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Literature summary for 1.14.14.34 extracted from

  • Kertesz, M.A.; Schmidt-Larbig, K.; Wueest, T.
    A novel reduced flavin mononucleotide-dependent methanesulfonate sulfonatase encoded by the sulfur-regulated msu operon of Pseudomonas aeruginosa (1999), J. Bacteriol., 181, 1464-1473.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41600
-
-
Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa Q9I1C2
-
-
Pseudomonas aeruginosa DSM 22644 Q9I1C2
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ethanesulfonate + FMNH2 + O2 27% of the activity with methanesulfonate Pseudomonas aeruginosa acetaldehyde + FMN + sulfite + H2O
-
?
ethanesulfonate + FMNH2 + O2 27% of the activity with methanesulfonate Pseudomonas aeruginosa DSM 22644 acetaldehyde + FMN + sulfite + H2O
-
?
isethionate + FMNH2 + O2 19% of the activity with methanesulfonate Pseudomonas aeruginosa hydroxyacetaldehyde + FMN + sulfite + H2O
-
?
isethionate + FMNH2 + O2 19% of the activity with methanesulfonate Pseudomonas aeruginosa DSM 22644 hydroxyacetaldehyde + FMN + sulfite + H2O
-
?
methanesulfonate + FMNH2 + O2
-
Pseudomonas aeruginosa formaldehyde + FMN + sulfite + H2O
-
?
methanesulfonate + FMNH2 + O2
-
Pseudomonas aeruginosa DSM 22644 formaldehyde + FMN + sulfite + H2O
-
?
morpholinepropanesulfonic acid + FMNH2 + O2 17% of the activity with methanesulfonate Pseudomonas aeruginosa 3-(morpholin-4-yl)propanal + FMN + sulfite + H2O
-
?
morpholinepropanesulfonic acid + FMNH2 + O2 17% of the activity with methanesulfonate Pseudomonas aeruginosa DSM 22644 3-(morpholin-4-yl)propanal + FMN + sulfite + H2O
-
?
pentanesulfonate + FMNH2 + O2 13% of the activity with methanesulfonate Pseudomonas aeruginosa pentanal + FMN + sulfite + H2O
-
?
pentanesulfonate + FMNH2 + O2 13% of the activity with methanesulfonate Pseudomonas aeruginosa DSM 22644 pentanal + FMN + sulfite + H2O
-
?
piperazine-N,N'-bis(2-ethanesulfonic acid) + 2 FMNH2 + 2 O2 31% of the activity with methanesulfonate Pseudomonas aeruginosa 2,2'-piperazine-1,4-diyldiacetaldehyde + 2 FMN + 2 sulfite + 2 H2O
-
?

Subunits

Subunits Comment Organism
? x * 41600, calculated Pseudomonas aeruginosa

Synonyms

Synonyms Comment Organism
msuD
-
Pseudomonas aeruginosa

pI Value

Organism Comment pI Value Maximum pI Value
Pseudomonas aeruginosa calculated
-
6.6

Expression

Organism Comment Expression
Pseudomonas aeruginosa expression of the msu operon is repressed in the presence of sulfate, sulfite, sulfide, or cysteine down
Pseudomonas aeruginosa growth with methanesulfonate requires an intact cysB gene, and the msu operon is part of the cys regulon additional information
Pseudomonas aeruginosa expression of the msu operon is derepressed during growth with methionine or alkanesulfonates up

General Information

General Information Comment Organism
physiological function the MsuD protein catalyzes the desulfonation of alkanesulfonates, requiring oxygen and FMNH2 for the reaction, and shows highest activity with methanesulfonate. NADH-dependent flavin mononucleotide reductase MsuE provides reduced FMN for the MsuD enzyme. Putative monooxygenase MsuC is involved but not essential in the desulfonation process Pseudomonas aeruginosa