BRENDA - Enzyme Database show
show all sequences of 1.14.14.42

CYP79F1 and CYP79F2 have distinct functions in the biosynthesis of aliphatic glucosinolates in Arabidopsis

Chen, S.; Glawischnig, E.; Jörgensen, K.; Naur, P.; Jörgensen, B.; Olsen, C.E.; Hansen, C.H.; Rasmussen, H.; Pickett, J.A.; Halkier, B.A.; Plant J. 33, 923-937 (2003)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli; expression in Saccharomyces cerevisiae
Arabidopsis thaliana
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.026
-
L-hexahomomethionine
pH 7.5, 25°C
Arabidopsis thaliana
0.034
-
L-dihomomethionine
pH 7.5, 25°C
Arabidopsis thaliana
0.037
-
L-trihomomethionine
pH 7.5, 25°C
Arabidopsis thaliana
0.074
-
L-hexahomomethionine
pH 7.5, 25°C
Arabidopsis thaliana
0.194
-
L-tetrahomomethionine
pH 7.5, 25°C
Arabidopsis thaliana
0.216
-
L-pentahomomethionine
pH 7.5, 25°C
Arabidopsis thaliana
0.374
-
L-pentahomomethionine
pH 7.5, 25°C
Arabidopsis thaliana
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
62000
-
-
Arabidopsis thaliana
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-dihomomethionine + 2 O2 + 2 [reduced NADPH-hemoprotein reductase]
Arabidopsis thaliana
-
4-methylthiopentanaldoxime + CO2 + 3 H2O + 2 [oxidized NADPH-hemoprotein reductase]
-
-
?
L-hexahomomethionine + 2 O2 + 2 [reduced NADPH-hemoprotein reductase]
Arabidopsis thaliana
-
4-methylthiononanaldoxime + CO2 + 3 H2O + 2 [oxidized NADPH-hemoprotein reductase]
overall reaction
-
?
L-homomethionine + 2 O2 + 2 [reduced NADPH-hemoprotein reductase]
Arabidopsis thaliana
-
4-methylthiobutanaldoxime + CO2 + 3 H2O + 2 [oxidized NADPH-hemoprotein reductase]
-
-
?
L-pentahomomethionine + 2 O2 + 2 [reduced NADPH-hemoprotein reductase]
Arabidopsis thaliana
-
4-methylthiooctanaldoxime + CO2 + 3 H2O + 2 [oxidized NADPH-hemoprotein reductase]
overall reaction
-
?
L-tetrahomomethionine + 2 O2 + 2 [reduced NADPH-hemoprotein reductase]
Arabidopsis thaliana
-
4-methylthioheptanaldoxime + CO2 + 3 H2O + 2 [oxidized NADPH-hemoprotein reductase]
overall reaction
-
?
L-trihomomethionine + 2 O2 + 2 [reduced NADPH-hemoprotein reductase]
Arabidopsis thaliana
-
4-methylthiohexanaldoxime + CO2 + 3 H2O + 2 [oxidized NADPH-hemoprotein reductase]
overall reaction
-
?
additional information
Arabidopsis thaliana
isoform CYP79F1 metabolizes mono- to hexahomomethionine, resulting in both short- and long-chain aliphatic glucosinolates. No substrates: phenylalanine,homophenylalanine, tyrosine, tryptophan, and methionine
?
-
-
-
additional information
Arabidopsis thaliana
isoform CYP79F2 exclusively metabolizes long-chain elongated penta- and hexahomomethionines. No substrates: phenylalanine, homophenylalanine, tyrosine, tryptophan, and methionine
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arabidopsis thaliana
Q949U1
-
-
Arabidopsis thaliana
Q9FUY7
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cotyledon
strongly expressed in cotyledons, rosette leaves, stems, and siliques
Arabidopsis thaliana
-
hypocotyl
strongly expressed in hypocotyl and root
Arabidopsis thaliana
-
leaf
strongly expressed in cotyledons, rosette leaves, stems, and siliques
Arabidopsis thaliana
-
root
strongly expressed in hypocotyl and root
Arabidopsis thaliana
-
silique
strongly expressed in cotyledons, rosette leaves, stems, and siliques
Arabidopsis thaliana
-
stem
strongly expressed in cotyledons, rosette leaves, stems, and siliques
Arabidopsis thaliana
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-dihomomethionine + 2 O2 + 2 [reduced NADPH-hemoprotein reductase]
-
741170
Arabidopsis thaliana
4-methylthiopentanaldoxime + CO2 + 3 H2O + 2 [oxidized NADPH-hemoprotein reductase]
-
-
-
?
L-hexahomomethionine + 2 O2 + 2 [reduced NADPH-hemoprotein reductase]
-
741170
Arabidopsis thaliana
4-methylthiononanaldoxime + CO2 + 3 H2O + 2 [oxidized NADPH-hemoprotein reductase]
overall reaction
-
-
?
L-homomethionine + 2 O2 + 2 [reduced NADPH-hemoprotein reductase]
-
741170
Arabidopsis thaliana
4-methylthiobutanaldoxime + CO2 + 3 H2O + 2 [oxidized NADPH-hemoprotein reductase]
-
-
-
?
L-pentahomomethionine + 2 O2 + 2 [reduced NADPH-hemoprotein reductase]
-
741170
Arabidopsis thaliana
4-methylthiooctanaldoxime + CO2 + 3 H2O + 2 [oxidized NADPH-hemoprotein reductase]
overall reaction
-
-
?
L-tetrahomomethionine + 2 O2 + 2 [reduced NADPH-hemoprotein reductase]
-
741170
Arabidopsis thaliana
4-methylthioheptanaldoxime + CO2 + 3 H2O + 2 [oxidized NADPH-hemoprotein reductase]
overall reaction
-
-
?
L-trihomomethionine + 2 O2 + 2 [reduced NADPH-hemoprotein reductase]
-
741170
Arabidopsis thaliana
4-methylthiohexanaldoxime + CO2 + 3 H2O + 2 [oxidized NADPH-hemoprotein reductase]
overall reaction
-
-
?
additional information
isoform CYP79F1 metabolizes mono- to hexahomomethionine, resulting in both short- and long-chain aliphatic glucosinolates. No substrates: phenylalanine,homophenylalanine, tyrosine, tryptophan, and methionine
741170
Arabidopsis thaliana
?
-
-
-
-
additional information
isoform CYP79F2 exclusively metabolizes long-chain elongated penta- and hexahomomethionines. No substrates: phenylalanine, homophenylalanine, tyrosine, tryptophan, and methionine
741170
Arabidopsis thaliana
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
?
x * 62000, calculated and SDS-PAGE
Arabidopsis thaliana
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Arabidopsis thaliana
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Arabidopsis thaliana
expression in Saccharomyces cerevisiae
Arabidopsis thaliana
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Arabidopsis thaliana
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.026
-
L-hexahomomethionine
pH 7.5, 25°C
Arabidopsis thaliana
0.034
-
L-dihomomethionine
pH 7.5, 25°C
Arabidopsis thaliana
0.037
-
L-trihomomethionine
pH 7.5, 25°C
Arabidopsis thaliana
0.074
-
L-hexahomomethionine
pH 7.5, 25°C
Arabidopsis thaliana
0.194
-
L-tetrahomomethionine
pH 7.5, 25°C
Arabidopsis thaliana
0.216
-
L-pentahomomethionine
pH 7.5, 25°C
Arabidopsis thaliana
0.374
-
L-pentahomomethionine
pH 7.5, 25°C
Arabidopsis thaliana
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
62000
-
-
Arabidopsis thaliana
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-dihomomethionine + 2 O2 + 2 [reduced NADPH-hemoprotein reductase]
Arabidopsis thaliana
-
4-methylthiopentanaldoxime + CO2 + 3 H2O + 2 [oxidized NADPH-hemoprotein reductase]
-
-
?
L-hexahomomethionine + 2 O2 + 2 [reduced NADPH-hemoprotein reductase]
Arabidopsis thaliana
-
4-methylthiononanaldoxime + CO2 + 3 H2O + 2 [oxidized NADPH-hemoprotein reductase]
overall reaction
-
?
L-homomethionine + 2 O2 + 2 [reduced NADPH-hemoprotein reductase]
Arabidopsis thaliana
-
4-methylthiobutanaldoxime + CO2 + 3 H2O + 2 [oxidized NADPH-hemoprotein reductase]
-
-
?
L-pentahomomethionine + 2 O2 + 2 [reduced NADPH-hemoprotein reductase]
Arabidopsis thaliana
-
4-methylthiooctanaldoxime + CO2 + 3 H2O + 2 [oxidized NADPH-hemoprotein reductase]
overall reaction
-
?
L-tetrahomomethionine + 2 O2 + 2 [reduced NADPH-hemoprotein reductase]
Arabidopsis thaliana
-
4-methylthioheptanaldoxime + CO2 + 3 H2O + 2 [oxidized NADPH-hemoprotein reductase]
overall reaction
-
?
L-trihomomethionine + 2 O2 + 2 [reduced NADPH-hemoprotein reductase]
Arabidopsis thaliana
-
4-methylthiohexanaldoxime + CO2 + 3 H2O + 2 [oxidized NADPH-hemoprotein reductase]
overall reaction
-
?
additional information
Arabidopsis thaliana
isoform CYP79F1 metabolizes mono- to hexahomomethionine, resulting in both short- and long-chain aliphatic glucosinolates. No substrates: phenylalanine,homophenylalanine, tyrosine, tryptophan, and methionine
?
-
-
-
additional information
Arabidopsis thaliana
isoform CYP79F2 exclusively metabolizes long-chain elongated penta- and hexahomomethionines. No substrates: phenylalanine, homophenylalanine, tyrosine, tryptophan, and methionine
?
-
-
-
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cotyledon
strongly expressed in cotyledons, rosette leaves, stems, and siliques
Arabidopsis thaliana
-
hypocotyl
strongly expressed in hypocotyl and root
Arabidopsis thaliana
-
leaf
strongly expressed in cotyledons, rosette leaves, stems, and siliques
Arabidopsis thaliana
-
root
strongly expressed in hypocotyl and root
Arabidopsis thaliana
-
silique
strongly expressed in cotyledons, rosette leaves, stems, and siliques
Arabidopsis thaliana
-
stem
strongly expressed in cotyledons, rosette leaves, stems, and siliques
Arabidopsis thaliana
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-dihomomethionine + 2 O2 + 2 [reduced NADPH-hemoprotein reductase]
-
741170
Arabidopsis thaliana
4-methylthiopentanaldoxime + CO2 + 3 H2O + 2 [oxidized NADPH-hemoprotein reductase]
-
-
-
?
L-hexahomomethionine + 2 O2 + 2 [reduced NADPH-hemoprotein reductase]
-
741170
Arabidopsis thaliana
4-methylthiononanaldoxime + CO2 + 3 H2O + 2 [oxidized NADPH-hemoprotein reductase]
overall reaction
-
-
?
L-homomethionine + 2 O2 + 2 [reduced NADPH-hemoprotein reductase]
-
741170
Arabidopsis thaliana
4-methylthiobutanaldoxime + CO2 + 3 H2O + 2 [oxidized NADPH-hemoprotein reductase]
-
-
-
?
L-pentahomomethionine + 2 O2 + 2 [reduced NADPH-hemoprotein reductase]
-
741170
Arabidopsis thaliana
4-methylthiooctanaldoxime + CO2 + 3 H2O + 2 [oxidized NADPH-hemoprotein reductase]
overall reaction
-
-
?
L-tetrahomomethionine + 2 O2 + 2 [reduced NADPH-hemoprotein reductase]
-
741170
Arabidopsis thaliana
4-methylthioheptanaldoxime + CO2 + 3 H2O + 2 [oxidized NADPH-hemoprotein reductase]
overall reaction
-
-
?
L-trihomomethionine + 2 O2 + 2 [reduced NADPH-hemoprotein reductase]
-
741170
Arabidopsis thaliana
4-methylthiohexanaldoxime + CO2 + 3 H2O + 2 [oxidized NADPH-hemoprotein reductase]
overall reaction
-
-
?
additional information
isoform CYP79F1 metabolizes mono- to hexahomomethionine, resulting in both short- and long-chain aliphatic glucosinolates. No substrates: phenylalanine,homophenylalanine, tyrosine, tryptophan, and methionine
741170
Arabidopsis thaliana
?
-
-
-
-
additional information
isoform CYP79F2 exclusively metabolizes long-chain elongated penta- and hexahomomethionines. No substrates: phenylalanine, homophenylalanine, tyrosine, tryptophan, and methionine
741170
Arabidopsis thaliana
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 62000, calculated and SDS-PAGE
Arabidopsis thaliana
General Information
General Information
Commentary
Organism
physiological function
a transposon-tagged CYP79F1 knockout mutant completely lacks short-chain aliphatic glucosinolates, but has an increased level of long-chain aliphatic glucosinolates, especially in leaves and seeds; the level of long-chain aliphatic glucosinolates in a transposon-tagged CYP79F2 knockout mutant is substantially reduced, whereas the level of short-chain aliphatic glucosinolates is not affected
Arabidopsis thaliana
General Information (protein specific)
General Information
Commentary
Organism
physiological function
a transposon-tagged CYP79F1 knockout mutant completely lacks short-chain aliphatic glucosinolates, but has an increased level of long-chain aliphatic glucosinolates, especially in leaves and seeds
Arabidopsis thaliana
physiological function
the level of long-chain aliphatic glucosinolates in a transposon-tagged CYP79F2 knockout mutant is substantially reduced, whereas the level of short-chain aliphatic glucosinolates is not affected
Arabidopsis thaliana
Other publictions for EC 1.14.14.42
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741031
Yin
Molecular cloning, expression ...
Brassica oleracea var. alboglabra
Molecules
20
20254-20267
2015
-
-
1
1
-
-
-
-
-
-
1
-
-
4
-
-
-
-
-
6
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
6
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
741170
Chen
CYP79F1 and CYP79F2 have disti ...
Arabidopsis thaliana
Plant J.
33
923-937
2003
-
-
1
-
-
-
-
7
-
-
1
10
-
7
-
-
-
-
-
6
-
-
10
1
-
-
-
-
-
-
-
1
-
-
-
-
-
2
1
-
-
-
-
-
-
7
-
-
1
10
-
-
-
-
-
6
-
-
10
1
-
-
-
-
-
-
-
-
-
1
2
-
-
-
740667
Hansen
Cytochrome p450 CYP79F1 from A ...
Arabidopsis thaliana
J. Biol. Chem.
276
11078-11085
2001
-
-
1
-
-
-
-
-
-
-
-
2
-
6
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-