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Literature summary for 1.14.14.39 extracted from

  • Andersen, M.D.; Busk, P.K.; Svendsen, I.; Moller, B.L.
    Cytochromes P-450 from cassava (Manihot esculenta Crantz) catalyzing the first steps in the biosynthesis of the cyanogenic glucosides linamarin and lotaustralin: Cloning, functional expression in Pichia pastoris, and substrate specificity of the isolated recombinant enzymes (2000), J. Biol. Chem., 275, 1966-1975.
    View publication on PubMed
Search for more literature for 1.14.14.39

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris Manihot esculenta

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3
-
 L-isoleucine pH 7.9, 30°C Manihot esculenta

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Manihot esculenta
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
61200
-
-
 Manihot esculenta
62000
-
-
 Manihot esculenta

Organism

Organism UniProt Comment Textmining
Manihot esculenta Q9M7B7 cf. EC 1.14.14.38
-
Manihot esculenta Q9M7B8 cf. EC 1.14.14.38
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein recombinant protein expressed in Pichia pastoris is glycosylated Manihot esculenta

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Manihot esculenta
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-isoleucine + 2 O2 + 2 [reduced NADPH-hemoprotein reductase]
-
 Manihot esculenta (1E,2S)-2-methylbutanal oxime + 2 [oxidized NADPH-hemoprotein reductase] + CO2 + 3 H2O overall reaction ?
additional information enzyme additionally acts on L-valine, reaction of EC 1.14.14.38. No substrates: D-valine, D-isoleucine, L-leucine, L-phenylalanine, or L-tyrosine Manihot esculenta ?
-
 ?
additional information enzyme additionally acts on L-valine, reaction of EC 1.14.14.38. The conversion rate of L-isoleucine is approximately 60% of that observed for L-valine. No substrates: D-valine, D-isoleucine, L-leucine, L-phenylalanine, or L-tyrosine Manihot esculenta ?
-
 ?

Subunits

Subunits Comment Organism
? x * 61200, calculated, x * 62000, SDS-PAGE Manihot esculenta

Synonyms

Synonyms Comment Organism
CYP79D1
-
 Manihot esculenta
CYP79D2
-
 Manihot esculenta
valine N-monooxygenase
-
 Manihot esculenta

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.1
-
 L-isoleucine pH 7.9, 30°C Manihot esculenta

General Information

General Information Comment Organism
physiological function bifunctional enzyme, metabolizes L-valine as well as L-isoleucine, i.e. activities of EC 1.14.14.38 and 1.14.14.39, consistent with the cooccurrence of linamarin and lotaustralin in cassava Manihot esculenta
physiological function bifunctional enzyme, metabolizes L-valine as well as L-isoleucine, i.e. activities of EC 1.14.14.38 and 1.14.14.39, consistent with the cooccurrence of linamarin and lotaustralin in cassava. CYP79D1 has a higher kcat value with L-valine as substrate than with L-isoleucine, which is consistent with linamarin being the major cyanogenic glucoside in cassava Manihot esculenta