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Literature summary for 1.14.14.31 extracted from

  • Song, M.; Kim, A.C.; Gorzalski, A.J.; MacLean, M.; Young, S.; Ginzel, M.D.; Blomquist, G.J.; Tittiger, C.
    Functional characterization of myrcene hydroxylases from two geographically distinct Ips pini populations (2013), Insect Biochem. Mol. Biol., 43, 336-343.
    View publication on PubMed
Search for more literature for 1.14.14.31

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Sf9 cells Ips pini

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
61000
-
 x * 61000, calculated from amino acid sequence Ips pini

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
myrcene + NADPH + H+ + O2 Ips pini 87% yield of (-)-ipsdienol, 13% yield of (+)-ipsdienol ipsdienol + NADP+ + H2O
-
 ?
myrcene + NADPH + H+ + O2 Ips pini the enzyme strongly prefers myrcene over other substrates ipsdienol + NADP+ + H2O 80% yield of (-)-ipsdienol, 20% yield of (+)-ipsdienol ?

Organism

Organism UniProt Comment Textmining
Ips pini M1JEK6
-
-
Ips pini Q00G65
-
-

Source Tissue

Source Tissue Comment Organism Textmining
midgut
-
 Ips pini
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+)-alpha-pinene + NADPH + H+ + O2
-
 Ips pini verbenol + NADP+ + H2O
-
 ?
(-)-alpha-pinene + NADPH + H+ + O2
-
 Ips pini cis-verbenol + NADP+ + H2O
-
 ?
(-)-alpha-pinene + NADPH + H+ + O2
-
 Ips pini myrtenol + NADP+ + H2O
-
 ?
3-carene + NADPH + H+ + O2
-
 Ips pini ?
-
 ?
additional information no activity with alpha-phellandrene, (-)-beta-pinene, gamma-terpinene, or terpinolene Ips pini ?
-
 ?
myrcene + NADPH + H+ + O2 87% yield of (-)-ipsdienol, 13% yield of (+)-ipsdienol Ips pini ipsdienol + NADP+ + H2O
-
 ?
myrcene + NADPH + H+ + O2 the enzyme strongly prefers myrcene over other substrates Ips pini ipsdienol + NADP+ + H2O 80% yield of (-)-ipsdienol, 20% yield of (+)-ipsdienol ?
R-(+)-limonene + NADPH + H+ + O2
-
 Ips pini ?
-
 ?

Subunits

Subunits Comment Organism
? x * 61000, calculated from amino acid sequence Ips pini

Synonyms

Synonyms Comment Organism
CYP9T2
-
 Ips pini
CYP9T3
-
 Ips pini
myrcene hydroxylase
-
 Ips pini

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Ips pini

pI Value

Organism Comment pI Value Maximum pI Value
Ips pini calculated from amino acid sequence
-
 9

Expression

Organism Comment Expression
Ips pini CYP9T2 mRNA levels are not induced in adults exposed to myrcene-saturated atmosphere additional information