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Literature summary for 1.14.14.32 extracted from

  • Peng, H.M.; Im, S.C.; Pearl, N.M.; Turcu, A.F.; Rege, J.; Waskell, L.; Auchus, R.J.
    Cytochrome b5 Activates the 17,20-lyase activity of human cytochrome P450 17A1 by increasing the coupling of NADPH consumption to androgen production (2016), Biochemistry, 55, 4356-4365.
    View publication on PubMedView publication on EuropePMC
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Activating Compound

Activating Compound Comment Organism Structure
cytochrome b5 presence of cytochrome b5 markedly stimulates the 17,20-lyase reaction, with little effect on 17-hydroxylation. For the 17-hydroxylase reaction, progesterone oxidation is the most tightly coupled (about 50%) and negligibly changed upon addition of b5. For the 17,20-lyase reactions, b5 markedly increases product formation and coupling in parallel with all substrates, from 6% to 44% with the major substrate 17-hydroxypregnenolone Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
-
 Homo sapiens

Protein Variants

Protein Variants Comment Organism
E305G naturally occurring P450 17A1 mutation, impairs 17,20-lyase activity. Cytochrome b5 fails to rescue the poor coupling with 17-hydroxypregnenolone Homo sapiens
R347H naturally occurring P450 17A1 mutation, impairs 17,20-lyase activity. Cytochrome b5 fails to rescue the poor coupling with 17-hydroxypregnenolone Homo sapiens
T306A mutation in the conserved active-site threonine, both the activity and coupling are markedly decreased with all substrates Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P05093
-
-

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
17alpha-hydroxypregnenolone + [reduced NADPH-hemoprotein reductase] + O2
-
 Homo sapiens 3beta-hydroxyandrost-5-en-17-one + acetate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
17alpha-hydroxyprogesterone + [reduced NADPH-hemoprotein reductase] + O2
-
 Homo sapiens androstenedione + acetate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
allopregnanolone + [reduced NADPH-hemoprotein reductase] + O2
-
 Homo sapiens androstenedione + acetate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Synonyms

Synonyms Comment Organism
cytochrome P450 17A1
-
 Homo sapiens