Crystallization (Comment) | Organism |
---|---|
to 2.15 A resolution. The structure shows a domain-like insertion into a TIM-barrel, which might serve as a flexible lid for the active site. Docking of MgEDTA2- into EmoA identifies an intricate hydrogen bond network connected to Tyr71, which should lower its pKa. Tyr71, along with nearby Glu70 and a peroxy flavin, facilitates a keto-enol transition of the leaving acetyl group of EDTA. The interaction between EmoA and oxidoreductase EmoB enhances both EmoA and EmoB activities probably through coupled channelling of FMNH2 | EDTA-degrading bacterium BNC1 |
Protein Variants | Comment | Organism |
---|---|---|
E70A | complete loss of activtiy | EDTA-degrading bacterium BNC1 |
P291A | 74.4% of wild-type activity | EDTA-degrading bacterium BNC1 |
Q294A | 85.6% of wild-type activity | EDTA-degrading bacterium BNC1 |
Q58A | 51.3% of wild-type activity | EDTA-degrading bacterium BNC1 |
R68A | 85.3% of wild-type activity | EDTA-degrading bacterium BNC1 |
W11A | 26.4% of wild-type activity | EDTA-degrading bacterium BNC1 |
Y71A | complete loss of activtiy | EDTA-degrading bacterium BNC1 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0461 | - |
ethylenediaminetetraacetate | wild-type, presence of Mg2+ and oxidoreductase EmoB, pH 7.6, temperature not specified in the publication | EDTA-degrading bacterium BNC1 | |
0.047 | - |
ethylenediaminetetraacetate | mutant R68A, presence of Mg2+, pH 7.6, temperature not specified in the publication | EDTA-degrading bacterium BNC1 | |
0.0492 | - |
ethylenediaminetetraacetate | wild-type, presence of Mg2+, pH 7.6, temperature not specified in the publication | EDTA-degrading bacterium BNC1 | |
0.0615 | - |
ethylenediaminetetraacetate | wild-type, presence of Mg2+ and flavin reductase from Escherichia coli, pH 7.6, temperature not specified in the publication | EDTA-degrading bacterium BNC1 | |
0.069 | - |
ethylenediaminetetraacetate | mutant Q294A, presence of Mg2+, pH 7.6, temperature not specified in the publication | EDTA-degrading bacterium BNC1 | |
0.198 | - |
ethylenediaminetetraacetate | mutant W11A, presence of Mg2+, pH 7.6, temperature not specified in the publication | EDTA-degrading bacterium BNC1 | |
0.49 | - |
ethylenediaminetetraacetate | mutant Q58A, presence of Mg2+, pH 7.6, temperature not specified in the publication | EDTA-degrading bacterium BNC1 | |
0.521 | - |
ethylenediaminetetraacetate | mutant P291A, presence of Mg2+, pH 7.6, temperature not specified in the publication | EDTA-degrading bacterium BNC1 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
EDTA-degrading bacterium BNC1 | Q9F9T3 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ethylenediaminetetraacetate + 2 FMNH2 + 2 O2 | - |
EDTA-degrading bacterium BNC1 | ethylenediamine-N,N'-diacetate + 2 glyoxylate + 2 FMN + 2 H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | light scattering experiments | EDTA-degrading bacterium BNC1 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.5 | - |
ethylenediaminetetraacetate | mutant W11A, presence of Mg2+, pH 7.6, temperature not specified in the publication | EDTA-degrading bacterium BNC1 | |
8.8 | - |
ethylenediaminetetraacetate | mutant Q58A, presence of Mg2+, pH 7.6, temperature not specified in the publication | EDTA-degrading bacterium BNC1 | |
12.8 | - |
ethylenediaminetetraacetate | mutant P291A, presence of Mg2+, pH 7.6, temperature not specified in the publication | EDTA-degrading bacterium BNC1 | |
13.2 | - |
ethylenediaminetetraacetate | wild-type, presence of Mg2+ and flavin reductase from Escherichia coli, pH 7.6, temperature not specified in the publication | EDTA-degrading bacterium BNC1 | |
14.7 | - |
ethylenediaminetetraacetate | mutant Q294A, presence of Mg2+, pH 7.6, temperature not specified in the publication | EDTA-degrading bacterium BNC1 | |
14.7 | - |
ethylenediaminetetraacetate | mutant R68A, presence of Mg2+, pH 7.6, temperature not specified in the publication | EDTA-degrading bacterium BNC1 | |
17.2 | - |
ethylenediaminetetraacetate | wild-type, presence of Mg2+, pH 7.6, temperature not specified in the publication | EDTA-degrading bacterium BNC1 | |
47.1 | - |
ethylenediaminetetraacetate | wild-type, presence of Mg2+ and oxidoreductase EmoB, pH 7.6, temperature not specified in the publication | EDTA-degrading bacterium BNC1 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
20 | - |
ethylenediaminetetraacetate | mutant P291A, presence of Mg2+, pH 7.6, temperature not specified in the publication | EDTA-degrading bacterium BNC1 | |
20 | - |
ethylenediaminetetraacetate | mutant Q58A, presence of Mg2+, pH 7.6, temperature not specified in the publication | EDTA-degrading bacterium BNC1 | |
20 | - |
ethylenediaminetetraacetate | mutant W11A, presence of Mg2+, pH 7.6, temperature not specified in the publication | EDTA-degrading bacterium BNC1 | |
31 | - |
ethylenediaminetetraacetate | mutant R68A, presence of Mg2+, pH 7.6, temperature not specified in the publication | EDTA-degrading bacterium BNC1 | |
210 | - |
ethylenediaminetetraacetate | mutant Q294A, presence of Mg2+, pH 7.6, temperature not specified in the publication | EDTA-degrading bacterium BNC1 | |
350 | - |
ethylenediaminetetraacetate | wild-type, presence of Mg2+, pH 7.6, temperature not specified in the publication | EDTA-degrading bacterium BNC1 |