KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0085 | - |
ethylenediaminetetraacetate | presence of Mg2+, pH 7.8, 35°C | EDTA-degrading bacterium BNC1 | |
0.034 | - |
ethylenediaminetetraacetate | no addition of divalent ions, pH 7.8, 35°C | EDTA-degrading bacterium BNC1 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | MgEDTA2- is the best substrate | EDTA-degrading bacterium BNC1 | |
additional information | the enzyme is able to release glyoxylate from EDTA complexed with any of the cations tested (Mg2+, Mn2+, Ni2+, Co2+, Zn2+, Fe2+, Ca2+, Cu2+, Cr2+, Sn2+, Ba2+, Cd2+, Sr2+, Pd2+, Al3+, Cr3+, K+, or Na+). MgEDTA2- is the best substrate | EDTA-degrading bacterium BNC1 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
gel filtration | EDTA-degrading bacterium BNC1 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
EDTA-degrading bacterium BNC1 | Q9F9T3 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
EDTA-degrading bacterium BNC1 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diethylenetriaminepentaacetate + FMNH2 + O2 | - |
EDTA-degrading bacterium BNC1 | diethylenetriaminetetraacetate + glyoxylate + FMN + H2O | - |
? | |
ethylenediaminetetraacetate + 2 FMNH2 + 2 O2 | - |
EDTA-degrading bacterium BNC1 | ethylenediamine-N,N'-diacetate + 2 glyoxylate + 2 FMN + 2 H2O | - |
? | |
additional information | the enzyme oxidizes both EDTA complexed with various metal ions and uncomplexed EDTA | EDTA-degrading bacterium BNC1 | ? | - |
? | |
nitrilotriacetate + FMNH2 + O2 | - |
EDTA-degrading bacterium BNC1 | nitrilodiacetate + glyoxylate + FMN + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 45000, SDS-PAGE | EDTA-degrading bacterium BNC1 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
EDTA-degrading bacterium BNC1 |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
75% of maximum activity | EDTA-degrading bacterium BNC1 |
40 | - |
96% of maximum activity | EDTA-degrading bacterium BNC1 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.8 | - |
ethylenediaminetetraacetate | no addition of divalent ions, pH 7.8, 35°C | EDTA-degrading bacterium BNC1 | |
3.6 | - |
ethylenediaminetetraacetate | presence of Mg2+, pH 7.8, 35°C | EDTA-degrading bacterium BNC1 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.7 | - |
- |
EDTA-degrading bacterium BNC1 |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.9 | - |
75% of maximum activity | EDTA-degrading bacterium BNC1 |
8.1 | - |
70% of maximum activity | EDTA-degrading bacterium BNC1 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMNH2 | - |
EDTA-degrading bacterium BNC1 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
80 | - |
ethylenediaminetetraacetate | no addition of divalent ions, pH 7.8, 35°C | EDTA-degrading bacterium BNC1 | |
430 | - |
ethylenediaminetetraacetate | presence of Mg2+, pH 7.8, 35°C | EDTA-degrading bacterium BNC1 |