BRENDA - Enzyme Database show
show all sequences of 1.14.14.40

Cytochrome P450 CYP79A2 from Arabidopsis thaliana L. catalyzes the conversion of L-phenylalanine to phenylacetaldoxime in the biosynthesis of benzylglucosinolate

Wittstock, U.; Halkier, B.; J. Biol. Chem. 275, 14659-14666 (2000)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
glutathione
reduced glutathione, final concentration of 3 mM, slight stimulation
Arabidopsis thaliana
reduced glutathione
addition of reduced glutathione to a final concentration of 3 mM stimulates aldoxime production
Arabidopsis thaliana
Tween 80
addition of Tween 80 to a final concentration of 0.083% (v/v) results in a 1.5fold increase in aldoxime production; final concentration of 0.083% (v/v), 1.5fold increase in aldoxime production
Arabidopsis thaliana
Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli JM109 cells; expression in Escherichia coli
Arabidopsis thaliana
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0067
-
L-phenylalanine
pH 7.5, 26C
Arabidopsis thaliana
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
60000
-
x * 60000, SDS-PAGE
Arabidopsis thaliana
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arabidopsis thaliana
Q9FLC8
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-phenylalanine + 2 O2 + 2 [reduced NADPH-hemoprotein reductase]
-
715445
Arabidopsis thaliana
(E)-phenylacetaldoxime + 2 [oxidized NADPH-hemoprotein reductase] + CO2 + 3 H2O
overall reaction
-
-
?
L-phenylalanine + 2 [reduced NADPH-hemoprotein reductase] + 2 O2
-
715445
Arabidopsis thaliana
(E)-phenylacetaldoxime + 2 [oxidized NADPH-hemoprotein reductase] + CO2 + 3 H2O
-
-
-
?
additional information
neither L-tyrosine, L-tryptophan, L-methionine, nor DL-homophenylalanine are metabolized by CYP79A2
715445
Arabidopsis thaliana
?
-
-
-
-
additional information
no substrates: L-tyrosine, L-tryptophan, L-methionine, or DL-homophenylalanine
715445
Arabidopsis thaliana
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
?
x * 60000, SDS-PAGE
Arabidopsis thaliana
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Arabidopsis thaliana
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6
7.9
compared with the activity at pH 7.5, the activity of chimeric CYP79A2 is 25% at pH 6.0, 50% at pH 6.5, 80% at pH 7.0, and 70% at pH 7.9
Arabidopsis thaliana
6
-
25% of maximum activity
Arabidopsis thaliana
7.9
-
70% of maximum activity
Arabidopsis thaliana
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Arabidopsis thaliana
NADPH
-
Arabidopsis thaliana
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
glutathione
reduced glutathione, final concentration of 3 mM, slight stimulation
Arabidopsis thaliana
reduced glutathione
addition of reduced glutathione to a final concentration of 3 mM stimulates aldoxime production
Arabidopsis thaliana
Tween 80
addition of Tween 80 to a final concentration of 0.083% (v/v) results in a 1.5fold increase in aldoxime production; final concentration of 0.083% (v/v), 1.5fold increase in aldoxime production
Arabidopsis thaliana
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli JM109 cells; expression in Escherichia coli
Arabidopsis thaliana
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Arabidopsis thaliana
NADPH
-
Arabidopsis thaliana
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0067
-
L-phenylalanine
pH 7.5, 26C
Arabidopsis thaliana
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
60000
-
x * 60000, SDS-PAGE
Arabidopsis thaliana
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-phenylalanine + 2 O2 + 2 [reduced NADPH-hemoprotein reductase]
-
715445
Arabidopsis thaliana
(E)-phenylacetaldoxime + 2 [oxidized NADPH-hemoprotein reductase] + CO2 + 3 H2O
overall reaction
-
-
?
L-phenylalanine + 2 [reduced NADPH-hemoprotein reductase] + 2 O2
-
715445
Arabidopsis thaliana
(E)-phenylacetaldoxime + 2 [oxidized NADPH-hemoprotein reductase] + CO2 + 3 H2O
-
-
-
?
additional information
neither L-tyrosine, L-tryptophan, L-methionine, nor DL-homophenylalanine are metabolized by CYP79A2
715445
Arabidopsis thaliana
?
-
-
-
-
additional information
no substrates: L-tyrosine, L-tryptophan, L-methionine, or DL-homophenylalanine
715445
Arabidopsis thaliana
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 60000, SDS-PAGE
Arabidopsis thaliana
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Arabidopsis thaliana
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6
7.9
compared with the activity at pH 7.5, the activity of chimeric CYP79A2 is 25% at pH 6.0, 50% at pH 6.5, 80% at pH 7.0, and 70% at pH 7.9
Arabidopsis thaliana
6
-
25% of maximum activity
Arabidopsis thaliana
7.9
-
70% of maximum activity
Arabidopsis thaliana
General Information
General Information
Commentary
Organism
metabolism
CYP79A2 is involved in the biosynthesis of benzylglucosinolate
Arabidopsis thaliana
physiological function
enzyme is an N-hydroxylase converting L-phenylalanine into phenylacetaldoxime, the precursor of benzylglucosinolate. Transgenic Arabidopsis thaliana constitutively expressing CYP79A2 accumulate high levels of benzylglucosinolate
Arabidopsis thaliana
General Information (protein specific)
General Information
Commentary
Organism
metabolism
CYP79A2 is involved in the biosynthesis of benzylglucosinolate
Arabidopsis thaliana
physiological function
enzyme is an N-hydroxylase converting L-phenylalanine into phenylacetaldoxime, the precursor of benzylglucosinolate. Transgenic Arabidopsis thaliana constitutively expressing CYP79A2 accumulate high levels of benzylglucosinolate
Arabidopsis thaliana
Other publictions for EC 1.14.14.40
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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715445
Wittstock
Cytochrome P450 CYP79A2 from A ...
Arabidopsis thaliana
J. Biol. Chem.
275
14659-14666
2000
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