Cloned (Comment) | Organism |
---|---|
expression in Saccharomyces cerevisiae | Lotus japonicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
L-isoleucine | 28°C, pH 7.5 | Lotus japonicus | |
0.7 | - |
L-isoleucine | pH 7.5, 28°C | Lotus japonicus | |
1.7 | - |
L-valine | 28°C, pH 7.5 | Lotus japonicus | |
1.8 | - |
L-isoleucine | 28°C, pH 7.5 | Lotus japonicus | |
1.8 | - |
L-isoleucine | pH 7.5, 28°C | Lotus japonicus | |
2.6 | - |
L-valine | 28°C, pH 7.5 | Lotus japonicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Lotus japonicus | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lotus japonicus | Q6J540 | - |
- |
Lotus japonicus | Q6J540 | isoform CYP79D4 | - |
Lotus japonicus | Q6J541 | - |
- |
Lotus japonicus | Q6J541 | isoform CYP79D3 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | apical leaf, highest enzymic activity among the tissues tested. Also present in the second leaf from top, no transcripts in older leaves | Lotus japonicus | - |
additional information | exclusively expressed in the aerial parts of the plant | Lotus japonicus | - |
additional information | exclusively expressed in aerial parts | Lotus japonicus | - |
additional information | exclusively expressed in root | Lotus japonicus | - |
root | - |
Lotus japonicus | - |
stem | - |
Lotus japonicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-isoleucine + 2 O2 + 2 [reduced NADPH-hemoprotein reductase] | - |
Lotus japonicus | (1E,2S)-2-methylbutanal oxime + 2 [oxidized NADPH-hemoprotein reductase] + CO2 + 3 H2O | overall reaction | ? | |
L-isoleucine + 2 [reduced NADPH-hemoprotein reductase] + 2 O2 | higher catalytic efficiency with L-Ile as substrate than with L-Val, in agreement with lotaustralin and rhodiocyanoside A and D being the major cyanogenic and nitrile glucosides in Lotus japonicus | Lotus japonicus | (1E,2S)-2-methylbutanal oxime + 2 [oxidized NADPH-hemoprotein reductase] + CO2 + 3 H2O | - |
? | |
L-valine + 2 [reduced NADPH-hemoprotein reductase] + 2 O2 | higher catalytic efficiency with L-Ile as substrate than with L-Val, in agreement with lotaustralin and rhodiocyanoside A and D being the major cyanogenic and nitrile glucosides in Lotus japonicus | Lotus japonicus | (E)-2-methylpropanal oxime + 2 [oxidized NADPH-hemoprotein reductase] + CO2 + 3 H2O | - |
? | |
additional information | metabolism of L-Leu, L-Phe, or L-Tyr to the corresponding oximes is not detectable in consistence with the absence of cyanogenic glucosides derived from these amino acids. No substrate: L-Trp, L-Met, L-Pro | Lotus japonicus | ? | - |
? | |
additional information | enzyme additionally acts on L-valine, reaction of EC 1.14.14.38, the catalytic efficiency (Kcat/Km) being 6fold higher with L-Ile than with L-Val as substrate. No substrates: L-Tyr, L-Phe, L-Leu, L-Trp, L-Met, and L-Pro | Lotus japonicus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CYP79D3 | - |
Lotus japonicus |
CYP79D4 | - |
Lotus japonicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.73 | - |
L-valine | 28°C, pH 7.5 | Lotus japonicus | |
0.83 | - |
L-valine | 28°C, pH 7.5 | Lotus japonicus | |
2 | - |
L-isoleucine | 28°C, pH 7.5 | Lotus japonicus | |
2 | - |
L-isoleucine | pH 7.5, 28°C | Lotus japonicus | |
3.67 | - |
L-isoleucine | 28°C, pH 7.5 | Lotus japonicus | |
3.67 | - |
L-isoleucine | pH 7.5, 28°C | Lotus japonicus |
General Information | Comment | Organism |
---|---|---|
physiological function | enzyme is part of the biosynthetic pathway leading to nitrile glucosides rhodiocyanoside A and rhodiocyanoside D as well as the cyanogenic glucosides linamarin and lotaustralin. Lotaustralin, rhodiocyanoside A, and rhodiocyanoside D are derived from the amino acid L-Ile, whereas linamarin is derived from L-Val | Lotus japonicus |
physiological function | enzyme catalyzes the conversion of Val and Ile to the corresponding aldoximes in biosynthesis of cyanogenic glucosides and nitrile glucosides in Lotus japonicus. Recombinantly expressed isoforms CYP79D3 and CYP79D4 in yeast cells show higher catalytic efficiency with L-Ile as substrate than with L-Val, in agreement with lotaustralin and rhodiocyanoside A and D being the major cyanogenic and nitrile glucosides in Lotus japonicus | Lotus japonicus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.32 | - |
L-valine | 28°C, pH 7.5 | Lotus japonicus | |
0.43 | - |
L-valine | 28°C, pH 7.5 | Lotus japonicus | |
2 | - |
L-isoleucine | pH 7.5, 28°C | Lotus japonicus | |
2.03 | - |
L-isoleucine | 28°C, pH 7.5 | Lotus japonicus | |
2.85 | - |
L-isoleucine | 28°C, pH 7.5 | Lotus japonicus | |
2.85 | - |
L-isoleucine | pH 7.5, 28°C | Lotus japonicus |