BRENDA - Enzyme Database show
show all sequences of 1.14.14.40

Identification and characterization of CYP79D16 and CYP71AN24 catalyzing the first and second steps in L-phenylalanine-derived cyanogenic glycoside biosynthesis in the Japanese apricot, Prunus mume Sieb. et Zucc

Yamaguchi, T.; Yamamoto, K.; Asano, Y.; Plant Mol. Biol. 86, 215-223 (2014)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Saccharomyces cerevisiae
Prunus mume
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Prunus mume
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Prunus mume
A0A068Q7V0
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
seedling
expressed specifically in the seedling, coexpression with CYP71AN24 which catalyzes the conversion of phenylacetaldoxime into mandelonitrile with broad substrate specificity
Prunus mume
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-phenylalanine + 2 O2 + 2 [reduced NADPH-hemoprotein reductase]
-
741183
Prunus mume
(E)-phenylacetaldoxime + 2 [oxidized NADPH-hemoprotein reductase] + CO2 + 3 H2O
overall reaction
-
-
?
additional information
the enzyme is specific for L-phenylalanine. No substrates: L-Tyr, L-Trp, and L-Val
741183
Prunus mume
?
-
-
-
-
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Prunus mume
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Saccharomyces cerevisiae
Prunus mume
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Prunus mume
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Prunus mume
-
-
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
seedling
expressed specifically in the seedling, coexpression with CYP71AN24 which catalyzes the conversion of phenylacetaldoxime into mandelonitrile with broad substrate specificity
Prunus mume
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-phenylalanine + 2 O2 + 2 [reduced NADPH-hemoprotein reductase]
-
741183
Prunus mume
(E)-phenylacetaldoxime + 2 [oxidized NADPH-hemoprotein reductase] + CO2 + 3 H2O
overall reaction
-
-
?
additional information
the enzyme is specific for L-phenylalanine. No substrates: L-Tyr, L-Trp, and L-Val
741183
Prunus mume
?
-
-
-
-
Other publictions for EC 1.14.14.40
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739318
Clausen
The bifurcation of the cyanoge ...
Sorghum bicolor
Plant J.
84
558-573
2015
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-
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4
-
1
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5
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4
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5
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729046
Miki
Construction of the biosynthet ...
Arabidopsis thaliana
Appl. Environ. Microbiol.
80
6828-6836
2014
-
1
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5
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1
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741183
Yamaguchi
Identification and characteriz ...
Prunus mume
Plant Mol. Biol.
86
215-223
2014
-
-
1
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1
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4
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1
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1
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1
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2
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715445
Wittstock
Cytochrome P450 CYP79A2 from A ...
Arabidopsis thaliana
J. Biol. Chem.
275
14659-14666
2000
3
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1
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1
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1
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4
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4
1
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1
3
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2
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3
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1
2
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1
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1
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4
1
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1
3
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2
2
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