Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) and HEK-293T cells | Vibrio campbellii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
77000 | - |
2 * 77000, SDS-PAGE | Vibrio campbellii |
77800 | - |
2 * 77800, calculated from amino acid sequence | Vibrio campbellii |
158000 | - |
gel filtration | Vibrio campbellii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio campbellii | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
RCHO + FMNH2 + O2 | - |
Vibrio campbellii | RCOOH + FMN + H2O + hv | the enzyme is emitting blue-green light at 490 nm | ? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 77000, SDS-PAGE | Vibrio campbellii |
homodimer | 2 * 77800, calculated from amino acid sequence | Vibrio campbellii |
Synonyms | Comment | Organism |
---|---|---|
luciferase | - |
Vibrio campbellii |
Lux | - |
Vibrio campbellii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 37 | half-life of about 1020 min at 37°C. At 37°C, the native enzyme retains around 60% of the bioluminescence of the enzyme expressed at 30°C | Vibrio campbellii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Vibrio campbellii |