chain-length 8 or more required | Vibrio harveyi | ? | - |
? | |
| octanal + FMNH + O2 | - |
Aliivibrio fischeri | octanoic acid + FMN + H2O + light | - |
ir | |
| octanal + FMNH + O2 | - |
Photobacterium phosphoreum | octanoic acid + FMN + H2O + light | - |
ir | |
| octanal + FMNH + O2 | - |
Vibrio harveyi | octanoic acid + FMN + H2O + light | - |
ir | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
stable below | Aliivibrio fischeri |
| 35 | - |
stable below | Photobacterium phosphoreum |
| 35 | - |
stable below | Vibrio harveyi |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8.5 | - |
Aliivibrio fischeri |
| 6 | 9.5 | - |
Vibrio harveyi |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
