BRENDA - Enzyme Database show
show all sequences of 1.14.14.42

Cytochrome p450 CYP79F1 from Arabidopsis catalyzes the conversion of dihomomethionine and trihomomethionine to the corresponding aldoximes in the biosynthesis of aliphatic glucosinolates

Hansen, C.H.; Wittstock, U.; Olsen, C.E.; Hick, A.J.; Pickett, J.A.; Halkier, B.A.; J. Biol. Chem. 276, 11078-11085 (2001)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Arabidopsis thaliana
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-dihomomethionine + 2 O2 + 2 [reduced NADPH-hemoprotein reductase]
Arabidopsis thaliana
-
omega-(methylthio)-(E)-pentanaldehyde oxime + CO2 + 3 H2O + 2 [oxidized NADPH-hemoprotein reductase]
overall reaction, product is the precursor of the major glucosinolates in leaves of Arabidopsis thaliana
-
?
L-trihomomethionine + 2 O2 + 2 [reduced NADPH-hemoprotein reductase]
Arabidopsis thaliana
-
omega-(methylthio)-(E)-hexanaldehyde oxime + CO2 + 3 H2O + 2 [oxidized NADPH-hemoprotein reductase]
overall reaction
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arabidopsis thaliana
Q949U1
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-dihomomethionine + 2 O2 + 2 [reduced NADPH-hemoprotein reductase]
-
740667
Arabidopsis thaliana
omega-(methylthio)-(E)-pentanaldehyde oxime + CO2 + 3 H2O + 2 [oxidized NADPH-hemoprotein reductase]
overall reaction, product is the precursor of the major glucosinolates in leaves of Arabidopsis thaliana
-
-
?
L-trihomomethionine + 2 O2 + 2 [reduced NADPH-hemoprotein reductase]
-
740667
Arabidopsis thaliana
omega-(methylthio)-(E)-hexanaldehyde oxime + CO2 + 3 H2O + 2 [oxidized NADPH-hemoprotein reductase]
overall reaction
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Arabidopsis thaliana
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Arabidopsis thaliana
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Arabidopsis thaliana
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-dihomomethionine + 2 O2 + 2 [reduced NADPH-hemoprotein reductase]
Arabidopsis thaliana
-
omega-(methylthio)-(E)-pentanaldehyde oxime + CO2 + 3 H2O + 2 [oxidized NADPH-hemoprotein reductase]
overall reaction, product is the precursor of the major glucosinolates in leaves of Arabidopsis thaliana
-
?
L-trihomomethionine + 2 O2 + 2 [reduced NADPH-hemoprotein reductase]
Arabidopsis thaliana
-
omega-(methylthio)-(E)-hexanaldehyde oxime + CO2 + 3 H2O + 2 [oxidized NADPH-hemoprotein reductase]
overall reaction
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-dihomomethionine + 2 O2 + 2 [reduced NADPH-hemoprotein reductase]
-
740667
Arabidopsis thaliana
omega-(methylthio)-(E)-pentanaldehyde oxime + CO2 + 3 H2O + 2 [oxidized NADPH-hemoprotein reductase]
overall reaction, product is the precursor of the major glucosinolates in leaves of Arabidopsis thaliana
-
-
?
L-trihomomethionine + 2 O2 + 2 [reduced NADPH-hemoprotein reductase]
-
740667
Arabidopsis thaliana
omega-(methylthio)-(E)-hexanaldehyde oxime + CO2 + 3 H2O + 2 [oxidized NADPH-hemoprotein reductase]
overall reaction
-
-
?
General Information
General Information
Commentary
Organism
physiological function
transgenic Arabidopsis thaliana with cosuppression of CYP79F1 have a reduced content of aliphatic glucosinolates and a highly increased level of dihomomethionine and trihomomethionine. The transgenic plants have a morphological phenotype showing loss of apical dominance and formation of multiple axillary shoots
Arabidopsis thaliana
General Information (protein specific)
General Information
Commentary
Organism
physiological function
transgenic Arabidopsis thaliana with cosuppression of CYP79F1 have a reduced content of aliphatic glucosinolates and a highly increased level of dihomomethionine and trihomomethionine. The transgenic plants have a morphological phenotype showing loss of apical dominance and formation of multiple axillary shoots
Arabidopsis thaliana
Other publictions for EC 1.14.14.42
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741031
Yin
Molecular cloning, expression ...
Brassica oleracea var. alboglabra
Molecules
20
20254-20267
2015
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1
1
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1
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4
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6
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1
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6
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1
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1
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741170
Chen
CYP79F1 and CYP79F2 have disti ...
Arabidopsis thaliana
Plant J.
33
923-937
2003
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1
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7
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1
10
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7
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6
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10
1
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1
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2
1
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7
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1
10
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6
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10
1
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-
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1
2
-
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-
740667
Hansen
Cytochrome p450 CYP79F1 from A ...
Arabidopsis thaliana
J. Biol. Chem.
276
11078-11085
2001
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1
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2
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1
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1
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2
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2
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1
1
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