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Literature summary for 1.14.14.36 extracted from

  • Kahn, R.A.; Fahrendorf, T.; Halkier, B.A.; Moller, B.L.
    Substrate specificity of the cytochrome P450 enzymes CYP79A1 and CYP71E1 involved in the biosynthesis of the cyanogenic glucoside dhurrin in Sorghum bicolor (L.) Moench (1999), Arch. Biochem. Biophys., 363, 9-18.
    View publication on PubMed
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General Stability

General Stability Organism
fairly stable Sorghum bicolor

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.013
-
 NADPH
-
 Sorghum bicolor
0.3
-
 NADH
-
 Sorghum bicolor

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Sorghum bicolor
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-tyrosine + O2 + [reduced NADPH-hemoprotein reductase] Sorghum bicolor enzyme in biosynthesis of cyanogenic glucosides N-hydroxy-L-tyrosine + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Sorghum bicolor
-
-
-
Sorghum bicolor Q43135
-
-

Source Tissue

Source Tissue Comment Organism Textmining
seedling
-
 Sorghum bicolor
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-tyrosine + 2 O2 + 2 [reduced NADPH-hemoprotein reductase]
-
 Sorghum bicolor (E)-[4-hydroxyphenylacetaldehyde oxime] + 2 [oxidized NADPH-hemoprotein reductase] + CO2 + 3 H2O overall reaction. NADPH is a much better cofactor for NADPH-hemoprotein reductase than NADH although NADH does support the entire catalytic cycle ?
L-tyrosine + 2 O2 + 2 [reduced NADPH-hemoprotein reductase] intermediate products: N-hydroxytyrosine, N,N-dihydroxytyrosine, (E)-p-hydroxyphenylacetaldoxime Sorghum bicolor (E)-[4-hydroxyphenylacetaldehyde oxime] + 2 [oxidized NADPH-hemoprotein reductase] + CO2 + 3 H2O
-
 ?
L-tyrosine + O2 + NADH + H+
-
 Sorghum bicolor N-hydroxy-L-tyrosine + NAD+ + H2O
-
 r
L-tyrosine + O2 + NADPH + H+
-
 Sorghum bicolor N-hydroxy-L-tyrosine + NADP+ + H2O
-
 r
L-tyrosine + O2 + [reduced NADPH-hemoprotein reductase] enzyme in biosynthesis of cyanogenic glucosides Sorghum bicolor N-hydroxy-L-tyrosine + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
additional information CYP79A1 has a very high substrate specificity, tyrosine being the only substrate found. Analogs 1-nitro-2-p-(hydroxyphenyl)ethane, N-hydroxytyrosine and phenylalanine are able to bind to the enzyme, without being metabolized Sorghum bicolor ?
-
 ?

Cofactor

Cofactor Comment Organism Structure
NADPH better cofactor than NADH Sorghum bicolor